Enhanced Antibacterial Activity of a Cationic Macromolecule by Its Complexation with a Weakly Active Pyrazole Derivative
5
Citation
33
Reference
10
Related Paper
Citation Trend
Abstract:
Molecules containing the pyrazole nucleus are widely reported as promising candidates to develop new antimicrobial compounds against multidrug-resistant (MDR) bacteria, where available antibiotics may fail. Recently, aiming at improving the too-high minimum inhibitory concentrations (MICs) of a pyrazole hydrochloride salt (CB1H), CB1H-loaded nanoparticles (CB1H-P7 NPs) were developed using a potent cationic bactericidal macromolecule (P7) as polymer matrix. Here, CB1H-P7 NPs have been successfully tested on several clinical isolates of Gram-positive and Gram-negative species, including relevant MDR strains. CB1H-P7 NPs displayed very low MICs (0.6-4.8 µM), often two-fold lower than those of P7, on 34 out of 36 isolates tested. Upon complexation, the antibacterial effects of pristine CB1H were improved by 2-16.4-fold, and, unexpectedly, also the already potent antibacterial effects of P7 were 2-8 times improved against most of bacteria tested when complexed with CB1H. Time-killing experiments performed on selected species established that CB1H-P7 NPs were bactericidal againstKeywords:
Pyrazole
We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains: Pseudomonas aeruginosa ( S1 ), Escherichia coli ( S2 ) and Staphylococcus aureus ( S3 ). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with ( S1 ) and ( S3 ). Eugenol reacts positively with the Pseudomonas aeruginosa ( S1 ) at all concentrations and with the Escherichia coli ( S2 ) at 0.5 mg/ml. We remark that the Pseudomonas aeruginosa ( S1 ) is the more sensitive strain than Escherichia coli ( S2 ) and Staphylococcus aureus ( S3 ). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.
Cite
Citations (1)
We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains: Pseudomonas aeruginosa ( S1 ), Escherichia coli ( S2 ) and Staphylococcus aureus ( S3 ). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with ( S1 ) and ( S3 ). Eugenol reacts positively with the Pseudomonas aeruginosa ( S1 ) at all concentrations and with the Escherichia coli ( S2 ) at 0.5 mg/ml. We remark that the Pseudomonas aeruginosa ( S1 ) is the more sensitive strain than Escherichia coli ( S2 ) and Staphylococcus aureus ( S3 ). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.
Cite
Citations (0)
Mupirocin is a topically and short-term used antibiotic, known for its antimicrobial activity against staphylococci, streptococci and some Gram-negative bacteria. Levomenthol is a plant derived cyclic alcohol which possesses antimicrobial activity. The aim was to study the antimicrobial activity of single mupirocin and levomenthol in vitro as well as their mixture towards planktonic bacteria and biofilm built by Staphylococcus aureus ATCC 29213 and five MRSA clinical isolates. Minimal inhibitory concentrations (MICs) of both compounds were determined using broth microdilution method according to CLSI guidelines with breakpoints determined using TTC reduction assay (1). Mupirocin was effective against all tested strains and MICs were between 0.125 and 0.250 μg/mL. MICs obtained for levomenthol was much higher, between 0.268 and 1.933 mg/mL. When both compounds were applied simultaneously, the antagonistic activity was recorded. However, when mupirocin was applied 1 hour after levomenthol treated S. aureus, MICs of both compounds for the majority of tested strains were lower. Biofilm inhibitory concentrations (BICs) were estimated by the MTT assay according to Walnecka et al (2). Both mupirocin and levomenthol applied alone showed antibiofilm activity in concentrations above 1 mg/mL, while their mixture exert stronger activity suggesting that synergistic or at least additive interactions might be implicated.
