Additional file 4 of Whole genome sequencing of Moraxella bovis strains from North America reveals two genotypes with different genetic determinants
Emily L. WynnMatthew M. HilleJohn Dustin LoyGennie SchullerKristen L. KuhnAaron M. DickeyJames L. BonoMichael L. Clawson
0
Citation
0
Reference
10
Related Paper
Abstract:
Additional file 4.Keywords:
Moraxella (Branhamella) catarrhalis
From 1953 to 1980 the Centers for Disease Control received 933 isolates of bacteria belonging to species of the genus Moraxella, Moraxella-like Moraxella urethralis, now renamed Oligella urethralis, unnamed groups M-5 and M-6, and Kingella kingae. Ordinarily sterile sites were the source of 233 isolates. Moraxella nonliquefaciens, the most common isolate (356 strains), was recovered from upper respiratory or ocular sites in 208 (58%) of the cases. Moraxella osloensis was next most common (199 strains) but was the most frequent blood isolate (44 cases). K. kingae appeared especially invasive, with 58 of 78 isolates from blood, bone, or joint. Of the K. kingae strains, 75% were recovered from children under 6 years, compared with 23% of the other strains from that age group (P less than .01). Of the 74 isolates of group M-5, 53 were from wounds caused by dog bites; no other organism in this series was recovered from such wounds. Sixteen of the 28 M. urethralis isolates were from urine. Cases occurred as single infections, with no evidence of clusters. Of patients with infection of ordinarily sterile sites, 9.3% died; only bacteremia, meningitis, and empyema caused fatalities. Most referring laboratories (98%) had not identified the organisms to species, and only 30% had identified them to correct genus. Susceptibility testing by broth dilution revealed low MICs of penicillin (mean, 0.3; 64% less than 1 micrograms/mL). Moraxella, M. urethralis, M-5, M-6, and Kingella are important but frequently misidentified pathogens for humans; penicillin appears to be the treatment of choice.
Moraxella (Branhamella) catarrhalis
Kingella kingae
Neisseriaceae
Bacteremia
Cite
Citations (88)
The article presents the results of comparative studies in infectious and invasive keratoconjunctivitis of cattle for 2023. 542 conjunctival samples of cattle from economic entities of Akmola, Zhetysu, Turkestan and Kostanay regions were studied. Real-time polymerase chain reaction was carried out with a set of «PCR-Moraxella-RV» reagents to identify three causative agents of Pink eye - Moraxella bovis, Moraxella ovis, Moraxella bovoculi. 15 positive samples from the Akmola region were identified as Moraxella bovoculli (100%), 33.3% were identified as Moraxella ovis,and 6.7% were identified as Moraxella bovis. During a microscopic examination of conjunctival swabs from the same animals, thelazias were dedected in 224 samples, which amounted to 43.1%. В статье приводятся результаты сравнительных исследований при инфекционном и инвазионном кератоконъюнктивитах крупного рогатого скота за 2023 год. Было исследовано 542 конъюнктивальных смывов крупного рогатого скота хозяйствующих субъектов Акмолинской, Жетысуской, Туркестанской и Костанайской областей. Полимеразно-цепная реакция в режиме реального времени проводилась набором реагентов «ПЦР-Моракселла-РВ» для выявления трех возбудителей моракселлеза - Moraxella bovis, Moraxella ovis, Moraxella bovoculi. В 15 положительных пробах из Акмолинской области, идентифицированных как Moraxella bovoculli (100%), 33,3% опознаны как Moraxella ovis, а 6,7% – Moraxella bovis. При микроскопическом исследовании конъюнктивальных смывов от этих же животных, телязии были обнаружены в 224 пробах, что составило 41,3%.
Moraxella (Branhamella) catarrhalis
Cite
Citations (0)
The biochemical characteristics of 59 strains of Moraxella urethralis from clinical specimens, primarily from urine and the female genital tract, were studied. The characteristics included (i) the inability to acidify carbohydrate substrates, (ii) the ability to produce phenylalanine deaminase, (iii) the ability to reduce nitrite, (iv) the lack of urease activity, and (v) the ability of most strains to alkalinize citrate. A means of differentiating M. urethralis from Moraxella osloensis and Moraxella phenylpyruvica was determined.
