The research advancement of fibroblast on diabetic non-healing skin wound
1
Citation
3
Reference
10
Related Paper
Citation Trend
Abstract:
The delayed wound healing especially non-healing skin wound is one of the problem in clinical practice and hot research in basic medicine.The common therapies' effects were not confirm.The induced Pluripotent Stem Cell (iPSC) technology is one of de novo approaches in regeneration medicine these years.The original iPSC was reprogrammed from rat tail fibroblast.So the concept of direct cellular reprogramming was reported versus the iPSC-based reprogramming.Thus we review the fibroblast potential "stem" characteristics and its promotion to the wound healing.Keywords:
Reprogramming
Regenerative Medicine
Induced pluripotent stem cells (iPSCs) were reprogrammed from somatic cells using specific transcription factors. Bypassing the ethical issue caused by embryonic stem cells (ESCs), iPSCs can be successfully induced from a variety of cells, which makes iPSCs a powerful research tool for developmental biology. iPSCs have also become indispensable to the research of life science due to their broad potential applications. However, it's a big challenge to obtain iPSCs with high quality and genetic stability. Here, we review the research progress of increasing the reprogramming mechanism and genetic stability of iPSCs in order to provide references of reprogramming efficiency of iPSCs, reducing the cost, and addressing key points of iPSCs quality control, further promoting clinical application of the iPSCs.
Reprogramming
Regenerative Medicine
Cite
Citations (1)
Reprogramming of induced pluripotent stem cells(iPSCs) is an important breakthrough in the field of regenerative medicine recently.It is achieved through transduction of exogenous transcription factors,into somatic cells,reprogramming the somatic cells to embryonic stem cells-like cells with potencies of unlimited proliferation and pluripotent differentiation.IPSCs derived from somatic cells of patients represent a powerful tool for biomedical research and may provide a source for replacement therapies.Here the research progress of iPSCs reprogramming and its medical prospect were emphasized.
Reprogramming
Regenerative Medicine
Induced stem cells
Cite
Citations (0)
Reprogramming
Ectopic expression
Cite
Citations (14)
Induced pluripotent stem cells (iPSCs) technology is a method for generating pluripotent stem cells in vitro from fully differentiated cells such as fibroblast cells. The potential applications of iPSC technology in cell therapy and disease modeling could influence current medical practices. Despite current advances in iPSC technology, many patient-derived reprogrammed cells are not suitable for clinical trial because most protocols rely on virus-based techniques, which pose the risk of integration of the viral genome into the chromosomes. Therefore, non-viral methods such as mRNA and protein-based reprogramming are promising alternatives when generating clinically safe iPSCs. In a previous study, we generated human iPSCs using cell extracts with cell penetration peptide (CPP) for the delivery of reprogramming proteins [Kim et al. Cell Stem Cells, 2009]. In here, we show that the expression of reprogramming factors in mammalian cells and subsequent purification of these factors by FLAG-Tag could reprogram fibroblasts into iPSCs.
Reprogramming
Cite
Citations (6)
Current advances in cellular reprogramming technology has demonstrated that the identity of a cell can be converted by the use of master transcription factors to reprogram the transcriptome.Notably, this allows us to convert somatic cells into induced pluripotent stem cells (iPSCs), providing a feasible method to generate patient-specific pluripotent stem cells.This technology was firstly discovered by Shinya Yamanaka's group in 2006.The initial iPSCs were formed by the induction of dedifferentiation in mouse fibroblasts using transcription factors: Oct4, Sox2, Klf4 and c-Myc.This approach has tremendous medical potentials to revolutionize the way we study and develop treatment for ocular diseases.Here we reviewed the potential of using patient-specific iPSCs for 3D disease modeling and various types of retinal disease modeling, cell replacement therapy and clinical trials, high-throughput screening test and drug toxicity testing.We also discussed the recent development of direct reprogramming and the future direction for utilising iPSCs and cellular reprogramming technology for eye research.
Key words:
Induced pluripotent stem cells; Cellular reprogramming; Retina; Disease modeling; Cell therapy; Drug screening
Reprogramming
KLF4
Cite
Citations (0)
Reprogramming
Cite
Citations (128)
Reprogramming
Ectopic expression
Cite
Citations (1)
Application of Patient-Specific Induced Pluripotent Stem Cells Produced by Somatic Cells Reprogramming for Treatment of Neurodegenerative Diseases
Reprogramming
Cite
Citations (3)
Induced pluripotent stem cells can differentiate into a variety of cell types, which promote the development of human disease model, drug toxicity screening and sources of autologous cells.However, there have been many problems in the induced pluripotent stem cells reprogramming, such as safety and low efficiency.Small molecules are considered as a promising method to improve the reprogramming processes of induced pluripotent stem cell, and more and more small molecules have been identified to maintain stem cell self-renewal, providing a new approach to produce the desired reprogramming cells.
Key words:
Induced pluripotent stem cell; Small molecule; Reprogramming
Reprogramming
Induced stem cells
Cite
Citations (0)