Additional file 1 of On-farm colorimetric detection of Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in crude bovine nasal samples
Ana Pascual-GarrigosMurali Kannan MaruthamuthuAaron AultJosiah Levi DavidsonGrigorii RudakovDeepti PillaiJennifer KoziolJ. P. SchoonmakerTimothy A. JohnsonMohit S. Verma
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Additional file 1. Screened primer set sequences developed in [16].Keywords:
Pasteurella
A set of specific primers to amplify 457 bp DNA product was designed to detect swine Pasteurella multocida. The lung tissues or nasal swabs from pneumonic swine and atrophic rhinitis swine were streaked on TSA, then the bacteria clones were detected by the PCR and by the biochemical reaction after the bacteria were purified. With the mentioned-above methods, 66 strains of Pasteurella multocida were (isolated) from the different areas. The PCR result was quite coincident with that of the biochemical reaction. The detection results of 6 other swine respiratory pathogenic bacteria were negative by the set of primers. CFU 1×10~3/mL Pasteurella multocida template can be detected by the PCR.
Pasteurella
Pathogenic bacteria
Pasteurellosis
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Although Pasteurella multocida is a very common pathogen in animals, it has rarely been recognized as an infection of man. The first human infection was reported in 1913; between then and 1960 only 40 cases were reported in the world literature. The present patient became toxically ill following a bite from a domestic cat, and Pasteurella Multocida was grown on blood culture.The reason for the study is that the infecting agent can cause lesions in almost any body tissue, but up till now no report of liver involvement is available. Painful local inflammation after animal bites in humans deserves a search for Pasteurella multocida, which shows a sensitivity to penicillin unusual in a gram negative bacterium.
Bacteremia
Pasteurella
Pasteurellosis
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Білім берy қоғaмның экономикaлық дaмyының негізі, әлеyметтік тұрaқтылықтың фaкторлaрының бірі, хaлықтың рyхaни-aдaмгершілік әлеyетінің және интеллектyaлдық өсyінің қaйнaр көзі ретінде бaрлық yaқыттaрдa тaптырмaс құндылық болып есептеліп келеді. Aл қaзіргідей aдaм кaпитaлын қaлыптaстырy мен дaмытy мәселесін шешy негізгі міндет ретінде қaрaстырылaтын зaмaндa хaлықтың білімдік қaжеттіліктері өсіп, жоғaры, ортa aрнayлы, кәсіби қосымшa білім aлyғa үміткерлер сaны aртa түсyде. Бұғaн жayaп ретінде білім берy ұйымдaрының сaлaлaнyы aртып, әртүрлі типтегі оқy орындaрының сaны aртyдa, білім берyдің инфрaқұрылымы, бaсқaрy формaлaры, әдістемелік, ғылыми қызмет түрлері дaмyдa. Олaрды білім aлyшылaрдың жеке сұрaныстaры мен мүмкіндіктеріне бaғыттay күшейтілyде. Осығaн орaй білімнің сaпaсынa қойылaтын тaлaптaр aртып, бұл сaлaның әлеyметпен өзaрa әрекеттестігіне негізделген құрылымдық – қызметтік дaмyының көкейтестілігі aртyдa. Мaқaлaдa «серіктестік», «әлеyметтік серіктестік», «білімдегі әлеyметтік серіктестік» ұғым- дaрының мәні aшылып, олaрдың қaлыптaсy және дaмy үрдісіне шолy жaсaлaды, жоғaры оқy орындaрындa педaгогтaрды дaярлayдa әлеyметтік серіктестердің әлеyетін пaйдaлaнyдa бaсшылыққa aлынaтын ұстaнымдaр мен тиімді жолдaры сипaттaлaды. Түйін сөздер: серіктестік, әлеyметтік серіктестік, білімдегі әлеyметтік серіктестік, бірлескен әрекет ұстaнымдaры, әлеуметтік серіктестік әлеуеті. Обрaзовaние является основой экономического рaзвития обществa, одним из фaкторов социaль- ной стaбильности, источником дyховно-нрaвственного потенциaлa и интеллектyaльного ростa людей и во все временa считaлось незaменимой ценностью. И в нaстоящее время, когдa решение проблемы формировaния и рaзвития человеческого кaпитaлa рaссмaтривaется кaк основнaя зaдaчa, рaстyт обрaзовaтельные потребности людей, yвеличивaется количество желaющих полyчить высшее, среднее, специaльное, профессионaльное дополнительное обрaзовaние. В ответ нa это yсиливaется рaзветвленность обрaзовaтельных оргaнизaций, yвеличивaется количество обрaзовaтельных оргaни- зaций рaзличного типa, рaзвивaются инфрaстрyктyрa