Characterization of Anthocyanins and Their Antioxidant Activities in Indian Rose Varieties (Rosa × hybrida) Using HPLC
Poonam KumariDhandapani RajuK.V. PrasadSupradip SahaSapna PanwarSurinder PaulNamita BanyalAarti BainsPrince ChawlaMelinda FogarasiSzabolcs Fogarasi
23
Citation
39
Reference
10
Related Paper
Citation Trend
Abstract:
The present study was designed to explore the anthocyanin profile and antioxidant activities in Indian rose varieties (Rosa × hybrida). Among fifty varieties, Ashwini recorded the highest total phenolic content (427.59 ± 3.47 mg GAE/100 g) along with the highest FRAP (397.15 ± 0.82 µmol trolox/g) and DPPH free radical scavenging activity (93.47 ± 0.19%) on a fresh weight basis. A significant positive correlation was observed between total anthocyanin content, total phenolic content, and antioxidant activities. Four distinct clusters were formed according to total anthocyanins, total phenols, and antioxidant activities; white- and yellow-colored varieties were most distant from red ones. Principal component analysis revealed that variable total anthocyanin content contributed to the maximum variation among the fifty rose varieties studied. Highly anthocyanin-rich rose varieties were characterized by high-performance liquid chromatography coupled with a photodiode array detector (HPLC-PAD), which identified two major components of anthocyanins, i.e., cyanidin 3,5-di-O-glucoside and pelargonidin 3,5-di-O-glucoside. Cyanidin 3,5-di-O-glucoside was the predominant anthocyanin in red- and pink-colored varieties, whereas pelargonidin 3,5-di-O-glucoside was the major one in the orange variety. The maximum cyanidin 3,5-di-O-glucoside content was recorded in variety Ashwini (497.79 mg/100 g), whereas the maximum pelargonidin 3,5-di-O-glucoside content was recorded in Suryakiran (185.43 mg/100 g). It is suggested that the rose varieties with high anthocyanin content and antioxidant activity can be exploited as a potential source of nutraceuticals in the food industry.Keywords:
Pelargonidin
Anthocyanidins
Anthocyanidins
Pelargonidin
Anthocyanidins
Cite
Citations (25)
ABSTRACT The distribution of individual anthocyanin pigments in the fruits of 43 Rubus clones was determined by TLC and densitometry. Quantitative estimates of anthocyanin pigments were based on absorbance at 533 nm. The fruit of all red and black raspberries and blackberries contained cyanidin 3‐glucoside. Some blackberries also contained cyanidin 3‐rutinoside. Cyanidin 3‐rutinoside and cyanidin 3‐glucosylrutinoside were also present in most red raspberries. Cyanidin 3‐sophoroside was present only in red raspberry and red raspberry hybrids while cyandin 3‐sambubioside and cyanidin 3‐xylosylrutinoside were found only in black raspberries. R. crataegifolius, R. morifolius, R. illecebrosus and R. parviflorus each contained pelargonidin glycosides as major pigments. Black raspberry had the highest anthocyanin content, over 200 mg/100g of fruit, and red raspberry had the lowest, less than 60 mg/100g. Blackberry was generally intermediate in anthocyanin content between red and black raspberries.
Blowing a raspberry
Rubus
Pelargonidin
Anthocyanidins
Cite
Citations (120)
Peonidin
Pelargonidin
Anthocyanidins
Anthocyanidins
Petunidin
Petal
Cite
Citations (1)
Solanum tuberosum
Anthocyanidins
Cite
Citations (33)
Pelargonidin
Anthocyanidins
Epistasis
Strain (injury)
Cite
Citations (35)
Abstract Anthocyanins are economically valuable phytochemicals of significant relevance to human health. Multiple fruit and vegetable sources for industrial-scale anthocyanin purification exist, however, each source has distinct anthocyanin levels and profiles conferred by modifications to the central anthocyanidin core. In this study, we assessed three purple-fleshed and one orange-fleshed cultivars of sweet potato, with the goal of studying their anthocyanin yield and diversity when this warm-weather crop is grown in cooler, northern latitudes. Comparison of multiple anthocyanin extraction methods revealed acidified ethanol extraction of lyophilized roots as the optimal method, producing a high, average yield of ∼800 mg anthocyanins/100g dry weight. Mass spectrometric analysis of sweet potato extracts identified eighteen high-confidence anthocyanins – all derived from peonidin and cyanidin cores – contributing to over 90% of the total anthocyanin signal. The concentrations of different anthocyanins were variable between the three purple-fleshed cultivars, while low anthocyanin accumulation was observed in the orange-fleshed cultivar. Further assessment of the untargeted high-resolution mass spectrometry data using MS/MS molecular networking revealed existence of low-abundance anthocyanins with delphinidin and pelargonidin cores, as well as over 250 peaks comprising of potential anthocyanins and flavonoids. These results provide a comprehensive insight into anthocyanin yields of purple-fleshed sweet potato grown in the northern latitudes and reveal the large diversity of anthocyanins and flavonoids in this popular crop.
Pelargonidin
Peonidin
Anthocyanidins
Anthocyanidins
Cite
Citations (0)
The aim of the present work is to characterize the pigments present in the kernel of four native maize varieties related to the races Arrocillo, Cónico, Peruano, and Purepecha to determine their possible use as natural dyes. Total anthocyanin content is determined by a conventional spectrophotometric method, and anthocyanin analysis is done by high-performance liquid chromatography. The stability of the pigment at pH is also evaluated. The four maize samples contained anthocyanin in both the pericarp and aleurone layer. Total anthocyanin content among samples ranged from 54 mg/100 g of sample to 115 mg/100 g of sample. Anthocyanin profiles are almost the same among the four samples. Differences are observed only in the relative percentage of each anthocyanin. The anthocyanins identified are cyanidin-3-glucoside, pelargonidin- 3-glucoside, peonidin-3-glucoside, cyanidin-3-(6" malonylglucoside), and cyanidin-3-(3",6"-dimalonylglucoside). Anthocyanin extracts showed similar behavior in solutions with different pH. From pH 1-6 lambdamax values are maintained almost constant; however, above this pH value, a marked increase is observed in the bathochromic shifts, but the bluish color did not continue to change above pH 8.
Pelargonidin
Anthocyanidins
Bathochromic shift
Peonidin
Cite
Citations (165)
Pomegranate (Punica granatum L., Punicaceae) fruit is traditionally consumed in several countries, especially in Middle East, and has gained increasing popularity all over the world due to its assumed health benefits. Juices derived from the arils of the seeds were shown to be rich in anthocyanin glucosides, typically composed of cyanidin, delphinidin, and pelargonidin. The aim of the present study was the characterization of diverse Sicilian and Spanish pomegranate accessions regarding their anthocyanin and total polyphenol contents using the Folin-Ciocalteu reagent. The anthocyanin profiles were determined by HPLC-DAD and LC-MS, and color parameters were characterized using the CIELAB coordinates. Antioxidant activities were measured using a fluorimetric assay, and expressed as ORAC values. The anthocyanin and polyphenol contents were correlated with their antioxidant activities. Results obtained were correlated and evaluated for the identification of the most suitable accessions to be selected for cultivation, juice processing, and further breeding.
Punica
Aril
Pelargonidin
Anthocyanidins
Anthocyanidins
Cite
Citations (25)
Petunidin
Pectin
Pelargonidin
Anthocyanidins
Anthocyanidins
Peonidin
Cite
Citations (114)
Pelargonidin
Anthocyanidins
Aglycone
Peonidin
Cite
Citations (458)