logo
    Isolation and identification of the rare actinomycete, Amycolatopsis roodepoortensis strain EA7 from the agricultural soils of northern Iran and identification of their biological products
    Research Square (Research Square) (2024)
    Elham AmiriMirsasan MirpourKhosro IssazadehBehnam Rasti
    0
    Citation
    24
    Reference
    10
    Related Paper
    Abstract:
    Abstract This paper delves into the antimicrobial activity and identification of bioactive compounds of Amycolatopsis roodepoortensis strain EA7. Biochemical and molecular methods were utilized for the identification of actinomycetes. One strain displaying superior antimicrobial activity was chosen for the identification of bioactive compounds. The antimicrobial activity was thoroughly investigated. The analysis of the 16S rRNA gene revealed that strain EA7 belonged to the Amycolatopsis roodepoortensis specie with 99.63% confidence. The ethyl acetate extract exhibited the largest zone of inhibition against gram-positive pathogenic bacteria (25mm) using the disc diffusion method. In the MIC method, the ethyl acetate extract displayed the lowest MIC values ranging from 312.5 µg/mL (S. aureus PTCC 1112) to 1250 µg/mL (P. aeruginosa clinical and standard strain). However, the methanolic extract showed lower antimicrobial activity. In the GC-MS analysis, compounds were identified based on their percentage of area, retention time, molecular formula, molecular weight, and quality in the strain EA7 extract, with acetic acid, 2-methylpropyl ester (15.8%) being the major compound. In the LC-MS analysis, nine major compounds with anticancer and antimicrobial activity were identified. Among these, tetrangomycin, amycolactam, dihydroxybenzamide, and dipyrimycin A are compounds with potential anticancer activity, while tetracycline exhibits potential antimicrobial activity.
    Keywords:
    Identification
    Isolation
    Strain (injury)
    Topics:
    Genomics and Phylogenetic Studies
    Microbial Natural Products and Biosynthesis
    Mycorrhizal Fungi and Plant Interactions
    [CONTRIBUTION TO METHODS OF ISOLATION OF C1. PERFRINGENS. III. ISOLATION OF CULTURES AT 46-47 DEGREES C].
    PubMed (1963)
    A SăsármanT Horodniceanu
    Isolation
    Citations (0)
    Prevention of Infection in Wards (2) Isolation of Patients
    Springer eBooks (1998)
    E. J. L. LowburyG.A.J. AyliffeA. M. GeddesJ. D. Williams
    Isolation
    Patient isolation
    Infection Risk
    Citations (2)
    Notes on the isolation and identification of picromycin
    Československá Mikrobiologie (1958)
    З. ВанекLibuše Dolezilov↠Z. Řeháček
    Isolation
    Identification
    Citations (2)
    [Public health and isolation measures with regard to infectious patients].
    PubMed (1991)
    Widmer Hr
    Patient isolation aims at reducing the risk of infection transmission to other patients and the hospital personnel. Three types of disease-specific isolation procedures and their indications are described: strict isolation, respiratory isolation and enteral isolation. The type of patient isolation to be applied depends on the mode of pathogen transmission and on the severity of the disease it produces.
    Isolation
    Patient isolation
    Citations (0)
    The Role of Agricultural Cooperatives in the Development of Regional Agriculture
    Journal of Rural Problems (1991)
    Kazuo Tamonin
    The Japanese agriculture has been deeply damaged by an increase of imported agricultural products. In the course of in creasing import, main agricultural regions in Japan has been suffered. Under the circumstance, agricultural cooperatives are expected to promote regional agriculture and to sustain regional economy. In the paper, case studies show how agricultural cooperatives promote regional agriculture.
    Citations (0)
    CONSTRUCT ECO-AGRICULTURE AND PRODUCE LESS HARMFUL AGRICULTURE PRODUCTS TO ENHANCE THE COMPETITIVENESS OF JIANGSU AGRICULTURE
    Chinese Journal of Population Resources and Environment (2002)
    Li Rong
    Green food
    Ecological agriculture
    Citations (0)
    Cost of isolation: Daily cost of isolation determined and cost avoidance demonstrated from the overuse of personal protective equipment in an acute care facility
    American Journal of Infection Control (2014)
    Kerrie VerLeeDorine Berriel‐CassKristen BuckChàu Nguyen
    Isolation
    Personal Protective Equipment
    Patient isolation
    Citations (30)
    Outpatient Isolation Solution
    American Journal of Infection Control (2011)
    Kim NguyenC Perego
    Isolation
    Personal Protective Equipment
    Patient isolation
    Citations (0)
    Burden of isolation for multidrug-resistant organisms in a tertiary public hospital in Southern Brazil
    American Journal of Infection Control (2014)
    Patrícia M. R. PereiraPetros IsaakidisSven Gudmund HinderakerEngy AliWilma van den Boogaard
    Isolation
    Carbapenem-resistant enterobacteriaceae
    Patient isolation
    Gram-negative bacterial infections
    Citations (6)
    On the Roles of Local High Agricultural Universities and Colleges in Educated Agriculture
    Shanxi nongye daxue xuebao. Ziran kexue ban (2005)
    Yue Wen-bing
    The twenty-first century is an educated agricultural time.This paper begins with analyzing the distinguishing features of educated agriculture and its requirements of the high agricultural universities and colleges,and then sums up the experiences of the services of ShanXi Agricultural University to the educated agriculture.Moreover,combining the reality of Shanxi,the paper suggests that the educated agriculture should be research projects in these fields of breeding animals and plants,drying farming as water-saving,biological engineering,ecological environments,agriculture of facilities and so on,and proposes five ways of doing the job well.
    Agricultural Education
    Citations (0)