Mupirocin
Broth microdilution
Cite
Citations (0)
Abstract Antibacterial activity of ethanolic extract of Argemone mexicana seeds was assessed against Escherichia coli and Staphylococcus aureus by disc-diffusion method. The extract had significant inhibitory effect against Staphylococcus aureus and Escherichia coli with Minimum Inhibition Concentration values of 3 mg/ml and 3.5 mg/ml respectively. The phytochemical analysis of A. mexicana seeds demonstrated that the secondary metabolites such as tannins, flavonoids and steroids were present. The antibacterial activity of A. mexicana seeds extract may be due to such secondary metabolites.
Phytochemical
Escherichia
Agar diffusion test
Cite
Citations (0)
Infectious disease is the biggest problem in the world. The problem with this infection is related to antibiotic resistance if its use is not consistent. Utilization of kemangi leaves, which are always used as fresh vegetables, has potential as an antibacterial in inhibiting the growth of Escherichia coli and Pseudomonas aeruginosa bacteria. This study aims to analyze the inhibition of an ethanol extract of kemangi leaves against Escherichia coli and Pseudomonas aeruginosa. The Kirby-Bauer method with the disc diffusion method was used to determine the minimum inhibitory diameter and to calculate the activity index value. The test results at a concentration of 500 mg/mL showed activity in the strong category, namely 13.70 ± 0.10 mm (Escherichia coli) and 12.93 ± 0.06 mm (Pseudomonas aeruginosa), and the minimum inhibitory concentration was at a concentration of 3.125 mg/mL. Conclusion the ethanol extract of kemangi leaves shows antibacterial activity.
Agar diffusion test
Minimum bactericidal concentration
Cite
Citations (1)
Objective To evaluate the in vitro inhibitory activity of antibacterial material of Pseudomonas aeruginosa against methicillin-resistant Staphylococcus aureus(MRSA).Methods In vitro inhibitory activity of antibacterial material of Pseudomonas aeruginosa against 32 strains of MRSA was determined by using cross streak assay.Results In vitro inhibitory activity of antibacterial material of Pseudomonas aeruginosa against MRSA appeared satisfactory.Of the 15 strains of Pseudomonas aeruginosa,7 strains(produced blue-green pigment) showed 100% inhibition rate against MRSA,with the average diameter of inhibition zone of 37.7 mm.The antibacterial activity of pigment-producing strains was better than that of non-pigment producing strains.Conclusion The antibacterial material of Pseudomonas aeruginosa has strong antibacterial activity against the 32 strains of MRSA.This finding,which is the first reported in Chine,will pave a new way for the development of antibacterial drugs against MRSA infection.
Cite
Citations (0)
Nine 3-acetylcoumarins derivatives were synthesized and characterized via MS, 1 H NMR, FT-IR, and UV-vis absorption spectra. The fluorescence behaviors of the compounds in dichloromethane solutions were observed. The minimum inhibitory concentration was observed for the nine title compounds and antibiotics, to evaluate their in vitro antibacterial activity against Staphylococcus aureus (S. aureus ATCC 29213), methicillin-resistant S. aureus (MRSA XJ 75302), Escherichia coli (E. Coli ATCC 25922), and extended spectrum b lactamases E. coli (ESBLs-E. coli ATCC 35218). These results showed that the compound 2 exhibits the antibacterial activities to S. aureus.
Dichloromethane
Cite
Citations (0)
Cite
Citations (4)
Cationic polymerization
Quinine
Cite
Citations (14)
The cytotoxicity and antimicrobial activity of goniothalamin isolated from Bryonopsis laciniosa was investigated. Goniothalamin showed potent cytotoxicity with LC50 values (5.03 microg/mL) comparable with the reference standard agent, gallic acid. It also showed weak antibacterial and significant antifungal activity against a wide range of gram positive and gram negative bacteria and fungi. The antibacterial (minimum inhibitory concentration) effect against Bacillus cereus and Shigella shiga was found to be 64 microg/mL.
Gram-Positive Bacteria
Gram-Negative Bacteria
Cite
Citations (68)