Supplementary Figure 1 from Formate Supplementation Enhances Antitumor CD8<sup>+</sup> T-cell Fitness and Efficacy of PD-1 Blockade
Jared H. RoweIlaria EliaOsmaan ShahidEmily F. GaudianoNatalia E. SifnugelSheila JohnsonAmy G. ReynoldsMegan E. FungShakchhi JoshiMartin W. LaFleurJoon Seok ParkKristen E. PaukenJoshua D. RabinowitzGordon J. FreemanMarcia C. HaigisArlene H. Sharpe
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<p>Supplementary Figure 1 shows the approach for rapid magnetic bead based separation of OT-1 cells and peptide pulsed splenocytes for metabolomic analysis.</p>Keywords:
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Objective: To observe the level of IL 2 secreted by splenocytes of EL 4B7 1 + mixed culture immunized mice. Methods: CTLL 2 cells were used to detect the productivity of IL 2 secreted from splenocytes of EL 4B7 1 + cells mixed culture immunized mice. Results: The level of the production of IL 2 by splenocytes of EL 4B7 1 + mixed culture immunized mice was higher that of IL 2 produced by splenocytes of EL 4 immunized mice (P0 01) Conclusion: A high level of IL 2 can be secreted by splenocytes of EL 4B7 1 + cells mixed culture immunized mice.
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AIM To explore the substance basis of immunomodulating
components. METHODS Liuwei dihuang polysaccharides, CA43B and P3, were isolated from
liuwei dihuang decoction employing the method of close collaboration between chemical and
pharmacological studies under the guidance of activity evaluation. The action of
immunomodulation of CA43B and P3 on mouse splenocytes was evaluated with splenocyte
proliferation and antibody production response by TdR incorperation and plaqueforming cell
(PFC) assays, respectively, in vitro. RESULTS Both CA43B and P3 enhanced splenocyte
proliferation in normal mouse and senescenceaccelerated mouse (SAM) when used alone, but
did not promote splenocyte proliferation induced by Con A. The splenocyte proliferation was
inhibited when the doses of CA43B and P3 increased to 180 and 680 mmolL-1 respectively.
CA43B promoted the antibody production response induced by sheep red blood cells (SRBC)
also, and increased the number of PFC of the splenocytes. CONCLUSION CA43B and P3 are
probably active polysaccharides possessing immunomodulating effects.
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The growth of the syngeneic tumor MMT1 in C3H of mice was accompanied by significant changes in the spleen weight and in the number of nucleated cells in the spleen. Tumor excision led to the reduction of these indicators to the initial level. Adoptive transfer of splenocytes from tumor-bearing to intact mice exerted an inhibitory (on days 5, 14) or stimulatory (on day 25) action on the development of experimental metastases in intact animals depending on the tumor growth time. The result of splenocyte transfer from the operated on mice depended on the presence or absence in donors of tumor growth relapses (the development of lung metastases was inhibited only by splenocytes from donors with tumor relapses). Splenocyte transfer from mutant nude mice, both tumor-bearing and intact, produced an equipotent inhibitory effect. It is suggested that different mechanisms may be responsible for the development of metastasis inhibition in normal tumor-bearing mice and in tumor-bearers with T-cell system immunity deficiency.
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OBJECTIVE To assess the immunosuppressive effect of Artemisia vestita Wall Extract (AV-ext) on mice. METHODS The proliferative reaction of lymphocyte and the mixed lymphocytes reaction were used to determine the effects of AV-ext on the proliferation of mouse splenocyte in vitro and in vivo; Proliferative reaction of mouse splenocyte was used for detecting the effects of AV-ext on the level of IL-2 secreted by mouse activated splenocyte in vitro. Gelatin enzymogram method and adherence analytical method were employed to disclose the effects of AV-ext on mouse activated T-lymphocytes mobility and adherence. RESULTS 1-100 microg/mL AV-ext exerted no obvious toxicity to mouse splenocyte, but it had obvious inhibitory effect on proliferative reaction of mouse splenocyte and mixed lymphocytes reaction induced by ConA. It also had obvious inhibitory effect on the level of IL-2 secreted by mouse activated splenocyte, on the production of MMP-9 by mouse activated T-lymphocytes, and on adherence. 150 mg/kg and 300 mg/kg of AV-ext, given to mouse per os for 7 days, could inhibit the proliferation of splenocyte and the secretion of MMP-9 by activated splenocyte of mouse. CONCLUSION AV-ext can inhibit the cellular immune reaction of mouse obviously.
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A decrease of the natural killer activity (NKA) of C3HA splenocytes after transplantation of hepatoma 22a cells was connected to some extent with the suppressor action of macrophages and T-lymphocytes. Elimination of the macrophages from the splenocyte suspension led to the increase of NKA. Addition of the macrophages to the nonadherent cells resulted in the NKA inhibition to the level of nonfractionated splenocytes. T-cell elimination with anti-T-serum and a complement led to the increase of NKA as compared to NKA of the nonfractionated splenocytes.
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