Heterogeneous nucleation induced A. pernyi/B. mori silk fibroin coatings on AZ31 biometals with enhanced corrosion resistance, adhesion and biocompatibility
Xinru CaoYanning ChenChen ZhangZhinan MaoJingwu ZhangTingji MaWenhan TianKong XiangshengHaotong LiSixian RaoKang Yang
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Keywords:
Fibroin
Biocompatibility
Biomaterial
Antheraea pernyi
Investigations have been made on the molecular conformation in the solid state of Bombyx mori silk fibroin and the model polypeptides by an infrared spectroscopy (IR).An IR absorbed band assigned to silk I type conformation was only found for the precipitates not having any random coil obtained by an enzyme treatment to the middle silk gland diluted with water and by dissolving silk fibers in an aqueous LiBr solution. On the other hand, the IR absorbed band assigned to α-helical conformation was found together with those of random coil and the silk II type conformation for as-polymerized model polypeptides and silk fibroin threads hydrolyzed by aqueous HCI solution.It is, therefore, concluded that a small amount of α-helix exists in the amorphous part of Bombyx mori silk fibroin.
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Random coil
Bombycidae
Helix (gastropod)
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The regenerated Antheraea pernyi silk fibroin (RASF) was harvested by dissolving the silk fibers in a calcium nitrate solution. XRD result demonstrated that both native and regenerated Antheraea pernyi silk fibroin involved the α-helix conformation, and DTG curves showed that their thermal decompositions were quite similar and proceeded three steps. However, rheological measurements figured out that the molecule weight of RASF decreased from 246 kDa to 199 kDa, comparing with native Antheraea pernyi silk fibroin. Also, the tensile properties of the RASF film, which were the same to those of regenerated Bombyx mori silk fibroin film, were observed.
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众多研究表明,含有三肽基序即精氨酸-甘氨酸-天冬氨酸(RGD)的材料有助于细胞在其上的粘附和生长.尽管作为天然蛋白质的柞蚕丝蛋白同样具有RGD序列,但其从柞蚕丝纤维中的再生过程复杂且不易加工成型,因此对此类蛋白质材料的研究较少.我们以水溶液共混的方式,在再生柞蚕丝蛋白中引入了更为常用的桑蚕丝蛋白,通过对桑蚕丝蛋白水溶液特殊有效的醇诱导和冻融方法,成功制备了非水溶性的柞蚕丝蛋白/桑蚕丝蛋白复合支架材料.研究结果显示,随着柞蚕丝蛋白含量增加,共混支架的孔径和强度均有所下降,但其热稳定性上升;FTIR测试表明,尽管柞蚕丝蛋白和桑蚕丝蛋白的一级结构完全不同,但其二级结构在以此方法制备的支架材料中均以β-折叠构象为主.体外细胞培养实验证实,小鼠成纤维细胞和成骨细胞都能在支架上增殖生长,并且共混支架较纯桑蚕丝蛋白支架具有更高的细胞增殖速率,表明其不仅具有良好的生物相容性,而且可能更适用于组织工程领域.
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The mRNA that codes for silk fibroin in the silkworm Bombyx mori can be isolated in greater than 90% purity as judged by partial sequence analysis (Suzuki and Brown, 1972). Molecular hybridization experiments using highly purified fibroin mRNA have established that there are one to three genes per haploid complement of DNA in all tissues investigated, including the posterior silk gland where fibroin is synthesized (Suzuki et al., 1972).
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Bombyx
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The crystalline structure of a kind of nature yellow Silk, derived from the parents of hybrid between Bombyx mori and a yellow cocoon silkworm(natural resources of color cocoon) , and a wildly silkworm silk fibroin were studied by Ramam spectrum, X-ray diffraction spectrum and FT-IR spectrum. Results shown that: All kinds of the silk fibroins were crystal high polymer composing with the major conformation of β-fold (SilkII). The crystallization degree of Bombyx mori silk fibroin and the nature yellow silk fibroin were higher than the wildly silkworm silk fibroin, and the crystalline structure of the nature yellow silk fibroin was more close to Bombyx mori silk fibroin.
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Fibroin
Antheraea pernyi
Random coil
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Antheraea pernyi silk fibroin has favorable biocompatibility, good bioactivity and controllable biodegradability, meeting the basic requirements of controlled drug release carriers. Enhancing the negative charge of silk fibroin could further increase the encapsulation and loading efficiency of positively charged drugs. In this study, Antheraea pernyi silk fibroin was chemically modified by methylglyoxal in aqueous solution. The electric charge properties of Antheraea pernyi silk fibroin were examined to characterize the modification, the results indicated that the isoelectric point of Antheraea pernyi silk fibroin decreased from 4.5 to 3.9, and the zeta potential reduced from-11.7 mV to-12.8 mV. Amino acid analysis and 1 H-NMR spectra showed that arginine residue of Antheraea pernyi silk fibroin side chain was modified by methylglyoxal for enhancing negative charge of silk fibroin. These results suggested that methylglyoxal-modified Antheraea pernyi silk fibroin could be considered as a potential starting material in loading positively charged drugs.
Antheraea pernyi
Fibroin
Biocompatibility
Sericin
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The extraction of fibroin (degumming) from the silk fibers impacts the properties of silk fibroin. However, the effect of the degumming method on structures and properties of regenerated Antheraea pernyi silk fibroin (ASF) biomaterials has not been investigated in detail. In this study, Na2CO3 and NaHCO3 are selected to remove sericin of Antheraea pernyi silks. The results show that the silk fibroin prepared by NaHCO3 degumming possessed a higher molecular weight, demonstrated by SDS-PAGE and viscosity testing. Na2CO3 degumming produced more low molecular weight silk fibroin protein below 40 kDa, whereas NaHCO3 processing produced more silk fibroin protein above 70 kDa. The increased molecular weight significantly improved the mechanical properties of ASF film. The enzyme degradation results indicated that the degradation rate of ASF film prepared by Na2CO3 degumming was faster in the early stage due to the presence of low molecular weight silk fibroin. After 21 days, the two silk fibroin films showed approximate degradation ratios. These results showed that the degumming conditions have a significant effect on the structure and properties of ASF biomaterials, and provide options for regulating the mechanical properties and degradation rate of ASF biomaterials.
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Antheraea pernyi
Sericin
Degradation
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Antheraea pernyi silk fibroin has a potential prospective in biomedical material field owing to its distinct advantages.A composite made of wild Antheraea pernyi silk fibroin and PVA was prepared in this study.To study the effects of process conditions on the conformation and mechanical properties of the composite,FTIR was applied and mechanical properties of the material were tested.Results show that PVA molecular weight,heating temperature,crosslinking,reinforcement and the concentration of denature solution all affect the conformation and properties of the composite.
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Fibroin
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Objective To study the growth of neurons on the regenerated silk fibroin films. Methods The attachment, morphology, growth and phenotype of subventricular zone-derived neurons cultured on the regenerated antheraea pernyi silk fibroin films and bombyx mori silk fibroin films were studied in vitro. Results Neurons cultured on the regenerated antheraea pernyi silk fibroin films showed a higher survival rate and complexity than those on bombyx mori silk fibroin films(P0.05). Conclusion The antheraea pernyi silk fibroin films are more suitable for the growth of neurons than bombyx mori silk fibroin films, while both have good biocompatibility.
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Antheraea pernyi
Sericin
Biocompatibility
Bombyx
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