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    Lactate-Related Gene Signatures as Prognostic Predictors and Comprehensive Analysis of Immune Profiles in Nasopharyngeal Carcinoma
    Changlin LiuChuping NiChao LiTian HuW. W. JianYuping ZhongYanqing ZhouXiaoming LyuYuanbin ZhangXiaojun XiangChao ChengXin Li
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    Abstract:
    Abstract Objectives Nasopharyngeal carcinoma (NPC) is an aggressive malignancy with high rates of morbidity and mortality, largely because of its late diagnosis and metastatic potential. Lactate metabolism and protein lactylation are thought to play roles in NPC pathogenesis by modulating the tumor microenvironment and immune evasion. However, research specifically linking lactate-related mechanisms to NPC remains limited. This study aimed to identify lactate-associated biomarkers in NPC and explore their underlying mechanisms, with a particular focus on immune modulation and tumor progression. Methods To achieve these objectives, we utilized a bioinformatics approach in which publicly available gene expression datasets related to NPC were analysed. Differential expression analysis revealed differentially expressed genes (DEGs) between NPC and normal tissues. We performed weighted gene coexpression network analysis (WGCNA) to identify module genes significantly associated with NPC. Overlaps among DEGs, key module genes, and lactate-related genes (LRGs) were analysed to derive lactate-related differentially expressed genes (LR-DEGs). Machine learning algorithms can be used to predict potential biomarkers, and immune infiltration analysis can be used to examine the relationships between identified biomarkers and immune cell types, particularly M0 macrophages and B cells. Results A total of 1,058 DEGs were identified between the NPC and normal tissue groups. From this set, 372 key module genes associated with NPC were isolated. By intersecting the DEGs, key module genes, and lactate-related genes (LRGs), 17 lactate-related DEGs (LR-DEGs) were identified. Using three machine learning algorithms, this list was further refined, resulting in three primary lactate-related biomarkers: TPPP3, MUC4, and CLIC6. These biomarkers were significantly enriched in pathways related to "immune cell activation" and the "extracellular matrix environment." Additionally, M0 and B macrophages were found to be closely associated with these biomarkers, suggesting their involvement in shaping the NPC immune microenvironment. Conclusion In summary, this study identified TPPP3, MUC4, and CLIC6 as lactate-associated clinical modelling indicators linked to NPC. linked to NPC, providing a foundation for advancing diagnostic and therapeutic strategies for this malignancy.
    Topics:
    RNA modifications and cancer
    Cancer-related molecular mechanisms research
    Cancer Immunotherapy and Biomarkers
    Sequence Analysis of EBV-BARFO Gene in Nasopharyngeal Carcinoma from Guangdong Area
    MA Ying-h
    Objective:There were variations of EBV genome f rom different areas and they may affe ct the biologic function of EBV(such as LMP1).EBV-BARF0was highly detected in th e patients with nasopharyngeal carc inoma (NPC)and its variations have not been rep orted.This study was designed to determine the sequence and variation of EBV-BARF0gene in the patieats with NPC from Guangdong area.Methods :PCR was used to amplify the EBV-BARF0gene in 20patients with NPC and the production s were sequenced on the ABI377.Results:Comparing with standard B95-8,there were four loci with variances i ncluding:160473(G→T),160545(C→T),160701(C→A),160707(G→C)of sequences and 2(Ala→Ser),26(Leu→Phe),78(Arg→Ser),80(Ala→Pro)of amino acid in 20patients with NPC.Conclusion:Since the BARF0gene of EBV is expressed in all nasopharyngeal carcinoma c ell line and biopsies,but it is not expressed or less frequenly in lymphoma tissues and cell lines,the authors firstly reported the variation of EBV-BARF0which compared with B95-8in the patieats with NPC from Guangdong area.These su ggest that the gene may play an important role in the carcinogenesis and development of nasopharyngeal carcinoma .
