Additional file 6 of Integrating GWAS, linkage mapping and gene expression analyses reveals the genetic control of growth period traits in rapeseed (Brassica napus L.)
Tengyue WangLijuan WeiJia WangLing XieYang Yang LiShuyao RanLanyang RenKun LuJiana LiMichael P. TimkoLiezhao Liu
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Additional file 6: Table S4. Genes identified in LD block or 300 kb up- and down-stream of significant SNPs identified in GWAS.Keywords:
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Abstract The association of sinapine with the fishy or crabby odour present in eggs laid by certain brown egg laying hens when fed diets containing rapeseed meal indicates the desirability of removing sinapine from the original rapeseed. A method is presented whereby seeds and cotyledons of Brassica napus and B. campestris may be selected for low levels of sinapine. The method, based on microextraction, rapid chromatographic separation of sinapine and visual estimation of its fluorescence allows detection of 0.025 μg sinapine and enables 300‐400 seed samples to be screened daily.
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Two proteins, previously described by the authors as BI (S 0 20 , buffer 12 S) and AIVS (S 0 20 , w 1.7 S) have been isolated from eight varieties of rapeseed belonging to Brassica campestris L. and Brassica napus L. species. These proteins have similar chromatographic and electrophoretic characteristics but differ in amino acid composition, particularly with regard to the sulfur-containing amino acids. One of the rapeseed samples was obtained from plants grown on sulfur-deficient soil. The sulfur deficiency produced, in the mature seed, a much reduced protein content and appeared to affect the structure of the protein BI.
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An experiment with M1 and M2 generation following treatment of one variety each of mustard (Brassica juncea) and rapeseed (Brassica campestris var. toria) with three different doses of Ethyl methane sulphonate (EMS) and Diethyl sulphate (DES) revealed that the mustard variety is less sensitive than rapeseed variety for EMS whereas the reverse trend was noticed for DES in respect of mutagen induced stress response. The EMS and DES mutagenesis may be recommended for improvement of the rapeseed and mustard varieties respectively. The analysis of stress response is considered helpful in predicting changes in mean and variability of some traits like siliqua per plant and plant height in favourable direction
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The sulfur status of rapeseed (Brassica napus and Brassica campestris) plants grown in field studies was assessed using a variety of plant indices; percent sulfur, percent hydriodic acid reducible sulfur (HI-S in plant dry matter), HI-S:total S ratio, and total N:total S ratios. Of these, HI-S:total S determined at the rosette growth stage was the most accurate and consistent index of seed yield. A growth chamber study indicated that the HI-S:total S ratio would not be affected by nitrogen fertilizer application at normal field rates. Key words: Sulfur, deficiency, rapeseed (Brassica spp.), plant analysis
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