обрaзовaния, формы yпрaвления, методическaя и нayчнaя деятельность. Yсиливaется их ориентaция нa индивидyaльные потребности и возможности обyчaющихся. В связи с этим повышaются требовaния к кaчествy обрaзовaния, возрaстaет знaчение стрyктyрно-фyнкционaльного рaзвития этой сферы нa основе взaимодействия с обществом. В стaтье рaскрывaется знaчение понятий «пaртнерство», «социaльное пaртнерство», «социaльное пaртнерство в обрaзовaнии», рaссмaтривaется процесс их стaновления и рaзвития, описывaются рyко- водящие принципы и эффективные способы использовaния потенциaлa социaльных пaртнеров в подготовке педaгогических кaдров в высших yчебных зaведениях. Ключевые словa: партнерство, социaльное пaртнерство, социaльное пaртнерство в обрaзовaнии, принципы совместного действия, поненциал социального партнерство. Education is the basis of the economic development of society, one of the factors of social stability, a source of spiritual and moral potential and intellectual growth of people and has always been considered an irreplaceable value. And at the present time, when the solution of the problem of the formation and development of human capital is considered as the main task, the educational needs of people are growing, the number of people wishing to receive higher, secondary, special, professional additional education is increasing. In response to this, the branching of educational organizations is increasing, the number of educational organizations of various types is increasing, the infrastructure of education, forms of management, methodological and scientific activities are developing. Their focus on the individual needs and capabilities of students is increasing. In this regard, the requirements for the quality of education are increasing, the importance of the structural and functional development of this sphere on the basis of interaction with society is increasing. The article reveals the meaning of the concepts of "partnership", "social partnership", "social partnership in education", examines the process of their formation and development, describes the guidelines and effective ways to use the potential of social partners in the training of teachers in higher educational institutions. Keywords: partnership, social partnership, social partnership in education, principles of joint action, the potential of social partnership.
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Haemophilus somnus, Pasteurella haemolytica, Pasteurella multocida, and Actinobacillus pleuropneumoniae from cattle and pigs with respiratory disease were used to evaluate the RapID NH system (Innovative Diagnostics, Atlanta, Ga.). Minor modifications of the RapID NH system to include animal source and growth requirements would permit the identification of all isolates tested.
Actinobacillus pleuropneumoniae
Pasteurella
Actinobacillus
Pasteurellosis
Pleuropneumonia
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Altogether 309 samples from 187 pigs apparently healthy, 77 slaughtered for pork, 25 dead and 22 ailing pigs with gross lesions and clinical symptoms suggestive of pasteurellosis were screened for isolation of Pasteurella. Fifty (26.73%) animals were positive for Pasteurella species, form dead pigs (52%). Strains (63%, 20.38%) of Pasteurella were isolatted consisting 52 (82.54%) Pasteurella multocida and 11 (17.46%) Manneimia (Pasteurella) haemolytica. Recovery of Pasteurella multocida was the highest form lung followed by nasal swabs while M.(Pasteurclla) haemolytica could be recovered only from lung followed by nasal swabs. Among the 52 Pasteurella multocida isolated isolates 26 (50%) belonged to serotype A, 10 (19.23%) to serotype B while 16 (30.76%) were untypable. All the 11 isnlales of M.(Pasteurella} haemolytiea strains were untypable. Strains of Pasteurella mliltocida were highly sensitivity to cephalexin (100%) followed by nortloxacin (96.15%) and gentamicin (88.46%) and were least sensitive to lincomycin (7.69%). Similar type of sensitivity towards cephalexin and nortloxacin (100%) was also observed among the strains of M. (Pasteurella) haemolytica.
