Ultrasound-Assisted Extraction, LC–MS/MS Analysis, Anticholinesterase, and Antioxidant Activities of Valuable Natural Metabolites from Astragalus armatus Willd.: In Silico Molecular Docking and In Vitro Enzymatic Studies
Sabrina LekmineSamira BendjedidOuided BenslamaA. I. Martín-GarcíaSamira BoussekineKenza KadiSalah AkkalGema NietoRokayya SamiAmina A. M. Al-MushhinMorooj M. BaakdahAbeer M. AljaadiSaif A. Alharthy
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The Astragalus armatus Willd. plant's phenolic constituent extraction and identification were optimized using the ultrasound-assisted extraction (UAE) method and the LC-MS/MS analysis, respectively. Additionally, cupric reducing antioxidant capacity (CUPRAC), beta carotene, reducing power, DMSO alcalin, silver nanoparticle (SNP)-based method, phenanthroline, and hydroxyl radical tests were utilized to assess the extract's antioxidant capacity, while the neuroprotective effect was examined in vitro against acetylcholinesterase enzyme. This study accurately estimated the chemical bonding between the identified phenolic molecules derived from LC-MS/MS and the AChE. The extract was found to contain sixteen phenolic substances, and rosmarinic, protocatechuic, and chlorogenic acids, as well as 4-hydroxybenzoic, hyperoside, and hesperidin, were the most abundant substances in the extract. In all antioxidant experiments, the plant extract demonstrated strong antioxidant activity and a significant inhibitory impact against AChE (40.25 ± 1.41 μg/mL). According to molecular docking affinity to the enzyme AChE, the top-five molecules were found to be luteolin, quercetin, naringenin, rosmarinic acid, and kaempferol. Furthermore, these tested polyphenols satisfy the essential requirements for drug-like characteristics and Lipinski's rule of five. These results highlight the significance of the A. armatus plant in cosmetics, as food additives, and in the pharmaceutical industry due to its rosmarinic and chlorogenic acid content.Keywords:
Rosmarinic acid
Hyperoside
Chlorogenic Acid
Quercitrin
Leukaemia is a hematopoietic cancer and the treatment has many side effects. For this reason, herbal therapy is an alternative treatment for leukaemia to reduce the side effects. The medicinal herb Plucao (Houttuynia cordata Thunb.) has a vary biological activities including anti-cancer, anti-leukemic, anti-microbial, anti-oxidant, anti-inflammatory and immunomodulatory effects. The active components in Plucao are alkaloids and flavonoids. Quercetin, quercitrin and hyperoside are flavonoid and flavonoid glycosides founded in Plucao. In this study, we extracted Plucao by both distilled deionized water and ethanol. We found that H.cordata ethanolic extract had total flavonoid content more than H.cordata water extract. Total flavonoid of H.cordata ethanolic extract was 224.90 ± 2.80 mg Quercitrin Equivalent (QE)/g dried H.cordata while total flavonoid of H.cordata water extract was 130.79 ± 4.89 mg QE/g dried H.cordata. Moreover, H.cordata extract, quercetin, quercitrin and hyperoside did inhibit the proliferation of Jurkat and U937 leukemic cell lines. The half maximal inhibitory concentration (IC50) values of H.cordata ethanolic extract, H.cordata water extract quercetin, quercitrin and hyperoside on Jurkat were 403.30 ± 5.35, 445.10 ± 6.49, 68.50 ± 2.86, 73.10 ± 2.59, and 75.60 ± 2.25 mg/ml, respectively while IC50 values of H.cordata ethanolic extract, H.cordata water extract quercetin, quercitrin and hyperoside on U937 were 386.20 ± 5.28, 416.40 ± 6.78, 60.50 ± 2.95, 70.10 ± 3.01 and 75.10 ± 1.77 mg/ml, respectively. In addition, our cytokines study found that H.cordata extract and flavonoids especially H.cordata ethanolic extract and quercetin substantially reduced the hematopoietic (IL-2, G-CSF and GM-CSF) and inflammatory (IL-6, IL-10, IL-13, IL-17, IFN-γ, MIP-1β, TNF-α) cytokines. Therefore, we conclude that H.cordata has anti-leukemic and anti-inflammatory activities and may be a potential herbal treatment for leukaemia in complementary medicine.
