Surgical Excision of an Extratesticular Anaplastic Carcinoma in a Variable Kingsnake (Lampropeltis mexicana)
0
Citation
27
Reference
10
Related Paper
Abstract:
An adult male variable kingsnake (Lampropeltis mexicana) was presented for examination due to a three-week history of anorexia and obvious body deformities. On objective examination the animal was in poor condition, and on palpation, an intracoelomic mass was noted approximately in the distal third of the body, cranial to the cloaca. In agreement with the owner, an exploratory celiotomy was planned and performed and the mass was surgically removed. Modified Wright–Giemsa stain impression smears were taken, which were consistent with an undifferentiated tumour. Histological examination revealed the presence of a solid proliferation composed of highly tubular anaplastic cells and abundant multinucleated cells. The neoplastic cells were positive for cytokeratin (AE1/AE3), but not for vimentin. Periodic acid–Schiff (PAS) staining revealed the presence of large granular cells, which can be identified as the characteristic cells of the efferent ducts. Based on the morphological and immunohistochemical findings, the diagnosis of extratesticular anaplastic carcinoma was made. To the authors’ knowledge, this type of neoplasm has never been reported in the male genital apparatus of snakes.Keywords:
Anaplastic carcinoma
Stain
Histology
Abstract An immunohistological study of 15 chordomas, six chondrosarcomas, four liposarcomas and seven carcinomas on paraffin embedded samples using anti‐cytokeratin, anti‐epithelial membrane antigen (EMA), anti‐S100 protein, anti‐vimentin and anti‐neurofilaments showed that chordomas has a characteristic immuno‐staining, i.e. positive for cytokeratin, EMA, S100 protein and vimentin; and negative for neurofilaments. This immuno‐staining allows a clear distinction of chordomas from other tumours.
Neurofilament
S100 protein
Positive staining
Cite
Citations (46)
Cite
Citations (14)
Cite
Citations (141)
Cite
Citations (105)
Immunogold labelling
Cite
Citations (5)
Co-expression of cytokeratin and vimentin has been traditionally associated with a few select tumors. However, this phenomenon is being recognized in a wider range of tumors. Twenty-one canine primary pulmonary epithelial neoplasms were evaluated for the co-expression of cytokeratin and vimentin. The histologic pattern and grade, and an immunohistochemical grade for cytokeratin and vimentin staining, were determined for each neoplasm. Adenocarcinomas predominated, and histologically, most tumors were grade II. All of the neoplasms stained positive for cytokeratin, while only 8 (38%) stained positive for both vimentin and cytokeratin. Papillary adenocarcinomas were consistently vimentin negative. The anaplastic histologic pattern had significantly more vimentin staining than the other histologic patterns. There was no significant difference in histologic grade or grading criteria between those tumors that stained with vimentin and those that did not. The present study established that cytokeratin and vimentin co-expression occurs in canine primary pulmonary epithelial tumors at a similar frequency to human pulmonary neoplasms. Further investigation will be needed to characterize the significance of this finding, particularly with respect to prognosis.
Keratin 7
Cite
Citations (23)
The reported incidence of cytokeratin and vimentin intermediate filament coexpression is rapidly expanding. An up-do-date list of the instances in which this occurs is presented and the implications of this event are discussed.
Intermediate Filament Protein
Cite
Citations (23)
Neurofilament
Cite
Citations (15)
Abstract This paper identifies another neoplasm of epithelial origin which may express vimentin in addition to cytokeratins, thereby adding to the expanding list of tumours which demonstrate intermediate filaments (IFs) other than those of their reputed cell of origin. Twenty examples of benign breast disease and 66 carcinomas were examined for vimentin and cytokeratin IFs using an avidin–biotin–peroxidase complex technique. Co‐expression of these IF proteins was found in 35 per cent of cases of benign breast tissue and in 60 per cent of the carcinomas. In 8 (16 per cent) of 50 cases of infiltrating ductal carcinoma, vimentin and cytokeratin immunostaining was observed in more than 60 per cent of the tumour cells. These carcinomas were predominantly of a high histological grade. In benign breast disease and well‐differentiated carcinoma, vimentin was distributed in the basal and perinuclear regions of the cells, with sparing of the apical portions. In those cases in which large numbers of tumour cells expressed vimentin, cytoplasmic staining was diffuse, and often exhibited distinctive perinuclear and subplasmalemmal accentuation. We propose that a knowledge of the list of carcinomas which may co‐express vimentin and cytokeratin IFs might be helpful in the assessment of undifferentiated tumours and metastatic deposits.
Immunostaining
Breast carcinoma
Intermediate Filament Protein
Keratin 8
Metastatic carcinoma
Cite
Citations (93)