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    Data from Multiparametric Detection of Antibodies against Different EBV Antigens to Predict Risk for Nasopharyngeal Carcinoma in a High-Risk Population of China
    Hao ChenShulin ChenJie LuXueping WangJianpei Li
    <div>Abstract<p>In this study, we aimed to use the combined detection of multiple antibodies against Epstein–Barr virus (EBV) antigens to develop a model for screening and diagnosis of nasopharyngeal carcinoma (NPC). Samples of 300 nasopharyngeal carcinoma patients and 494 controls, including 294 healthy subjects (HC), 99 non-nasopharyngeal carcinoma cancer patients (NNPC), and 101 patients with benign nasopharyngeal lesions (BNL), were incubated with the EUROLINE Anti-EBV Profile 2, and band intensities were used to establish a risk prediction model. The nasopharyngeal carcinoma risk probability analysis based on the panel of VCAgp125 IgA, EBNA-1 IgA, EA-D IgA, EBNA-1 IgG, EAD IgG, and VCAp19 IgG displayed the best performance. When using 26.1% as the cutoff point in ROC analysis, the AUC value and sensitivity/specificity were 0.951 and 90.7%/86.2%, respectively, in nasopharyngeal carcinoma and all controls. In nasopharyngeal carcinoma and controls without the non-nasopharyngeal carcinoma and BNL groups, the AUC value and sensitivity/specificity were 0.957 and 90.7%/88.1%, respectively. The diagnostic specificity and sensitivity of the EUROLINE Anti-EBV Profile 2 assay for both nasopharyngeal carcinoma and early-stage nasopharyngeal carcinoma were higher than that of mono-antibody detection by immune-enzymatic assay and real-time PCR (EBV DNA). In the VCA-IgA–negative group, 82.6% of nasopharyngeal carcinoma patients showed high probability for nasopharyngeal carcinoma, and the negative predictive value was 97.1%. In the VCA-IgA–positive group, 73.3% of healthy subjects showed low probability. The positive predictive value reached 98.2% in this group. The nasopharyngeal carcinoma risk probability value determined by the EUROLINE Anti-EBV Profile 2 might be a suitable tool for nasopharyngeal carcinoma screening. <i>Cancer Prev Res; 10(9); 542–50. ©2017 AACR</i>.</p></div>
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    Analysis of MRI in Secretory Otitis Media in Patient with Nasopharyngeal Carcinoma
    Hebei yixue (2008)
    Yanxia Huang
    Objective:To explore the usefulness of MRI in nasopharyngeal carcinoma(NPC) with seretory otitis media(SOM).Method: MRI of the nasopharynx and matoid in 130 cases with NPC pro and postirrdiation were analyzed.Result:Total incidence rate of SOM was 42% after irradiation.Conclusion:MRI for the patient with NPC is useful for the diagnosis of SOM induced by NPC.
    Acute Otitis Media
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    Data from Multiparametric Detection of Antibodies against Different EBV Antigens to Predict Risk for Nasopharyngeal Carcinoma in a High-Risk Population of China
    Hao ChenShulin ChenJie LuXueping WangJianpei Li
    <div>Abstract<p>In this study, we aimed to use the combined detection of multiple antibodies against Epstein–Barr virus (EBV) antigens to develop a model for screening and diagnosis of nasopharyngeal carcinoma (NPC). Samples of 300 nasopharyngeal carcinoma patients and 494 controls, including 294 healthy subjects (HC), 99 non-nasopharyngeal carcinoma cancer patients (NNPC), and 101 patients with benign nasopharyngeal lesions (BNL), were incubated with the EUROLINE Anti-EBV Profile 2, and band intensities were used to establish a risk prediction model. The nasopharyngeal carcinoma risk probability analysis based on the panel of VCAgp125 IgA, EBNA-1 IgA, EA-D IgA, EBNA-1 IgG, EAD IgG, and VCAp19 IgG displayed the best performance. When using 26.1% as the cutoff point in ROC analysis, the AUC value and sensitivity/specificity were 0.951 and 90.7%/86.2%, respectively, in nasopharyngeal carcinoma and all controls. In nasopharyngeal carcinoma and controls without the non-nasopharyngeal carcinoma and BNL groups, the AUC value and sensitivity/specificity were 0.957 and 90.7%/88.1%, respectively. The diagnostic specificity and sensitivity of the EUROLINE Anti-EBV Profile 2 assay for both nasopharyngeal carcinoma and early-stage nasopharyngeal carcinoma were higher than that of mono-antibody detection by immune-enzymatic assay and real-time PCR (EBV DNA). In the VCA-IgA–negative group, 82.6% of nasopharyngeal carcinoma patients showed high probability for nasopharyngeal carcinoma, and the negative predictive value was 97.1%. In the VCA-IgA–positive group, 73.3% of healthy subjects showed low probability. The positive predictive value reached 98.2% in this group. The nasopharyngeal carcinoma risk probability value determined by the EUROLINE Anti-EBV Profile 2 might be a suitable tool for nasopharyngeal carcinoma screening. <i>Cancer Prev Res; 10(9); 542–50. ©2017 AACR</i>.</p></div>
