Resveratrol promotes the differentiation of human umbilical cord mesenchymal stem cells into esophageal fibroblasts via AKT signaling pathway
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Abstract:
Resveratrol has been implicated in the differentiation and development of human umbilical cord mesenchymal stem cells. The differentiation of into esophageal fibroblasts is a promising strategy for esophageal tissue engineering. However, the pharmacological effect and underlying mechanism of resveratrol on human umbilical cord mesenchymal stem cells differentiation are unknown. Here, we investigated the effects and mechanism of resveratrol on the differentiation of human umbilical cord mesenchymal stem cells.Keywords:
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Objective Through to optimize the method about isolation and culture of human umbilical cord mesenchymal stem cells make us in-depth understanding of MSCs.Methods Optimize the way to get rid of the membrane and culture so as to achieve the optimization purpose.Result Achieve the purpose and do not affect the next experiment.
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Objective To explore the effects on umbilical cord blood mesenchymal stem cells (UCB-MSCs) combined with exogenous cytokines in supporting the expansion of umbilical cord blood mononuclear cells(UCB MNCs). Methods Mesenchymal stem cells (MSCs) were isolated from umbilical cord blood, and then were detected the cellular surface antigens. The UCB-MNCs were euhured in a serum-free system for 18 days. On day 0, 7, 10, 14 and 18, total mononuclear cells、CD34 cells、CD133 cells、cellular cycle and isolated and cultured from umbilical cord blood were negative for CD34 and CD133, but positive for CD29, The expansion combined with UCB-MSCs and exogenous cytokines had better effect than another groups. The above parameters reached the peak values on day 10. The culture system could maintain at least 18 efficiently support the expansion of UCB-MNCs.
Key words:
Umbilical cord blood; Cytokines; Mesenehymal stem cells; Cell culture
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Human umbilical cord mesenchymal cells isolated from the Wharton′s jelly of the umbilical cord,which possess potential of renewable,low risk of rejection and broad developmental.Human umbilical cord mesenchymal cells not only can be differentiated into islet-like cells under culture conditions of islet cell growth in vivo or in vitro,but also can secrete insulin to decrease blood glucose.Therefore,human mesenchymal stem cells in Wharton′s Jelly of the umbilical cord have the potential to become an excellent candidate in β-cell replacement therapy of diabetes.
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There are plenty of haematopoietic stem cells in umbilical cord blood, which are regarded as important resources for transplantation therapy. There are some arguments on whether or not mesenchymal stem cells(MSCs) exist in umbilical cord blood. Some researchers have such opinions that there exist a number of MSCs in umbilical cord blood as similar with one from bone marrow in many aspects. Others believe that the content of MSCs in umbilical cord blood is too low to expand in vitro. We summarized the data from last five years researches. On umbilical cord blood MSCs during last to help further research and application of this resource of MSCs.
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Obiective To culture and identify human umbilical cord blood MSCs(mesenchymal stem cells)using three phase approach.Methods Through three phases(removal ofred blood cell,briefly culturing and phase of lasting culturing)to separate MSCs from 4 human umbilical cord blood samples.Culture medium with low glucose DMEM was supplemented with 20% fetal calf serum.Isolated MSCs were passaged to the third generation for identifying.Results Three groups of MSCs were separated from 4 groups of samples.By flow cytometry analysising,cells expressed MSCs surface markers of CD29,CD44 and CD105 positively and CD34 negatively.The result of the differentiation of osteoblast-like cells derived from human umbilical cord blood was positive also.Conclusion We can easily and effectively separate MSCs from human umbilical Cord Blood by three phases approach.
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目的 探讨从人脐带血及脐带分离和培养间充质干细胞(MSCs)的方法,并分析MSCs的表面标记.方法 人脐带血按常规方法制备单个核细胞,利用MSCs贴壁生长的特性,经培养、换液、传代纯化MSCs;分离脐带华尔通胶(Wharton＇s jelly),采用组织块贴壁法获得脐带MSCs并传代.将传代的MSCs冻存,1个月后再复苏,观察复苏后MSCs的生长情况.利用FACScan流式细胞仪检测脐带血及脐带细...
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Mesenchymal stem cells (MSCs) are currently considered as 'medicinal signaling cells' and a promising resource in regard to cell-based regenerative therapy. Umbilical cord is a human term perinatal tissue which is easily attainable, and a promising source of stem cells with no associated ethical concerns. MSCs have been isolated from different regions of the umbilical cord and Wharton's jelly (WJ) is the gelatinous matrix that surrounds and provides protection to the umbilical cord blood vessels. Being more primitive, MSCs from human umbilical cord exhibit greater proliferative capacity and immunosuppressive ability as compared to adult stem cells which gives them a therapeutic advantage. To meet the requirements for cell therapy, it is important to generate MSCs at a clinical scale by following steps which are not time consuming or labor intensive. Here we present a simple, efficient protocol for isolation of MSCs from WJ of human umbilical cord by explant culture method which is reproducible and also, cost effective.
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Regenerative Medicine
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Cell therapy
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Mesenchymal stem cells isolated from umbilical cord blood capable of high proliferation potential and colony formation capacity in vitro and the ability to proliferate and differentiate to an osteogenic phenotype in culture.
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