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Kazuma Takase

University of Tokyo

26

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A new beneficial mutation in pseudomonas sp. 61-3 polyhydroxyalkanoate (PHA) synthase for enhanced cellular content of 3-hydroxybutyrate-based PHA explored using its enzyme homolog as a mutation template.

2010 Bioscience, Biotechnology, and Biochemistry
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Production of short-chain-length/medium-chain-length polyhydroxyalkanoate (PHA) copolymer in the plastid of Arabidopsis thaliana using an engineered 3-ketoacyl-acyl carrier protein synthase III

2009 Biomacromolecules
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In vivo and in vitro characterization of Ser477X mutations in polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. 61-3 : Effects of beneficial mutations on enzymatic activity, substrate specificity, and molecular weight of PHA

2006 Biomacromolecules
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Synthesis of Short-chain-length/Medium-chain-length Polyhydroxyalkanoate (PHA) Copolymers in Peroxisome of the Transgenic Arabidopsis Thaliana Harboring the PHA Synthase Gene from Pseudomonas sp. 61-3

2006 Journal of Polymers and The Environment
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Deletion analysis of the subunit genes of V-type Na+-ATPase from Enterococcus hirae.

2006 Journal of Biochemistry
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Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109

2005 Applied and Environmental Microbiology
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Synergistic Effects of Glu130Asp Substitution in the Type II Polyhydroxyalkanoate (PHA) Synthase: Enhancement of PHA Production and Alteration of Polymer Molecular Weight

2005 Biomacromolecules
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An extra large insertion in the polyhydroxyalkanoate synthase from Delftia acidovorans DS-17: its deletion effects and relation to cellular proteolysis

2004 Fems Microbiology Letters
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2B11-5 Composition control of PHA copolymer by molecular evolutionary engineering of Pseudomonas sp. 61-3 PHA synthase

2004
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Alteration of substrate chain-length specificity of type II synthase for polyhydroxyalkanoate biosynthesis by in vitro evolution: in vivo and in vitro enzyme assays.

2004 Biomacromolecules
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Citations (100)