[Discovery, isolation and structural identification of alkaloid glycosides in six traditional Chinese medicine such as Coptis chinensis].
1
Citation
0
Reference
10
Related Paper
Citation Trend
Abstract:
Alkaloids are important active ingredients occurring in many traditional Chinese medicines, and alkaloid glycosides are one of their existence forms. The introduction of saccharide units improves the water solubility of alkaloid glycosides thus presenting better biological activity.Because of the low content in plants, alkaloid glycosides have been not comprehensively studied. In this study, ultrahigh performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry(UPLC-QTOF-MS/MS) was employed to identify and analyze the alkaloid glycosides in Coptis chinensis, Phellodendron chinense, Menispermum dauricum, Sinomenium acutum, Tinospora sagittata and Stephania tetrandra. The results showed that except Tinospora sagittata, the other five herbal medicines contained alkaloid glycosides. Furthermore, the alkaloid glycosides in each herbal medicine were identified based on UV absorption spectra, quasimolecular ion peaks in MS, fragment ions information in the MS/MS, and previous literature reports. A total of 42 alkaloid glycosides were identified. More alkaloid glycosides were identified in C. chinensis and Menispermum dauricum, and eleven in C. chinensis were potential new compounds. Furthermore, the alkaloid glycosides in the water extract of C. chinensis were coarsely se-parated by macroporous adsorption resin, purified by column chromatography with D151 cation exchange resin, ODS and MCI, combined with semi-preparative high performance liquid chromatography. Two new alkaloid glycosides were obtained, and their structures were identified by mass spectrometry and NMR data as(S)-7-hydroxy-1-(p-hydroxybenzyl)-2,2-N,N-dimethyl-1,2,3,4-tetrahydroisoquinoline-6-O-β-D-glucopyranoside and(S)-N-methyltetrahydropalmatubine-9-O-β-D-glucopyranoside, respectively. This study is of great significance for enriching the information about the chemical composition and the in-depth development of C. chinensis. Meanwhile, it can provide a reference for rapid identification and isolation of alkaloid glycosides from other Chinese herbal medicines.Keywords:
Coptis chinensis
Coptis
Objective To study the dynamic accumulation regulation of the effective components of Coptis chinensis,so to provide the experimental data of optimal harvest time of Coptis chinensis.Methods The samples of two to five years were gathered from the same glebe and time.Berberine and the total alkaloids were analyzed by HPLC and UV methods.Results The dynamic accumu- lation of effective components in the rootstalk of the Coptis chinensis had regularity in the certain extend.The contents of berberine in samples were almost the lowest in April in every year,which were mostly the highest in October and November from the samples grown 4～5 years,but the content of the component was mostly the highest in 5-year-old Coptis chinensis in the series samples. The content of the total alkaloids increased year by year in the rootstalk of the Coptis chinensis,which was mostly the highest in October and November in every year,but the content of the component was mostly the highest in 5-year-old Coptis chinensis in the samples.Conclusion According to the accumulation pattern of the effective components in the Coptis chinensis,the optimal harvest time is from September to November.
Coptis chinensis
Coptis
Cite
Citations (1)
Coptis chinensis
Coptisine
Coptis
Cite
Citations (41)
Objective:To determine the total alkaloids content of in Coptis chinensis.Method: Acidic dye colorimetry was used to determine the alkaloids in Coptis chinensis.Result: The concentration of berberine hydrochloride showed good linearity within the range of 4.176～16.68 μg/ml,r = 0.9997,the average recovery was 100.1%,RSD=1.84%,the total alkaloids from Coptis chinensis were determined as 0.112 g/g.Conclusion: The established method is simple,highly sensitive,accurate and reproducible,which can be used for determination of the total alkaloids in Coptis chinensis.
Coptis chinensis
Coptis
Coptisine
Colorimetry
Berberine hydrochloride
Harmine
Cite
Citations (2)
ObjectiveTo determine the total alkaloid and berberine contents in rhizome,fibre,lamina and petiole organs from Coptis omeiensis.MethodsThe total alkaloid and berberine contents were determined by UV and HPLC methods respectively.ResultsRhizome,fibre,lamina and petiole organs obtain alkaloid and berberine contents.ConclusionThe comparatively high contents in total alkaloid and berberine in the overground organs of Coptis omeiensis may provide guidance for tissue culture and resource protection.