Moraxella (Branhamella) catarrhalis
Cite
Citations (13)
Moraxella (Branhamella) catarrhalis
Isolation
Biovar
Cite
Citations (2)
В статье приведены данные о разработке синтетических олигонуклеотидов для выявления одного из возбудителей моракселлеза крупного рогатого скота Moraxella ovis. Дезоксирибонуклеиновая кислота референтного штамма Moraxella ovis ATCC-33078 был выделен набором реагентов «РИБО-сорб» и PureLink Genomic DNA Mini Kit. Аналитическая чувствительность с использованием набора олигонуклеотидов в составила 10-5, что соответствует 21 копий или 50 фг в реакции. Аналитическая чувствительность синтетических праймеров проверялась десятикратным разведением дезоксирибонуклеиновой кислоты Moraxella ovis. Специфичность набора олигонуклеотидов Moraxellа доказана тестированием близкородственных микроорганизмов, т.е. референтными штаммами Moraxella bovis 17948ТМ және Moraxella bovoculi 1259ТМ и возбудителей некоторых зоонозов. Использование набора олигонуклеотидов в полимеразноцепной реакции в режиме реального времени показало 100% специфичность для определения культур Moraxell. Набор олигонуклеотидов Moraxella ovis позволяет разработать экспресс-тест-систему с использованием полимеразной цепной реакции в режиме реального времени для диагностики возбудителя кератоконъюнктивита. В дальнейшем полученный научный результат позволит разработать мультиплексную полимеразную цепную реакцию в режиме реального времени, позволяющую одновременно выявлять возбудителей Moraxella bovis, Moraxella bovoculi и Moraxella ovis.
Moraxella (Branhamella) catarrhalis
Ovis
Cite
Citations (0)
1
Taxonomic Review: Family Moraxellaceae
2
Acinetobacter
3
Moraxella
4
Moraxella nonliquefaciens
5
Moraxella lacunata
6
Moraxella osloensis
7
Minor Species
8
Animal-Associated Species
9
Moraxella (Branhamella) Catarrhalis
10
Acknowledgments
Keywords:
Acinetobacter and Moraxella;
genus Moraxella-several taxonomic revisions;
Acinetobacters-saprophytic and ubiquitous;
virulence and pathogenic factors;
management, therapeutic options and susceptibility testing;
Moraxella nonliquefaciens-commonly isolated species of genus;
M. catarrhalis-gram-negative diplococci;
M. catarrhalis-susceptible to penicillin and β-lactamase negative
Moraxella (Branhamella) catarrhalis
Neisseriaceae
Cite
Citations (3)
Moraxella sp. CK-1이 Anabaena cylindrica 세포를 분해하는데 있어 숙주의 개체군 크기는 1∼5X10^6cells/ml의 범위에서 세균의 분해활성에 큰 차이를 보이지 않으며, 세균의 개체수가 서로 다를 경우에는 숙주와 세균의 세포수가 각각 5x10^6cells/ml정도로 그 비율이 1:1에 가까울 때 가장 높은 활성을 나타내었다. Moraxella sp. CK-1의 숙주세포 분해효율은 숙주세포가 왕성히 생장할 때 가장 높으며, 광합성 억제제인 DCMU를 처리하거나 빛을 차단할 경우에도 세균의 분해활성에는 큰 변화가 없었다. 에너지원인 glucose는 분해세균의 활성을 억제하였으며, 질소원인 질산염은 5∼10mM농도에서 분해활성을 촉진시키는 작용을 하였다. 분해세균의 숙주세포 분해효율은 pH 6.0∼9.0 사이의 수소이온농도에서 유사한 반면 pH10.0에서는 분해작용이 거의 나타나지 않았다. A. cylindrica와 Moraxella sp. CK-1의 혼합배양액을 200rpm의 속도로 진탕배양할 경우에도 세균의 숙주 분해가 이루어진다. 이상의 결과로부터 Moraxella sp. CK-1은 A. cylindrica의 생장, 특히 질소고정능에 의존적으로 생장하면서 숙주세포를 분해하는 물질을 분비한다는 사실을 확인하였다.
Moraxella (Branhamella) catarrhalis
Cite
Citations (0)
Abstract The Judicial Commission of the International Committee for Systematics of Prokaryotes rules that the following names should have been included on the Approved Lists of Bacterial Names, Moraxella (subgen. Branhamella Bøvre 1979), Moraxella (subgen. Moraxella Lwoff 1939), Moraxella (subgen. Branhamella Bøvre 1979) catarrhalis, Moraxella (subgen. Branhamella Bøvre 1979) caviae, Moraxella (subgen. Branhamella Bøvre 1979) ovis, Moraxella (subgen. Moraxella Lwoff 1939) atlantae, Moraxella (subgen. Moraxella Lwoff 1939) bovis, Moraxella (subgen. Moraxella Lwoff 1939) lacunata, Moraxella (subgen. Moraxella Lwoff 1939) nonliquefaciens, Moraxella (subgen. Moraxella Lwoff 1939) osloensis, Moraxella (subgen. Moraxella Lwoff 1939) phenylpyruvica. Proposals to alter Rule 34a were rejected.
Moraxella (Branhamella) catarrhalis
Cite
Citations (22)
Moraxella (Branhamella) catarrhalis
Cite
Citations (16)