Supplementary Figure 5 from slan<sup>+</sup> Monocytes Kill Cancer Cells Coated in Therapeutic Antibody by Trogoptosis
Giulia FinottiEnrica PietronigroCamillo BalanzinSilvia LonardiGabriela ConstantinMark P. ChaoCristina TecchioWilliam VermiMarco A. Cassatella
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<p>Control staining for CD19 and MDC8 on human tissue sections.</p>Objective Three different staining techniques were used to visualize S180 ascites smear morphology.The results were compared to find a best and simple staining technique showing most clear cytological characteristics.Method S180 ascites smear was stained by H.E.staining,Pap staining and fast staining,and the cell morphology was observed and compared under microscope.Results Among the 3 staining methods,the fast staining was most simple to operate and revealed cellular structures best.There was a best contrast among cytoplasm,nucleus and nucleolus in the smears stained by the fast staining,and the cell structures can be distinguished most clearly.Conclusion The fast staining is better than HE staining and Pap staining in S180 Ascites smear observation.
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Abstract This unit describes protocols for detecting proteins in SDS‐polyacrylamide gels. It describes methods for Coomassie blue and silver staining, as well as the fluorescent stains SYPRO Orange and Red. Staining with Coomassie blue is easier and more rapid; however, silver staining methods are considerably more sensitive and can thus be used to detect smaller amounts of protein. Alternative rapid staining procedures are provided for each method. Fluorescent staining is a popular alternative to the traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining. Curr. Protoc. Essential Lab. Tech . 6:7.4.1‐7.4.14. © 2012 by John Wiley & Sons, Inc.
Coomassie Brilliant Blue
Silver stain
Fluorescent staining
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Objective To evaluate a kind of modified reticulocyte(Ret) staining solution.Methods The observation test of the modified Ret staining solution was performed under the microscope,counted the staining time,analyzed the effective life and tested its accuracy and precision.Results The modified Ret staining samples had a lot of characters,such as not easily fade,few residues,clear visual field and bright background,etc.Compared with the routine staining solution-brilliant cresyl blue,the staining time was short(5 min) and the effective life was long(30 months).The accuracy of the modified staining solution was consistent with that of the brilliant cresyl blue(P 0.05),but the precision of the modified staining solution was better than that of the brilliant cresyl blue.Conclusion The modified Ret staining solution is an excellent staining.It is worthy to be spreaded in practice.
Cresyl violet
Positive staining
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Abstract172 liver samples from infants were analysed chemically for iron concentration, and histochemically for the presence of iron staining. A closer correlation was found between the amount of iron estimated chemically, and the presence of diffuse cytoplasmic staining than with the presence of granular staining. At levels of less than 5 mg/100 gm there was neither granular nor cytoplasmic staining present. Cytoplasmic staining was found in all sections from livers with an iron concentration of more than 30 mg/100 gm. Granular staining was not always seen above a level of 30 mg/100 gm, and some sections from livers with iron concentrations as high as 41 and 58 mg/100 gm, showed only cytoplasmic staining.
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Abstract This unit describes protocols for detecting proteins in SDS‐polyacrylamide gels. It describes methods for Coomassie blue and silver staining, as well as the fluorescent stains SYPRO orange and red. Staining with Coomassie blue is easier and more rapid; however, silver staining methods are considerably more sensitive and can thus be used to detect smaller amounts of protein. Alternative rapid staining procedures are provided for each method. Fluorescent staining is a popular alternative to the traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining.
Coomassie Brilliant Blue
Silver stain
Fluorescent staining
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Objective: To compare three kinds of staining methods for proteins in SDS PAGE. Methods: The proteins in SDS PAGE were stained with coomassie brilliant blue staining, copper staining and silver staining. Then the proteins were restained after coomassie brilliant blue staining or copper staining. Results:The silver staining was the most sensitive, the sensitivity of the copper staining was the former between that of silver staining and the coomassie brilliant blue staining. It took only five minutes to stain proteins with copper staining. After copper staining, the proteins could be restained by silver staining or coomassie brilliant blue staining. Conclusions: Copper staining could be the first choice for rapid analysis of proteins in SDS PAGE. [
Coomassie Brilliant Blue
Silver stain
Stain
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Objective:To compare Lyon′s PAS standardized staining and traditional PAS staining.Methods:The sections of the same tissue were stained with the above two methods simultaneously.The results and replicability of the two methods were observed.Results:The posititive products and replicability of Lyon′s PAS staining were stronger than that of traditional PAS staining in the same tissue-sections.Coclusion:Lyon′s PAS standardized staining method is better than the traditional PAS staining method.
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Objective: To compare two staining methods.Methods: Two staining methods were used to stain tissue sec-tion and cell smear respectively,the preparation methods and the staining effects of two staining methods were evaluated.Results: The modified staining buffer has less sediment,no oxidized membrane,don't need filtration,has shorter staining time and better staining effects.Conclusion:The modified staining method is better than the old method and can result in better staining effects.
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Protein-bands staining is an important step during the protein electrophoresis.Coomassie Brilliant Blue(CBB) staining,silver staining,fluorescent dyes staining and reverse staining were the main staining methods widely used.Combination CBB and Bismark Brown R can reduce staining/destaining times and enhance the effect of CBB.Modified silver staining using Gallyas INTENSIFYING can increase sensitivity of protein staining to visualize faint or invisible proteins separated on silver-stained SDS-PAGE.It is easier to distinguish spots from each other and the background on the silver-stained gels.Double staining of the CBB-stained gel with silver staining can also increase the sensitivity of detection of trace proteins.The recent new methods or modification of the existed methods were mainly followed sensitivity of the stain,simpleness of the procedure and the expense.How to reduce the silver staining background,replace the toxic reagents,avoid chemical modifications in the proteins during staining and improve the reverse-staining results may be the research direction in the future.
Silver stain
Coomassie Brilliant Blue
Stain
Negative stain
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A useful staining method for detection of cryptosporidian oocysts and sporozoites was developed and described. The modified Kohn's one-step staining technique with an additional modification, i, e., longer staining time and higher staining temperature than those originally described, was tested on fecal smears from cats infected with Cryptosporidium sp. By this improved staining procedure, the oocyst appeared as a slightly oval body containing four internal sporozoites colored blue to blue-gray. The oocyst wall was stained dark green to black. The morphological feature of the oocyst was recognized much more clearly by this staining technique than by such currently used techniques as acid-fast and Giemsa staining. This staining procedure proved to be simple and less costly and to secure a good preservation.
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