Culture and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) proven Tuberculous Endophthalmitis - First report from a Tertiary Eye Centre

目的建立一种快速灵敏特异的检测甲肝疫苗滴度的巢式RT-PCR检测方法.方法运用巢式RT-PCR法对甲肝疫苗病毒滴度进行检测,并与细胞培养ELISA检测法进行比较.结果巢式RT-PCR灵敏度和特异性均与细胞培养ELISA检测法相似.结论巢式RT-PCR是一种简便、快速、灵敏的甲肝病毒检测方法,用于疫苗的常规检测有较好前景。

Infectivity

10.3760/cma.j.issn.1003-9279.2006.04.038

Cite

Citations (0)

Polymerase Chain Reaction (PCR) in the Diagnosis of Fungal Infections of the Eye

Investigative Ophthalmology & Visual Science (2002)

R.I. Font TG Wu KR Wilhelmus B.M. Mitchell

Source

Cite

Citations (0)

Utility of Reverse Transcriptase (RT) In Situ Polymerase Chain Reaction (PCR) in the Diagnosis of Viral Infections

Journal of Histotechnology (1994)

Cheng Cheng

RNA-Directed DNA Polymerase

10.1179/014788894794710823

Cite

Citations (2)

The development of a reverse transcriptase-polymerase chain reaction (RT-PCR) for diagnosis of porcine epidemic diarrhea virus

Janet Jacob Warg

RNA-Directed DNA Polymerase

Source

Cite

Citations (0)

Occult Pulmonary Synovial Sarcoma Confirmed by Molecular Techniques

Pediatric and Developmental Pathology (2000)

Pedram Argani Frederic B. Askin Paul M. Colombani Elizabeth J. Perlman

We report a unique case of a minute, occult synovial sarcoma of the lung detected intraoperatively during a pneumothorax repair in a 17-year-old boy. No alternative primary site could be detected upon complete body imaging studies and physical examinations. The diagnosis was confirmed by demonstration of the characteristic SYT/SSX gene fusion by reverse transcriptase polymerase chain reaction (RT-PCR) performed upon RNA extracted from the paraffin block of the biopsy. This case demonstrates the utility of this technique in diagnostic pathology.

Synovial Sarcoma

Occult

10.1007/s100240050011

Cite

Citations (14)

Hepatitis C virus (HCV) in blood donors: diagnosis by the reverse transcriptase-polymerase chain reaction method (RT-PCR)

Journal of Hepatology (1998)

Neiva Sellan Lopes Gonçales C.A. Escanhola José Vassallo Fernando Lopes Gonçales Fernando Ferreira Costa

10.1016/s0168-8278(98)80919-7

Cite

Citations (0)

COMPARISON OF CONVENTIONAL BACTERIOLOGY WITH POLYMERASE CHAIN REACTION (PCR) TECHNIQUE FOR RAPID DIAGNOSIS OF TUBERCULOUS MENINGITIS

Journal of Ardabil University of Medical Sciences (2008)

Mohammad Yousef Alikhani M M Aslani H Piri Dougaheh M. Dehghan

Bacteriology

Tuberculous Meningitis

Source

Cite

Citations (0)

Polymerase Chain Reaction in the Diagnosis of Infectious Endophthalmitis

Journal of the Korean Ophthalmological Society (2001)

Sung Jin Lee You Jeong Choi Yong Sung You Hyoung Jun Koh Joon H Lee

Source

Cite

Citations (0)

The study on rapid diagnosis of infective endophthalmitis with polymerase chain reaction combined with direct gene sequencing

Beijing Medical Journal (2008)

Yi Liu

Objective To investigate the feasibility and effectivity of polymerase chain reaction associated with direct gene sequencing technique, and to find a new method for rapid diagnosis of infective endophthalmitis. Methods 47 cases diagnosed as infective endophthalmitis were included. The specimen taken in operations were detected by polymerase chain reaction associated with direct gene sequencing technique. Meanwhile nacterial smear and bacteria, fungus, anaerobic culture were detected at the same time. Results It only took 8~10 hours by polymerase chain reaction associated with direct gene sequencing technique. In 47 cases, 25 PCR detections are positive. 19 pathogens had been detected in 25 cases with direct gene sequencing technique. Conclusions The polymerase chain reaction combined with direct gene sequencing technique is a rapid and effective method for the diagnosis of infective endophthalmitis comparing with traditional pathogen detection.

Source

Cite

Citations (0)