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    Factors Affecting the Use of Lactate Dehydrogenase as a Means of Bloodstain Differentiation
    Journal of Forensic Sciences (1978)
    PM StombaughJames Kearney
    Abstract Lactate dehydrogenase (LDH), a ubiquitous enzyme present in human tissues and body fluids, catalyzes the reversible oxidation of lactate to pyruvate. Electrophoretically, LDH can be separated into five distinct isozyme fractions commonly designated as LDH-1, LDH-2, LDH-3, LDH-4, and LDH-5 [1].
    L-Lactate dehydrogenase
    Citations (9)
    [A study of lactate dehydrogenase isozyme from the corneal epithelium. I. Lactate dehydrogenase isozyme patterns of various species of animals under conditions of various developmental and regenerative stages].
    PubMed (1967)
    K Shiba
    L-Lactate dehydrogenase
    Citations (0)
    [On the behaviour of lactate-dehydrogenase (LDH)-isoenzymes in the serum of lathyritic rats].
    PubMed (1969)
    H. HolzmannB. MorschesF Lelaona
    L-Lactate dehydrogenase
    Citations (0)
    The effects of intermittent training and age on blood lactate (La) Level and lactate dehydrogenase enzyme (LDH) activity in male wistar rats.
    Pars of Jahrom University of Medical Sciences (2014)
    Hamid MohebiFarhad RahmaniniyaAllahyar ArabmomeniAhmad ReyasiMohammad Marandi
    Introduction: Physical activity and age are among the factors affecting lactate levels and lactate dehydrogenase activity. Physical activity appears to be able to counterbalance the morphological and metabolic changes associated with aging that decrease physical ability and performance. Objectives: The purpose of the present study was to assess the effects of interval training on blood lactate levels and lactate dehydrogenase activity in young and old rats. Materials and Methods: A total of 40 male rats were selected and then divided into two age groups -the old group (20 rats aged 27 months and weighing 389±31 g) and the young group (20 rats aged 3 months and weighing 224±13 g). Each group was itself randomly divided into an experimental group (n=10) and a control group (n=10). The training protocol involved 4 minutes of running on the treadmill with intervals of 2 minutes active resting in 10 training bouts of 60 minutes, for 6 sessions per week and for 8 weeks and gradually increasing in intensity. Twenty-four hours after the last training session, the rats were anesthetized with a mixture of ketamine and xylazine in order for their blood sample to be collected from their cardiac puncture. Their lactate levels and LDH activity were then measured by an enzymatic method. Data were analyzed using the one-way ANOVA and Tukey's post-hoc test. Results: The results showed no significant differences in blood lactate levels between the four groups; however, LDH activity was significantly higher in the young experimental group than in the young control group (p<0/05). Conclusion: The results indicate that lactate is increasingly cleared by interval training. It also appears that the effect of training on lactate clearance is similar in both young age and old age. Monitoring blood lactate levels benefits muscle glycogen replenishment and intracellular pH (pHi) regulation.
    L-Lactate dehydrogenase
    Citations (5)
    Lactate Dehydrogenase Isoenzymes
    Clinical Chemistry (1967)
    Arthur F. KriegLawrence RosenblumJohn B. Henry
    Abstract Five lactate dehydrogenase isoenzymes are normally observed in human serum: H4 (LDH1), H3M (LDH2), H2M2 (LDH3), HM3 (LDH4), and M4 (LDH5). This study presents evidence that these isoenzymes vary in the ratio of LDH activity (pyruvate → lactate) to LDH activity (lactate → pyruvate).
    L-Lactate dehydrogenase
    Citations (66)
    Characteristics of membrane-bound pool of leukocyte lactate dehydrogenase in healthy men of different age
    Human Physiology (2015)
    Olga FedorenkoН. А. БоханSvetlana А. Ivanova
    L-Lactate dehydrogenase
    Human physiology
    Citations (1)
    An evaluation of the effect of haemolysis on the Roche lactate dehydrogenase assay
    Pathology (2023)
    Vraj ShahKevin JessenChris CocotsiAilie Connell
    Haemolysis
    L-Lactate dehydrogenase
    Lactic dehydrogenase
    Citations (0)
    Evidence for the Random Aggregation of Sub-units to produce Multiple Forms of Lactate Dehydrogenase in the Brains of Rat and Man
    Nature (1967)
    R. G. SpectorDavid C. Watts
    L-Lactate dehydrogenase
    Citations (4)
    Some observations on serum lactate dehydrogenase (pyruvate→ lactate) assays optimised at 37°
    Clinica Chimica Acta (1973)
    J F TuckermanA R Henderson
    L-Lactate dehydrogenase
    Citations (4)
    [A study of lactate dehydrogenase isozyme from the corneal epithelium. II. Lactate dehydrogenase isozyme from the bovine corneal epithelium].
    PubMed (1967)
    K Shiba
    L-Lactate dehydrogenase
    Citations (0)