Pasteurella
Pasteurellosis
Isolation
Lincomycin
Antibiogram
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Deoxyribonucleic acid (DNA)-DNA hybridization was used to determine the genetic relationships among a variety of previously established or proposed species of Pasteurella and their positions within the family Pasteurellaceae Pohl 1981. Our results indicated that the genus Pasteurella sensu stricto, which can be separated from the Actinobacillus group, consists of at least the following 11 species: Pasteurella multocida, with three subspecies (P. multocida subsp. multocida, P. multocida subsp. septica, and P. multocida subsp. gallicida); Pasteurella dagmatis sp. nov., containing organisms previously labeled Pasteurella "gas," Pasteurella new species 1, or Pasteurella pneumotropica type Henriksen; Pasteurella gallinarum; Pasteurella canis sp. nov., previously labeled P. multocida biotype 6 or "dog type" strains; Pasteurella stomatis sp. nov., which contains Pasteurella strains isolated from dogs and cats; Pasteurella avium (Hinz and Kunjara) Mutters et al. 1985; Pasteurella volantium Mutters et al. 1985, a new species consisting of V-factor-requiring strains that occur in humans and birds; Pasteurella anatis sp. nov.; Pasteurella langaa sp. nov., containing strains previously designated taxa 1 and 4 of Bisgaard; and two new species, which were provisionally designated Pasteurella species A and Pasteurella species B. The previously recognized taxa Pasteurella ureae, Pasteurella haemolytica biotypes A and T, Pasteurella testudinis, and P. pneumotropica biotypes Jawetz and Heyl do not belong to the genus Pasteurella but are more closely related to the Actinobacillus group. The exact taxonomic positions of Pasteurella aerogenes, P. multocida biotype 1, group HB-5, "Pasteurella piscicida," the SP group, and the bovine lymphangitis group are still unknown, but these organisms do not belong to the genus Pasteurella.
Pasteurella
Pasteurellosis
Actinobacillus
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Abstract : Investigations on the R-infection of strains of various Pasteurella species made it possible to establish the R-infection of all species tested. One difference between the Pasteurella species, such as P. pseudotuberculosis, P. pestis, and Pasteurella 'X', respectively, the other Pasteurella species which are not related to them, such as P. multocida, P. haemolytica, P. pneumotropica, and P. anatipestifer -- which might be categorized among the family of the Enterobacteriaceae, consisted in the fact that the two first-named species and Pasteurella 'X' accepted the R-factors with a higher frequency than the other Pasteurella species. R-infected strains of all Pasteurella species transferred the R-factor to an acceptor (recipient) strain.
Pasteurella
Pasteurellosis
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This study was described for the nature of the pathpgenesis of bacteria Pasteurella multocida which was isolated from infected man made comparison between these bacteria and those from infected farm animals. The percentage of Pasteurella multcida diagnosed bacteria from animals and human was 29.4% and 16.9% respectively. Comparing to other culture media Pasteurella multocida selective agar medium was characterized by its selectivity and sensitivity and then was attempt for biotyping species and subspecies of isolated Pasteurella from animals and human samples were successfully achieved. Pathogenicity test was performed on mice, only nine human isolatetes and twenty-one animal isolates from Pasteurella multocida were virulent. Todistinguish between the pathogenesis of human and animal isolates, one isolated from human and animal were chosed, in addition to the standared strain. The mice had been experimentally infected by three different ways, I/P, I/T, I/Eye. The results were showed that Pasteurella multocida can produce lesions as fibrinous suppurative pneumonia in lungs, liver and spleen which were detected histopatho logically. However the animal isolates were more virulent than human or standared strain.
Pasteurella
Pasteurellosis
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A modified barrier system for maintaining Pasteurella multocida-free rabbits in an animal facility also housing Pasteurella multocida-infected rabbits was implemented. All research and laboratory animal technical personnel performed their duties involving non-infected rabbits prior to working with infected rabbits housed in separate rooms of the facility. Bacterial culture data involving mature rabbits indicated a 17% infection rate when Pasteurella-free rabbits were housed in a conventional manner for 3-6 months prior to institution of the modified barrier. No transmission of Pasteurella multocida from infected to non-infected rabbits housed in separate rooms was observed during 6 months of maintenance after initiation of the modified barrier system.
Pasteurella
Pasteurellosis
Rabbit (cipher)
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A cross-sectional study was conducted from October, to 2013 to April 2014 with the aim of isolating and identifying pasteurella species in lung lesion (pneumonic lung) of apparently healthy goat slaughtered at Hasin Nuru Export Abattoir. All goats were originated from Bale and Harar area. Samples were collected aseptically from the lung lesion of 384 investigated Goat and standard microbiological techniques were used for isolation and identification of pasteurella species. From the 384 specimen collected for bacteriological examination, 301 were positive for pasteurella species . A total 301 pasteurella species recovered in which 274 and 27 of them were Manehemia hemolytica and Pasteurella multocida , respectively. The isolation rates of Manehemia hemolytica was 74.4% and Pasteurella multocida was 7%. Therefore, the major pasteurella species isolated from lung lesion of caprine in this study was Manehemia hemolytica and, Pasteutella multocida was the minor isolates. In conclusion pasteurella organism especially Manemia hemolytica is one of the most cause of pneumonic pasteurellosis in caprine and rarely Pasteurela multocida so Chemoprophylaxis need to be given to small ruminants prior to transportation or other stresses.
Pasteurella
Pasteurellosis
Isolation
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