Quercitrin
Hyperoside
Houttuynia cordata
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Mimosa, an excellent energy crop candidate because of its high growth yield, also contains, on a dry basis, 0.83% hyperoside and 0.90% quercitrin. Hyperoside has been documented as having anti-inflammatory and diurectic properties, whereas quercitrin may play a role in intestinal repair following chronic mucosal injury. Thus, mimosa might first be extracted for important antioxidant compounds and then used as a feedstock for energy production. This article presents results from studies aimed at determining the effect of three extraction parameters (temperature, solvent composition, and time) on the yield of these important quercetin compounds. Conditions are sought which maximize yield and concentration, whereas complementing subsequent biomass pretreatment, hydrolysis and fermentation.
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Hyperoside
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Aim To determine the contents of Hyperoside,Isoquerditrin and Quercitrin-3'-glucoside in Flos Abelmoschus manihot of different areas.Methods The column was Diamonsil C_(18)(150mm×4.6mm,5μm).The mobile phase was methanol-acetonitrile(10∶1)-0.4% phosphoric acid.The flow was 1.0ml/min.The detection wavelength was 360nm.The column temperature was room temperature.Results The content of Hyperoside in the drug of Jiangyan is 1.12% with RSD 1.54%.The content of Isoquerditrin in the drug of Jiangyan is 0.68% with RSD 1.58%.The content of quercitrin-3'-glucoside in the drug of Jiangyan is 0.59% with RSD 1.47%.The content of Hyperoside in the drug of Xinghua is 0.98% with RSD 1.49%.The content of Isoquerditrin in the drug of Xinghua is 0.60% with RSD1.62%.The content of Quercitrin-3'-glucoside in the drug of Xinghua is 0.52% with RSD1.55%.The content of Hyperoside in the drug of Xingtai is 1.03% with RSD1.72%.The content of Isoquerditrin in the drug of Xingtai is 0.62% with RSD1.84%.The content of Quercitrin-3'-glucoside in the drug of Xingtai is 0.54% with RSD1.62%.Conclusion The contents of Hyperoside,Isoquerditrin and Quercitrin3'-glucoside in the drug of Jiangyan are all the highest,the contents of Hyperoside,Isoquerditrin and Quercitrin-3'-glucoside in the drug of Xinghua are all the lowest.This method is simple,accurate and available for the exploitation and the quality control of the drug in different areas.
Hyperoside
Quercitrin
Glucoside
Adulterant
Garcinia kola
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Objective The purpose is to determine the content of hyperoside and quercitrin in Hout-tuynia cordata. Method The HPLC method was used to determine the content of hyperoside and quercitrin in Houttuynia cordata. The analysis was carried out on Shimadzu C18 column(150 mm×4. 6 mm,5 μm). The mobile phase was MeOH-0. 2%H3PO4 (45:55). Flow-rate was1. 0 mL. min-1. Wave-length was 350 nm. Temperature was room temperature. Result The method was simple and had a good linear relationship. The liner range of hyperoside is 0. 50-2. 50 μg and gave a correlation (r) of 0. 999 8. The recovery was 102. 47%. RSD was 1. 04%. The liner range of quercitrin is 0. 40-2. 00 μg and gave a correlation (r) of 0. 999 6. The recovery was 97. 92%. RSD was 2. 13%. Conclusion The method was easy to determine the content of hyperoside and quercitrin in Houttuynia cordata.
