logo
    Xenogenous implanted dental follicle stem cells promote periodontal regeneration throughinduces N2 phenotype of neutrophils
    Research Square (Research Square) (2024)
    Li LiuYuqi WenLiangrui ChenLi Mao-xueJialu YuWeidong TianYafei WuShujuan Guo
    0
    Citation
    59
    Reference
    10
    Related Paper
    Abstract:
    Abstract Background Periodontal tissue loss is the main reason of tooth mobility and loss caused by periodontal disease. Dental follicle stem cells (DFSCs) have significant therapeutic potential in periodontal regeneration, which maybe mainly depends on its potent immunomodulatory capacity. Consequently, this study aims to elucidate the impact of implanted xenogenous DFSCs on innate immune responses during early and late stage in periodontal defect repair period. Methods To trace and investigate the immunomodulation mechanisms of DFSCs in vivo, DFSCs were engineered (E-DFSCs) using lentiviral vectors expressing CD63-enhanced green fluorescent protein (CD63-EGFP) and β-Actin-mCherry protein (ACTB-mCherry) to exhibit green and red fluorescence. The biological characteristics and functions of E-DFSCs were verified by proliferation, differentiation and co-culture experiments in vitro. In vivo, the periodontal regeneration capacity of E-DFSC was detected by implantation of murine periodontal defect model, and the response of innate immune cells were detected at 1st ,3rd, 5th day (early stage) and 4th week (late stage) after implantation. Results In vitro assessments showed that E-DFSCs retain similar properties to their non-engineered counterparts but exhibit enhanced macrophage immunomodulation capability. In mice models, four-week micro-CT and histological evaluations indicated that E-DFSCs have equivalent efficiency to DFSCs in periodontal defects regeneration. At the early stage of repair in mice periodontal defect, fluorescence tracking showed that implanted E-DFSCs might primarily activate endogenous cells through direct contact and indirect actions, and most of these cells is myeloperoxidase positive neutrophils. Additionally, compared with the control group, the neutrophils infiltration and conversion of N2-type were significantly increased in the E-DFSC group. At the late stage of defect regeneration, more M2-type macrophages, fewer TRAP + osteoclasts and upregulated OPG/RANKL ratio were detected in the E-DFSC group compared to the control group, which indicated that immune balance tilt toward healing and bone-formation. Conclusion The xenogenous implanted DFSCs can induce N2 phenotype of neutrophils in early stage, which can activate the innate immune mechanism of the host to promote periodontal tissue regeneration.
    Keywords:
    Dental follicle
    Topics:
    Neutrophil, Myeloperoxidase and Oxidative Mechanisms
    Mesenchymal stem cell research
    Oral microbiology and periodontitis research
    Dental Tissue — New Source for Stem Cells
    The Scientific World JOURNAL (2009)
    Vladimír PetrovičVladisav Stefanović
    Stem cells have been isolated from many tissues and organs, including dental tissue. Five types of dental stem cells have been established: dental pulp stem cells, stem cells from exfoliated deciduous teeth, stem cells from apical papilla, periodontal ligament stem cells, and dental follicle progenitor cells. The main characteristics of dental stem cells are their potential for multilineage differentiation and self-renewal capacity. Dental stem cells can differentiate into odontoblasts, adipocytes, neuronal-like cells, glial cells, osteoblasts, chondrocytes, melanocytes, myotubes, and endothelial cells. Possible application of these cells in various fields of medicine makes them good candidates for future research as a new, powerful tool for therapy. Although the possible use of these cells in therapeutic purposes and tooth tissue engineering is still in the beginning stages, the results are promising. The efforts made in the research of dental stem cells have clarified many mechanisms underlying the biological processes in which these cells are involved. This review will focus on the new findings in the field of dental stem cell research and on their potential use in the therapy of various disorders.
