Femtomolar detection of staphylococcal enterotoxin ‘B’ using a fluorescent quantum dot based hybrid Apta-immunosensor
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Objective The study was made to investigate and analyze the status of the contamination of staphylococcus aureus and enterotoxin in bioproducts of shrimp.Methods Staphylococcus aureus was tested according to GB/T4789.10-2003 and its enterotoxin was determined by mini-VIDAS Automatic Luciferaseimmunization Analyzer.Results It is found out that detection rate of staphylococcus aureus is 68.2%for quick freezing shrimps,with detection rate of 40.0%for enterotoxin Staphylococcus aureus thereof.Detection rate of staphylococcus aureus is 31.8%for dehydration shrimps,with detection rate of 100.0%for enterotoxin Staphylococcus aureus thereof.Conclusion It revealed that contamination of staphylococcus aureus and enterotoxin in the shrimp byproducts is serious and enterotoxin detection of Staphylococcus aureus in the shrimps byproducts should be strengthen.
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We examined changes in the extent of fluorescence resonance energy transfer (FRET) between two different fluorochromes attached to a single oligonucleotide in the presence or absence of target nucleic acids with a specific sequence and a higher-ordered structure. In our system, FRET was maximal when probes were free in solution and a decrease in FRET was evidence of successful hybridization. Incubation of the probe with a single-stranded complementary oligonucleotide reduced the FRET. While, a small change in FRET was also observed when the probe was incubated with an oligonucleotide in which the target site had been embedded in a stable hairpin structure. These results indicate that this spectrofluorometric method and FRET probes can be used to estimate the efficacy of hybridization between a probe and its target site within highly ordered structures. It should help us to estimate the suitability of designed functional molecules, such as antisense DNA and RNA and ribozymes, that target to specific sites.
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This chapter contains sections titled: A Rosy Crystal Ball View of FRET Do Not Ask What FRET Can Do for You, Ask What You Can Do for FRET FRET: Future Research with an Exciting Technology Future of FRET Outlook on Single–Molecule FRET Outlook on FRET with fluorescent proteins Luminescent Nanoparticles: Scaffolds for Assembling "Smarter" FRET Probes
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Objective:To compare some pathogenic substances of Staphylococcus aureus(S.aureus) with enterotoxin B and that of its L form.Methods:Coagulase,heat stable nuclease and enterotoxin B(SEB) generated from S.aureus L form were detected and compared with that from S.aureus .Results:In comparing with original S.aureus ,the L form showed delaying plasma coagulation,smaller in clear ring of heat stable nuclease,but no significantly decrease in optic density(OD) of SEB.Conclusions: S.aureus L form still generated some pathogenic substances,but getting less.
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The diagnostic application of an enzyme immunoassay for Clostridium perfringens type A enterotoxin was evaluated. Test results from 100 individuals associated with C. perfringens gastroenteritis outbreaks and 111 control individuals were included. The assay sensitivity was 93.7%, and the assay specificity was 98.7%.
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An enzyme immunoassay (EIA) system for detection of staphylococcal enterotoxin, type C, has been developed. The sensitivity of the system is 1 ng/ml. The optimum EIA parameters have been worked out. The absence of false positive results with heterologous toxins confirms the specificity of the assay system. The possibility of the detection of staphylococcal enterotoxin, type C, in staphylococci isolated from different sources has been shown.
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