Quercitrin
Hyperoside
Houttuynia cordata
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The 74 samples of six HYPERICUM species (H. PERFOROTUM, H. HIRSUTUM, H. MACULATUM, H. TETRAPTERUM, H. MONTANUM, AND H. HUMIFUSUM) were collected around Slovenia and analysed for the content of ten substances (rutin, hyperoside, isoquercetin, quercitrin, quercetin, I3,II8-biapigenin, amentoflavone, pseudohypericin, hypericin, and hyperforin). The flowers were analysed separately from the green parts of the plants (herbs). The highest content of most of the substances was found in the flowers of H. PERFOROTUM. Among the herbal samples (without flowers), H. MONTANUM and H. HIRSUTUM contained significantly higher levels of amentoflavone (average 3-fold and 2.5-fold higher, respectively), than the herbs of H. PERFOROTUM. In the herbal part of H. PERFOROTUM the contents of all constituents strongly correlate with the contents of the same compound in flowers, except for the content of amentoflavone, which is independent in these two parts. Rutin and hyperoside are in positive correlation, and quercitrin is in negative correlation with the altitude of the growing site.
Hyperoside
Amentoflavone
Quercitrin
Hyperforin
Phytochemical
Hypericin
Hypericum
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Quercitrin
Hyperoside
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Quercitrin
Hyperoside
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OBJECTIVE To determine the content of hyperoside and quercitrin in the seeds of Cuscuta chinensis. METHODS The HPLC method was used to determine the content of hyperoside and quercitrin from seeds of Cuscuta chinensic . The analysis was carried out on Shimadzu C_~18 column(150 mm×4.6 mm,5 μm), with MeOH-0.05%H_3PO_4 (45∶55) as mobile phase.flow-rate of 1.0 ml·min~-1 and wave-length at 350 nm.at room temperature. RESULTS The liner range of hyperoside and quercitrin were 0.50-2.50 μg and 0.40-2.00 μg,with a correlation(r) of 0.9998 and 0.9996.The recovery was 95.90% with RSD of 0.93%. The liner range of quercitrin was 0.40-2.00 μg (r=0.9996). The recovery was 99.19% with RSD of 2.39%. CONCLUSION The method is easy to determine the content of hyperoside and quercitrin from seeds of Cuscuta chinensic.
Quercitrin
Hyperoside
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ObjectiveThe purpose is to determine the content of hyperoside,quercitrin and luteolin in polygonum aviculare by HPLC.MethodsThe analysis of hyperoside and quercitrin was carried out on Shimadzu C_(18)column(150 mm×4.6 mm,5 μm).The mobile phase was methanol-0.2%phosphate(45∶55).The flow-rate was 1.0 ml·min~(-1)and wave-length was 350 nm.Temperature was room temperature.The analysis of luteolin was carried out on Shimadzu C_(18)column(150 mm×4.6 mm,5 μm).The mobile phase was methanol-0.2% phosphate(50∶50).The flow-rate was 1.0 ml·min~(-1)and wave-length was 254 nm.Temperature was room temperature.ResultsThe method was simple and had a good linear relationship.The linear range of hyperoside was 0.50~2.50 μg and gave a correlation(r) of 0.999 8.The recovery was 95.13%.RSD was 2.91%.The linear range of quercitrin was(0.40)~2.00 μg and gave a correlation(r) of 0.999 6.The recovery was 96.49%.RSD was 2.77%.The linear range of luteolin was(0.40)~2.00 μg and gave a correlation(r) of 0.999 6.The recovery was 95.75%,RSD 2.49%.ConclusionThe method is easy and fast to determine the content of hyperoside,quercitrin and luteolin from polygonum aviculare.
Quercitrin
Hyperoside
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Eight compounds were isolated from the stem bark of Cornus walteri. On the basis of chemical and spectroscopic evidences, the structures of these compounds were identified as gallic acid, (+)-gallocatechin, (+)-catechin, quercetin, quercitrin, rutin, isoquercitrin and hyperoside respectively.
Quercitrin
Hyperoside
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