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    Link of Nasopharyngeal Carcinoma and Epstein-Barr Virus
    Biology Medicine & Natural Product Chemistry (2018)
    Sugiyanto SugiyantoLina AryatiFajar Adi-KusumoMardiah Suci Hardianti
    Nasopharyngeal Carcinoma (NPC) is a cancer that occurs in nasopharynx which is associated with Epstein-Barr Virus (EBV). Mutation agents in nasopharyngeal neoplasms occur because of EBV infection. Transformation of B-cells due to EBV causes hormone imbalance in lymphoid cells or nasopharyngeal epithelial tissue. Rates of EBV infection have been shown to be prognostic to NPC. The basic level of EBV DNA can be used for stratification prognosis, with higher titers showing greater disease severity and worse outcomes. With mathematical models, there is a correlation between the increase in Epstein-Barr Virus and the increase in Invasive Carcinoma Cells or increase in Nasopharyngeal Carcinoma Cells.
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    Correlation between Epstern-Barr virus andangiogenesis of patients with nasopharyngeal carcinoma
    Jia Li
    Objective To explore correlation between Epstern-Barr virus and angiogenesis of patients with nasopharyngeal carcinoma.Methods Epstern-Barr virus DNA of 54 patients with nasopharyngeal carcinoma was measured by PCR and their serum vascular endothelial growth factor(serum VEGF) level was also detected by ELISA,and these data was compared and analyzed with serum vascular endothelial growth factor level of 40 health control.Results Serum vascular endothelial growth factor level of 54 patients with nasopharyngeal carcinoma(143.86±19.49) pg/mL was very higher than that of 40 health control(51.23±12.66) pg/mL,(P0.01);Serum vascular endothelial growth factor level of 24 EBV positive patients with nasopharyngeal carcinoma(195.44±19.69) pg/mL was remarkable higher than that of 30 EBV negative patients with nasopharyngeal carcinoma(154.58±15.23 pg/mL),(P0.01).Conclusion Serum vascular endothelial growth factor level changed in patients with nasopharyngeal carcinoma may be as a new tumor mark for determining disease advancing about nasopharyngeal carcinoma.
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    Value Analysis of Plasma Epstein-barr Virus DNA Concentration Detection in Screening and Clinical Staging Diagnosis of Nasopharyngeal Carcinoma
    Investigación Clínica (2020)
    Yibo Yang
    Nasopharyngeal carcinoma (nasopharyngeal carcinoma) is one of the most common tumor diseases at present. Due to its special clinicopathological characteristics and strong sensitivity to radiotherapy, it is easy to relapse and even cause distant metastasis after treatment. At present, the screening and diagnosis of nasopharyngeal carcinoma mainly relies on image detection. This detection method shows low sensitivity in the screening and clinical staging of nasopharyngeal carcinoma, so the effect is not ideal. The purpose of this paper is to study the significance of epstein-barr virus DNA concentration detection in the screening of nasopharyngeal carcinoma and clinical staging diagnosis, so as to provide theoretical support for the diagnosis of nasopharyngeal carcinoma. This article first to EB virus, and discusses the specific concept of nasopharyngeal carcinoma (NPC), then simply discusses the EB virus and development of the relationship between nasopharyngeal carcinoma, and the studies of the relationship between the simple illustration, finally analyzed with related experiments EB virus DNA testing in the value of screening and clinical staging diagnosis of nasopharyngeal carcinoma (NPC). The experimental results showed that the basic age composition of the three groups of people in the study was relatively consistent. The average age was 49.6 years old, and the majority of them were males, accounting for 85.4% of the total number of patients. The positive values of vca-iga antibody and epstein-barr virus DNA in different groups were the highest in the nasopharyngeal carcinoma group, with concentrations of 91.6% and 52.1%, respectively. Compared with the traditional detection methods, the detection of EB virus DNA concentration has been significantly improved in the diagnosis speed and effect of nasopharyngeal carcinoma, with the diagnosis speed increased by about 17% and the diagnosis efficiency increased by about 30%.
