Changes in frequencies of clonable pre B cells during life in different lymphoid organs of mice
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Precursor cell
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Objective:The spleen-deficiency-suffering mice models are observed dynamically on the perspective of pharmacokinetics and by radioactive tracer method,and the effect of spleen-reinforcing therapy on the mice's obsorption of Fe and the process of transporting and distribution is observed as well,so that the relationship between the spleen's function of dominating transporting/ dysfunction of the spleen in transporting and Fe metabolim is researched; The biological meaning of spleen's function of dominating transporting is discussed;The scientific content of the important TCM theories of spleen's dominating the transporting and spleen's determining the condition of the acquired construction is revealed;the experimental basis is provided for the research on spleen's functions and /or the essence of spleen-deficiency syndrome.Method: 80 Male SD mice are divided by random into normal group,normal reinforcing-spleen group,spleen-deficiency group,reinforcing-spleen-deficiency group.The mice model are fed 59 Fe Cl3 and 0.2 ml water-floating fluid at one time;the mice of the groups of normal reinforcing-spleen and reinforcing-spleen-deficiency are fed 1.5ml 1:1 Si Jun Zi Tang;the groups of normal and spleen-deficiency are fed 1.5 ml normal sanile once a day.3 rats of each group will be selected by random at 0.5,1,2,4,8 and 14 phase and are made dead by eyeball blood letting method,and then radio 59 Fe level is measured by Y radio-immunity counter after the organs and tissues of spleen,liver,small intestine,stomach and kidney are extracted,and the pharmacokinetics change of radio Fe's transporting and distribution from the whole body's circulatory system to the rats's tissues and organs.Results: 59 Fe transporting exists significant difference in the condition of spleen-deficiency and reinforcing-spleen,the therapy of reinforcing-spleen improve the transporting and distribution of 59 Fe of the mice.Conclusions: 59 Fe's transporting process from blood circulatory system to the organs and tissues decreased in the condition of spleen-deficiency,the therapy of reinforcing-spleen can promote the transporting of 59 Fe in the tissues and organs of the mice,especially concentrating on the spleen,which shows that Si Jun Zi Tang has the function of regulating the spleen-deficiency by reinforcing the spleen so that the substance transporting system of the organism is regulated.
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Single 1-day-old C3H spleen grafts in adult splenectomised (AKR X C3H)F1 mice grew to a larger size and contained more lymphoid tissue than grafts in sham-operated mice. In splenectomised hosts, individual spleen grafts attained a progressively smaller size in hosts grafted with 6 and 12 spleen grafts per animal. Thus the total mass of spleen graft tissue in such animals reached a plateau which approximated the weight of the normal spleen in such animals. Similar results were obtained with AKR spleen grafts in AKR mice and with C57BL spleen grafts in (AKR X C57BL) F1 hybrid mice. In sham-operated hosts a similar tendency for a reduction in individual spleen graft size after multiple grafting was also noted. Total spleen mass appears to be under positive regulation by the body but the mechanisms involved are unknown.
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The influence of the spleen on the distribution of blood neutrophils was studied in rats with varying spleen sizes following transfusion of isologous 3 H‐dT‐labelled neutrophils. The weight of the spleen correlated with the splenic neutrophil pool (r = 0.87, P < 0.01). Alterations in spleen size did not significantly change the total blood neutrophil pool, but with increasing splenomegaly circulating and marginal neutrophils were shifted into the splenic pool. With massive splenomegaly, up to 50% of the total blood neutrophils were trapped in the spleen. These findings suggest splenic pooling of neutrophils and a shift of circulating and marginal neutrophils into the spleen proportionate to the splenic tissue present.
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Objective:On basement of model of rat' deficient spleen syndrome,these are the dynamic observation studies of?~(59) Fe's obsorbed metabolism and /or transported metabolism course of the rat of deficient spleen syndrome and replenishing spleen method by the application of radioactivity label technology in pharmacokinetics.The experiment studies the relation of the spleen being the transferring owner or the spleen losing the transferring owner with Fe's metabolism,researches it's biology meaning of spleen being the owner of transmission,researches the science intension of important traditional Chinese medicine theory of spleen being the owner of transmission and the spleen serving as basement of the day after tomorrow,and provides the experiment basis of spleen merit ability and/or essece of deficient spleen syndrome.Methods:40 male SD rats were randomly divided into 4 groups:normal group,normal replenishing spleen group,deficient spleen group,deficient spleen-replenishing spleen group.According to the requirement of the animal model standardizes most of rats were made the pattern.4 goups were once iffigated shui xuanye of?~(59) FeCl_3 0.2ml/220g/a rat.At the same time,according to weight of a person and a rat,2 groups of normal replenishing spleen group and deficient spleen-replenishing spleen group were given 1.5m±medicinal ingredients liquid of 1:1 four-man decoction,the others were given 1.5m±physiological saline,once a day.Respectively at 0.5h,1h,1.5h,3h,6h,12h and 24h,blood 0.2ml of all rats were taken from rat's tail,measured its radioactivity?~(59) Fe with γ-radiation immunity counter,to observe changes of pharmacokinetics of?~(59) Fe passing through rat's stomach and bowel way into the whole body blood-system.Results:The experiment exists the clear difference for changes of radioactivity?~(59) Fe pharmacokinetics in the states of deficient spleen and replenishing spleen to absorb?~(59) FeCl_3 from rat's stomach and bowel way into the whole body blood-system.Replenishing spleen method have promoted the absorb to?~(59) FeCl_3.Conclusions:The rat of deficient spleen syndrome has reduced the bioavailability of?~(59) FeCl_3 and made it the lower lever to absorb of?~(59) FeCl_3.On the contrary,Replenishing spleen method has promoted to absorb?~(59)(FeCl_3).from rat's stomach and bowel way into the whole body blood-system.
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Objective To observe and explore the effect of activating the spleen for the immune function of spleen deficiency rat.Methods The priscription of Yunpi is composed of Huangqi,Danggui,Cangzhu,Longgu and so on.Reserpine was used to duplicate spleen deficiency model.We divided the rats into three groups:normal grouop,model group and therapy group to observe the effect of activating the spleen for the tissue structure of main immune organ such as spleen and thymus,serum IgG,IgA,IgM,SOD.Results Activating the spleen could significant repair the tissue structure of thymus and improve Ig,SOD.Conclusion Activating the spleen could significant improve immune function of spleen deficiency rat by improving SOD,Ig and repairing the tissue structure of thymus.
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Neoplasm
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The author studied the ability of stromal precursor cells of mouse bone marrow to intracellular repair of radiation-induced injuries. It was shown that the slow-restorable, highly radioresistant population of stromal precursor cells is capable of early post-radiation recovery.
Radioresistance
Precursor cell
Radiosensitivity
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Precursor cell
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