    Dental follicle
    Dental papilla
    Citations (42)
    Application of dental stem cells in three-dimensional tissue regeneration
    World Journal of Stem Cells (2021)
    Hui‐Yi HsiaoChung‐Yi NienHsiang‐Hsi HongMing‐Huei ChengTzung‐Hai Yen
    Dental stem cells can differentiate into different types of cells. Dental pulp stem cells, stem cells from human exfoliated deciduous teeth, periodontal ligament stem cells, stem cells from apical papilla, and dental follicle progenitor cells are five different types of dental stem cells that have been identified during different stages of tooth development. The availability of dental stem cells from discarded or removed teeth makes them promising candidates for tissue engineering. In recent years, three-dimensional (3D) tissue scaffolds have been used to reconstruct and restore different anatomical defects. With rapid advances in 3D tissue engineering, dental stem cells have been used in the regeneration of 3D engineered tissue. This review presents an overview of different types of dental stem cells used in 3D tissue regeneration, which are currently the most common type of stem cells used to treat human tissue conditions.
    Dental follicle
    Dental papilla
    Amniotic stem cells
    Citations (8)
    Potential Use of Dental Stem Cells for Craniofacial Tissue Regeneration
    Stem cell biology and regenerative medicine (2013)
    Mustafa RamazanoğluKarl Andreas SchlegelGamze Torun Köse
    Dental follicle
    Citations (3)
    Dental stem cells and their application in dentistry.
    Journal Of Oral Research (2020)
    Heber Arbildo‐VegaFredy Hugo Cruzado-OlivaEdward Infantes
    Recent advances in tissue engineering and regenerative medicine offer a long-term solution through biological repair, replacement of damaged teeth or maintenance and improvement of tissue and organ function through the use of stem cells. Stem cells or also called universal cells, progenitor cells or precursor cells; they are primitive, undifferentiated, clonogenic cells that are characterized by their self-renewal capabilities and that can be differentiated into more specialized cells with specific functions. Currently many sources are known from where you can obtain stem cells, one of which are those obtained from oral or dental tissues, called dental stem cells (DSC), from where it has been possible to identify, isolate and characterize around 8 unique populations: dental pulp stem cells (DPSC), human exfoliated deciduous tooth stem cells (SHED), periodontal ligament stem cells (PLDSC), dental follicle stem cells (DFSC), stem cells derived from bone alveolar (CMHA), the stem cells of the apical papilla (SCAP), the stem cells of the dental germ (DGSC) and the gingival stem cells (GSC). These DSC have attracted attention in recent years due to their accessibility, plasticity and high proliferation capacity. Currently, DSC have shown that they can be used in endodontic and periodontal regenerative therapy, in the regeneration of dentin and bone and in dental bioengineering. Tissue engineering methodologies combined with a greater understanding of the biology of DSCs will provide powerful tools for a broader spectrum of their application in various future therapeutic strategies.
    Dental follicle
    Dental papilla
    Regenerative Medicine
    Citations (3)
    DENTAL STEM CELLS (CONCEPTS AND APPLICATIONS)
    Alexandria Dental Journal (2020)
    Ahmed HusseinZeinab DarwishHanaa RaslanMahmoud AttiaHamida Abdel‐Hamid
    Stem cells constitute the source of differentiated cells for the generation of tissues during development, as well as for regeneration of tissues that are diseased or injured postnatally. The stem cell research has grown exponentially to improve the life of patients with conditions that span from Alzheimer's disease, cardiac ischemia to bone or tooth loss. In dentistry, stem cell biology and tissue engineering are of great interest since they may provide an innovative for generation of clinical material and/or tissue regeneration. Mesenchymal stem cells were demonstrated in dental tissues, including dental pulp, periodontal ligament, dental papilla, and dental follicle. These stem cells can be isolated and grown under defined tissue culture conditions and are potential cells for use in tissue engineering including dental tissue, nerves and bone regeneration under appropriate conditions. This review was performed to study the concepts of stem cells and the most recent application of dental stem cells.
