Topographic and chemical patterning of cell-surface interfaces to influence cellular functions
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Рассмотрены масочные и безмасочные методы функционализации для формирования рисунков на поверхностях различных материалов.The mask and mask-free methods of functionalization for the surface patterning of different materials are considered.
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This chapter contains sections titled: Introduction Overview: Background and History Physiology of Information Processing within Neuronal Networks Topographical Patterning Chemical Patterning Methods Topographical Patterning Photolithographic Patterning Photochemical Patterning Microcontact Printing Outlook
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A facile, maskless, digital liquid patterning method, allowing on-demand fabrication of liquid patterns without the need to change the substrate or use different photomasks, is described. Patterns with customized geometries can be created manually by simple pipetting. An application of this maskless method to create patterns of particles, cells, hydrogels, and complex liquid gradients compatible with cell studies is shown.
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Abstract The technologies of nanofabrication as applied to inorganic materials and substrates are advanced and continue to develop. These sophisticated processes enable the formation of complex electronic, optical and mechanical devices with feature sizes down to tens of nanometers. Adaptation of these types of processes to surface chemical patterning and topographical patterning provides a new set of experimental tools for investigating biological systems and realizing sensors and devices that require the interaction of biological systems and fluids with inorganic materials and surfaces. In this talk we discuss methods of pattering self assembled monolayers, proteins and antibodies on silicon and glass surfaces by lithography and microcontact printing. This is of utility in a variety of experiments in cell-surface interactions and in sensor devices. In certain types of devices the manipulation of fluids and sieving of molecules is a critical function such as in DNA sequencing6 by electrophoretic separation.
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The inside cover image is a 2D pattern of water surface-tension-confined channels formed on an array of hydrophilic spots (“digits”) surrounded by hydrophobic barriers. This method, called digital liquid patterning (DLP), allows for the formation of liquid patterns of arbitrary and easily variable geometries without the need for the fabrication of new photomasks for every pattern with a different geometry. Further details can be found in article 1300075 by P. A. Levkin and co-workers.
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