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    Quantitative detection of epstein-Barr virus infectious status in nasopharyngeal carcinoma tissues
    Zhonghua zhongliu fangzhi zazhi (2010)
    HU Wan-ning
    OBJECTIVE:To explore the clinical significance of quantitative detection of Epstein-Barr virus infectious status in nasopharyngeal carcinoma tissues.METHODS:The carcinoma tissues and adjacent tissues from 30 patients with poorly differentiated nasopharyngeal squamous cell carcinoma were prepared simultaneously,and nasopharyngeal tissues from 15 healthy subjects were used as the control.The EBV DNA copies in the samples were tested by using rea1-time quantitative polymerase chain reaction(PCR).RESULTS:The infectiou rates of EBV were 73.3%(11/15)in nasopharyngeal tissues of healthy subjects(mean EBV DNA copies of 6.5×102 μg-1 DNA),100%(30/30)in carcinoma tissues and 76.7%(23/30)in adjacent tissues of nasopharyngeal carcinoma patients(mean EBV DNA copies of 6.7×105 μg-1 DNA and 1.3×105 μg-1 DNA)respectively.There was no significant diference in the infectious rates and levels of EBV between the adjacent carcinoma tissues of nasopharyngeal carcinoma patients and nasopharyngeal tissues of healthy subjects(P0.05).The infectious level of EBV was significantly higher in carcinoma tissues than in adjacent tissues,and significantly higher in adjacent tissues than in normal nasopharyngeal tissues of nasopharyngeal carcinoma patients(P0.01).CONCLUSIONS:EBV latent infection is a common phenomenon in nasopharyngeal tissues of both nasopharyngeal carcinoma patients and healthy persons.The enhancement of EBV infection plays an important role during carcinogenesis of nasopharyngeal carcinoma patients,which sheds further light on the occurrence of EBV with nasopharyngeal carcinoma.
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    Characteristic miRNAs expression profile of nasopharyngeal carcinoma
    Journal of clinical and experimental medicine (2013)
    Wei Ming
    Objective To study the expression profiles of microRNA(miRNA) in nasopharyngeal carcinoma by microarray technique and explore the potential molecular signature specific for nasopharyngeal carcinoma.Methods Tissues were obtained from 8 cases of nasopharyngeal non-keratinizing carcinoma and 8 cases of chronic nasopharyngeal inflammation.Total RNA was extracted by Trizol method and hybridized with human 723 miRNA microarrays.The miRNA expression profiles of nasopharyngeal carcinoma and chronic inflammation was analyzed and differential miRNA expression between nasopharyngeal carcinomas and chronic inflammation was determined.Results miRNA expression profiling was performed to screen the miRNA expression change of the nasopharyngeal carcinoma.Thirty-one miRNAs were identified to be differentially expressed,in which 6 were overexpressed and 25 were underexpressed in nasopharyngeal carcinoma.Conclusion Thirty-one differentially expressed miRNAs were detected in nasopharyngeal carcinoma by microarray method.The study of the differentially expressed miRNAs in nasopharyngeal carcinoma may play a foundation for the advanced studies to explore the carcinogenesis,development and early diagnosis of the nasopharyngeal carcinoma.
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    Expression of Epstein-Barr virus-encoded BamH1-A rightward transcript 7 microRNA in nasopharyngeal carcinoma cells modulates the responsive to irradiation treatment
    WK Ho
    had higher sensitivity to irradiation treatment. Conclusion Our results suggest that patients with nasopharyngeal carcinoma have elevated ebv-miR-BART7 expression levels in the primary and recurrent nasopharyngeal carcinoma tissues. Higher expression of ebv-miRBART7 increased the sensitivity of nasopharyngeal carcinoma cells to irradiation treatment. Further investigation with regard to the clinical value of ebv-miR-BART7 to aid therapeutic response is warranted in order to evaluate the potential use of ebv-miR-BART7 as a molecular biomarker in monitoring patients with nasopharyngeal carcinoma.
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