Paralytic shellfish poisoning (PSP) toxins could be neuromuscular paralysis agents, which could blocke the voltage-gated sodium channels, and then could cause holdback in nervous system transmission. Some bivalves could be tolerant of PSP toxins. The resistance mechanisms might be related to metabolism, biotransformation and the mutation of voltage-gated sodium channels, etc., in which the mutation of sodium channels might be the most important molecular mechanism of the resistance mechanism of bivalves.
Through the experiment,the organic silico is used to improve the performence of polymer cement mortar.At the same time,the influence of the organic silicon on the polymer cement mortar compressiveness,consistency and water absorption properties is studied.The experimental results indicate that the organic silicon influences the polymer cement mortar compressiveness,water absorption obviously.
Objective To investigate the clinical characteristics of children with traumatic spinal cord injury (SCI) admitted to a research rehabilitation center between 2011 and 2020, with a view to generate crucial data for understanding and prevention of pediatric traumatic SCI.
Though microalgae have been considered the potential resource for lipid production, native strains are unable to meet the industrial demand. Here, we aim to uncover the complex molecular relationship between algal growth and lipid accumulation. Transcriptomic analysis revealed the crucial role of plastidial fatty acid and triacylglycerol (TAG) biosynthetic machinery in lipid overproduction. The expression of key fatty acid biosynthetic genes such as acetyl-CoA carboxylase (ACCase), malonyl CoA‐acyl carrier protein transacylase (MCAT), 3-ketoacyl synthase (KAS), 3-ketoacyl-ACP reductase (KAR) increased during day 10-13 of cultivation, particularly plastidial TAG biosynthetic genes substantially increased. However, expression of genes involved in ER TAG biosynthesis increased only in the stationary phase, which implied the potential of plastidial TAG biosynthesis. This report provides a novel insight into the growth-phase dependent lipogenic orchestration, and also uncovers the signature genes and plastidial TAG biosynthesis that might be extrapolated for improving lipogenic traits of algae.
Due to great temperature susceptivity, aging and inferior fatigue resistance, the serviceability of asphalt materials has been limited in same degree. The modification becomes an effective way to improve the performance of asphalt material. The modification applying nanoclay can not only improve the properties of bitumen but also lower costs to a great extent, so it becomes the research focus recently. It is well known that fatigue is considered to be one of the most significant distresses in any flexible pavement which is subjected to repeated traffic loading or stress. So it is very significant to pavement designer to investigate the fatigue characteristics of asphalt binder. The present research has chosen organic nano-montmorillonite (OMMT) to prepare the nanoclay modified bitumen using high speed shearer in laboratory. The X-Ray Diffraction (XRD) test was used to analyse the dispersion structure in the modified bitumen. And the fatigue test was carried out to evaluate the fatigue resistance property of the modified bitumen by Dynamic Shear Rheometer (DSR). The stress loading mode was applied to simulate the actual traffic loading. Finally, the fatigue equations were educed to fit the experiment results. The research results show that the modified bitumen containing nanoclay suggests excellent fatigue resistance.
Objective: To confirm whetherBungarus multicinctus crude venom induces the apoptosis of K562 tumor cells and to find out the components inducing apoptosis of K562 cells from the crude venom. Methods: the crude venom separated and purified by cation exchange chromatography, and the effect of venoms on K562 was studied by MTT method and flow cytometry. Results: The crude venom began to kill K562 cells at than 8×l03ng/ml (the survival rate was 82.5%) concentration and the effect was more significant in 24 h when administrating 8×l05ng/ml (the survival rate was 29.4%) crude venom. Apoptotic bodies were observed in the K562 tumor cells by fluorescent microscopy after administration of 5 µg/ml cycloheximide (CHX) or the peak VI solution at about 8×105 ng/ml. The same results were detected by the flow cytometry. A sub-Gi peak appeared after administration of CHX or the sixth peak solution. Conclusion: The authors found that the venom can kill K562 tumor cells in time- and dose-dependent manner. However, the killing effect of the venom is not apoptosis. What's more, the peak VI solution, a component of the crude venom can induce the apoptosis of K562 tumor cells.
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