Supplementary Figure 3 from A High-Content Screen Identifies Drugs That Restrict Tumor Cell Extravasation across the Endothelial Barrier
Georg HilfenhausAna MompeónJonathan FreshmanDivya P. PrajapatiGloria HernandezVanessa M. FreitasFeiyang MaAdam D. LangenbacherSnezana MirkovDana SongByoung-Kyu ChoYoung Ah GooMatteo PellegriniJau‐Nian ChenRobert DamoiseauxM. Luisa Iruela‐Arispe
0
Citation
0
Reference
10
Related Paper
Abstract:
<p>Effects of positive hits on endothelial barrier, cytoskeletal organization and tumor cell motility</p>Keywords:
Extravasation
Extravasation
Evans Blue
Leukocyte extravasation
Cite
Citations (11)
The plasma extravasation inducing activities of several chemical mediators (allergic agents: histamine, leukotriene C4 (LTC4) and platelet activating factor (PAF); neurogenic agents: substance P, capsaicin and carbachol) have been investigated and characterized in rat skin and trachea. Substance P, histamine, LTC4 and PAF induced dose-dependent plasma extravasation in rat skin. The activities of these mediators in inducing tracheal plasma extravasation were very different from those in the skin reactions. When these mediators were injected intravenously, substance P induced severe plasma extravasation, and the activities of histamine and PAF were weaker than that of substance P. When injected intratracheally, only substance P and capsaicin induced tracheal plasma extravasation, while none of the allergic mediators tested caused any plasma extravasation in the trachea. Carbachol did not induce any plasma extravasation in either skin or trachea. These results indicate that the stimulation of afferent substance P-containing nerve fibers has a more important role in the induction of tracheal plasma extravasation than that of allergic chemical mediators.
Extravasation
Capsaicin
Neurogenic inflammation
Platelet-activating factor
Leukotriene C4
Cite
Citations (0)
AbstractThe coordinated displacement of a cell is a highly complex process which relies on the integration of a series of signaling pathways and on the function of a large number of molecular components including all main structures of the cytoskeleton (1,2). Thus, a detailed knowledge of the regulation and function of the cytoskeleton is of central importance for the understanding of cell motility, and conversely, investigations of cell motility may shed light on the function of cytoskeletal components.KeywordsPersistence TimeNonlinear CurveCell SpeedChemotactic GradientChemotactic IndexThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Cell function
Cite
Citations (17)
Extravasation
Capsaicin
Evans Blue
Cite
Citations (6)
Recent studies suggest an active participation of postcapillary venules in inflammatory plasma extravasation. Intravital microscopic studies of rats' incisors show that Evans blue allows the demonstration of plasma extravasation in the dental pulp. This process takes place in the postcapillary venules, as can be seen from the staining of the interstice. Rather than depending on hydrodynamic pressure mechanisms, inflammatory plasma extravasation seems to be subjected to a direct regulation of vascular permeability.
Extravasation
Evans Blue
Vascular permeability
Leukocyte extravasation
Cite
Citations (0)
Pseudopodia
Cite
Citations (13)
Various dye fluids were injected into the blood vessels of frogs and rabbits and observationsin vivo were made of their patterns of extravasation.(1) A relation was noted between the site and mode of extravasation and the diffusibility of dyediffusibility itself being related to the size of dye particles.(2) Following intravenous injection the parts of blood vessels, excepting arterioles, where extravasation can occur were similar with both highly and poorly diffusible dyes. These parts extended from the arterioles to the venules. But the sequence of sites of extravasation and their frequency were in a reverse relationship. With highly diffusible dyes extravasation occurred in the sequence of precapillaries, capillaries, postcapillaries, venules and arterioles, and along the entire course of these vessels, so that the spread of extravasated dyes produced a color band form with the vessels along the long axis of the band. Contrary to this, with poorly diffusible dyes extravasation occurred first from the postcapillaires and venules, especially at the transitional portion of the two, then from the capillaries, and belatedly sometimes from the precapillaries. The mode of extravasation was sporadic and solitary, so that the spread of dye was oval in shape with the vessel running through the axis of the oval.(3) Following intra-arterial injection of highly diffusible dyes leakage of dye occurred in the sequence of arterioles, precapillaries, capillaries and postcapillaries, but not from the venules. With poorly diffusible dyes the sequence was capillaries, pre- and post-capillaries and venules. No leakage occurred from the arterioles. Thus, following intra-arterial injection of any type of dye, extravasation tended to occur in general from the arterial side, when compared with intravenous injection. However, the modes of spread were similar to those following intravenous injection, and highly diffusible dyes escaped from all over the vessels concerned forming a color band, and poorly diffusible dyes sporadically, from here and there, producing oval shaped forms.(4) There was no marked difference in the sites and modes of extravasation between frogs and rabbits.(5) Important factors determing the sites and modes of extravasation were believed to be the size of dye particles and the differences in structure of blood vessel walls according to locality (probably such as the grades in ease of disconnection of the junction between endothelial cells). However, when the local blood flow stops extravasation practically stops, so that the local blood pressure can also be said to promote extravasation. Various physical, chemical, physicochemical and biological factors that influence permeability are also believed to produce changes in size of particles and in structure of blood vessel walls.
Extravasation
Venule
Evans Blue
Cite
Citations (6)
Cell damage
Cell type
Cite
Citations (17)