Coptis
Coptis chinensis
Petiole (insect anatomy)
Coptisine
Cite
Citations (0)
Objective: To study the absorption and transport condition of the main components of different proportions of Fuzi(Aconitum carmichaeli debx.) with Huanglian(Coptis chinensis) in Caco-2 cells model. Methods: The Caco-2 cells model and high performance liquid chromatography(HPLC) method were establish to study the absorption and transport condition when the aconite-coptis ratio was 1 ∶1,1 ∶2,2 ∶1. The transmembrane transport amount and the apparent permeability coefficients were calculated the influence of the 3 ratios of the two drugs on absorption and transport. Results: When the aconite-coptis ratio was 1 ∶1,1 ∶2,2 ∶1,respectively,the transmembrane transport amounts(μg) at 150 min of berberine hydrochloride on AP→BL side are 0.625,0.161,0.055. On BL →AP side were 1.308,0.178,0.064,and the apparent permeability coefficients were 2.095,1.107,1.172,the transmembrane transport amounts(μg) at 150 min of hypaconitine on AP →BL side were 0.634,0.401,0.204,on BL →AP side were 0.534,0.108,0.067,and the apparent permeability coefficients were 0.842,0.268,0.328. Conclusion: The aconite-coptis ratio of 1∶1 was better than the ratio of 1∶2 and 2∶1. It is proved the best clinical ratio.
Coptis chinensis
Coptis
Aconitum
Aconitine
Cite
Citations (0)
To study the protective effect of Coptis and ginger Berberine on ethanol induced gastric lesion in rats,and discuss the mech anism.Methods: Observing damage of Coptis and ginger Berberine ethanol induced gastric lesion in rats evaluated by calculating lesion indices;detecting malondialdehyde(MDA) and superoxide dismutase(SOD) activity in plasma and gastric mucosal.Result:The group of Coptis and ginger Berberine gained less gastric mucosal damage and could inhibit the increase of MDA in plasma and gastric mucosa and prevented lower of SOD.Conclusion: Ginger Berberine have obvious protective effect on ethanol induced gastric lesion in ratscom pared with Coptis,and its mechanism may be related to inhibition of MDA increased and the recovery of SOD activity plasma and gas tric mucosal.
Coptis chinensis
Coptis
Malondialdehyde
Cite
Citations (0)
Objective To study the components and berberine of Coptis chinensis in extraction fluid by 75% ethanol,and research in vitro antimicrobial activity of Coptis chinensis and berberine hydrogchloride against H.pylori.Methods RP-HPLC column was used to study the components and berberine of Coptis chinensis in extraction fluid by 75% ethanol.The Kirby-Bauer was used to determine the sizes of inhibitory zones,and the multiple proportion dilution to determine the minimum inhibitory concentrations(MICs) of Coptis chinensis and berberine hydrogchloride.Results and Conclusion Coptis chinensis and berberine hydrogchloride have some in vitro antimicrobial activity against H.pylori.Coptis chinensis is better than berberine gydrogchloride in inhibiting H.pylori.
Coptis
Coptis chinensis
Cite
Citations (1)
ObjectiveThe soluble protein of Coptis deltoidea C.Y.Cheng et Hsiao.、Coptis 0meiensis.、 Coptis teeta Wall.and Coptis chinensis Franch.which come from different region were identified.MethodsProtein electrophoresis technology SDS-PAGE was applied.ResultsThe experiment shows that there were obvious differences between Coptis deltoidea C.Y.Cheng et Hsiao.and Coptis chinensis Franch.、little difference between Coptis omeiensis.and Coptis chinensis Franch.、a little difference between Coptis chinensis Franch.from different region.There was only one weak band in Coptis teeta Wall.ConclusionSDS-PAGE electrophoresis method can identify quality stability of Rhizoma Coptidis.
Coptis chinensis
Coptis
Cite
Citations (0)
Objective To explore the effect of ice treatment of subcutaneous hematoma after arteriopuncture with self-made Com-pound Liquid of Coptis Chinensis.Methods 60 patients with subcutaneous hematoma after arteriopuncture were randomly divided into control group and observation group equally.In control group,50% magnesium sulfate heat-cold therapy was applied,while in observation group,patients were treated with continuous ice treatment with self-made Compound Liquid of Coptis Chinensis.Hematoma size,pain and fever were observed and recorded.Results There was of statistical significance of the different effects of two groups.Conclusion Self-made Compound Liquid of Coptis Chinensis is effective in treatment of subcutaneous hematoma after arteriopuncture.
Coptis chinensis
Coptis
Cite
Citations (0)
Objective: To establish a method for determining the contents of four kinds of alkaloids in Coptis chinensis purified by macroporous absorption resin by Rp- HPLC. Methods: The macroporous absorption resin X- 5 was used to separate and purify the total alkaloids from Coptis chinensis. The HPLC method was used to determine the contents of epiberberine,coptisine,palmatine and berberine. Results: The average contents of epiberberine,coptisine,palmatine and berberine in Coptis chinensis with macroporous absorption resin were 1. 45%,2. 62%,7. 03%,17. 95% and the average contents without macroporous absorption resin were 0. 67%,1. 05%,1. 86% and 8. 54%. Conclusion: The alkaloids in Coptis chinensis can be effectively separated and purified with the macroporous absorption resin X- 5.
Coptisine
Coptis chinensis
Coptis
Jatrorrhizine
Cite
Citations (0)