    Dental follicle
    Dental papilla
    Amniotic stem cells
    Periodontal fiber
    Citations (1)
    Stem Cells in Dentistry
    BENTHAM SCIENCE PUBLISHERS eBooks (2010)
    Li Wu ZhengLim Kwong Cheung
    Stem cells have been isolated and characterized from embryonic, fetal, and adult tissues. The therapeutic and clinical application of embryonic stem cells and fetal stem cells is challenging to the many ethical and political controversies concerning their use. Adult stem cells have been isolated and characterized from a wide variety of tissues including bone marrow, brain, skin, hair follicles, skeletal muscle, adipose tissue, cord blood, dental tissue, and their differentiation potential may reflect their local environment. To date, several sources of dental stem cells have been isolated and being characterized as dental epithelial stem cells, dental pulp stem cells, dental follicle precursor cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla, and periodontal ligament stem cellsDental stem cells have been shown to have multipotential by their ability to differentiate into neuronal, adipogenic, myogenic, chondrogenic, osteogenic and dentinogenic cells when cultured under specific conditions. These facilitated studies to address an important property of stem cells, that is, the capacity of a given stem cell population to regenerate an organized, functional tissue following transplantation in vivo. Furthermore, the ready availability of tooth tissues from redundant teeth such as third molars can provide a good supply of dental stem cells that may be utilized for regenerating other body parts or organs.
    Dental follicle
    Amniotic stem cells
    Amniotic epithelial cells
    Citations (1)
    Stem Cells in Paediatric Dentistry
    LAP LAMBERT Academic Publishing eBooks (2018)
    Gundeep MadanNidhi Agarwal
    In humans, there are two broad types of stem cells- Embryonic stem cells and Adult stem cells. Adult stem cell population has been found in many tissues of human body eg. Bone marrow,adipose derived, dental stem cells etc. Dental Stem Cells can be Dental Pulp Stem Cells (DPSC), Stem Cells from Human Exfoliated Deciduous teeth (SHED), Periodontal Ligament Stem Cells (PDLSC), Stem Cells from Apical Papilla ( SCAP), Dental Follicular Stem Cells (DFSC) these stem cells can be isolated and stored for its use in future. They have an important role in future of medicine and dentistry.
    Dental follicle
    Amniotic stem cells
    Dental papilla
    Amniotic epithelial cells
    Citations (0)
    Stand der humanen dentalen Stammzellforschung
    Mund- Kiefer- und Gesichtschirurgie (2007)
    Christian MorsczeckTorsten E. ReichertFlorian VöllnerTill GerlachOliver Driemel
    Dental follicle
    Citations (14)
    Cells isolated from cryopreserved dental follicle display similar characteristics to cryopreserved dental follicle cells
    Cryobiology (2017)
    Hefeng YangJie LiJingjing SunWeihua GuoHui Li
    Dental follicle
    Citations (9)
    Dental stem cells--characteristics and potential.
    PubMed (2014)
    Sanja BojićVladislav VolarevićBiljana LjujićMiodrag Stojković
    Soft dental tissues have been identified as easily accessible sources of multipotent postnatal stem cells. Dental stem cells are mesenchymal stem cells (MSC) capable of differentiating into at least three distinct cell lineages: osteo/odontogenic, adipogenic and neurogenic. They express various markers including those specific for MSC, embryonic stem cells and neural cells. Five different types of dental stem cells have been isolated from mature and immature teeth: dental pulp stem cells, stem cells from exfoliated deciduous teeth, periodontal ligament stem cells, stem cells from apical papilla and dental follicle progenitor cells. Dental stem cells may be used in dental tissue engineering including dental, enamel and periodontal tissue regeneration. They could also be used as a promising tool in potential treatment of neurodegenerative, ischemic and immune diseases.
    Dental follicle
    Dental papilla
    Amniotic stem cells
    Multipotent Stem Cell
    Amniotic epithelial cells
    Citations (63)