Persistence of the same viral strain in early and late relapses of Epstein-Barr virus-associated Hodgkin's disease
Pierre BroussetD SchlaiferF MeggettoElias BachmannSylvia RothenbergerJ PrisGeorges DelsolHans Knecht
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clone (Java method)
Southern blot
Southern blot
Dot blot
Oligomer restriction
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clone (Java method)
Cytomegalovirus
Immunofluorescence
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The clone KB-230 of simian adenovirus SA7(C8) is described differing from the reference SA7(C8) strain and clones KB-2 and MB-1 by the presence of additional recognition sites when treated with different endonucleases. The KB-230 clone differs antigenically in the neutralization test from the MB-1 clone. Some biological and molecular-biological properties of the KB-230 clone were studied. All the simian cell cultures under study were highly sensitive to the cytopathic effect and replication of the KB-230 clone. The reproduction cycle of the KB-230 clone was 12 h, that of KB-2 clone 16 h. The maximum accumulation of virus in the cells was observed by 27-29 h for both clones. The KB-230 clone differed from the KB-2 clone by early development of the CPE (by 9 h of cultivation against 19 h for the latter). The oncogenic activity of the KB-230 clone was less marked than that of the MB-1 clone. The methods of heteroduplex and polypeptide analysis established the difference of the KB-230 clone from the reference SA7(C8) strain and KB-2 and MB-1 clones.
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Differences of host immune response and of chemosensitivity between two clones derived from a methylcholanthrene-induced rat fibrosarcoma and exhibiting different tumorigenic and metastatic potentials were examined. Clone G, which has no metastatic potential, was more sensitive to natural killer cytotoxicity by syngeneic rat spleen cells than clone A, which is highly metastatic. The colony formation of clone G was strongly inhibited by the supernatant obtained from coculture of lymphocytes with either of these two clones. In contrast, the colony formation of clone A was not inhibited by these supernatants. Inhibition of the colony formation of clone G was dependent on the dilution of the supernatant and the incubation time of the lymphocytes and tumor cells used to generate the supernatant. The supernatant from cocultures of lymphocytes and clone G inhibited the colony formation of clone G more strongly than the supernatant from cocultures of lymphocytes and clone A. There was no difference between these two clones in chemosensitivity to seven of the ten chemotherapeutic drugs tested.
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The afforestation experiment of 21 poplar clones was conducted in Yiling District of Yichang City located in the western mountain areas of Hubei.The results showed that the five clones including clone 'Lushan',clone 'M81-18',clone '351',clone '375' and clone 'Zhonghan-17' were of excellent characteristics in growth and stable in the areas and not only suitable for intensive short-rotation plantation but also suitable for Short-rotation plantation.However,7 clones of 21 clones including clone 'Zhongjia-8',clone 'Zhongjia-5',clone 'Zhongjian-1',clone 'Zhongjian-3',clone 'Tianyan',clone 'Liaohe' and clone '108' were unsuitable for their lower survival rate and slow growth.The results provided a good basis for the selection of superior poplar clones in the western mountain areas of the Hubei.
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To establish human embryonic stem cells (hESCs) feeder-independent and cell factor-free culture system.Effect of high and low clone densities of hESCs culture was compared and impact of the clone densities to hESCs culture media was analyzed.HESCs could maintain their undifferentiated states at high clone density (34 clones/cm²) without cell factors. At the same time, the bone morphology protein (BMP)-like induction of N2 and B27 supplements (NB) medium could be modulated by the clone density, and high level of BMP-like induction was accompanied by high clone density.High clone density of hESCs can change the environments by themselves to maintain the undifferentiated states, which provides a new clue to explore the mechanism of undifferentiated states of hESCs and simplify the culture medium.
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Abstract Monoclonal gammopathy of renal significance (MGRS) encompasses a group of disorders in which a monoclonal immunoglobulin (M-protein) secreted by a B-cell or plasma cell clone causes renal disease. Proliferative glomerulonephritis with monoclonal immunoglobulin deposits (PGNMID) is a form of MGRS where M-protein is deposited in the glomerulus. Although evidence is limited, the current consensus is that therapy for PGNMID should be directed against the underlying clone. However, it is conceivable that there is heterogeneity in the renal prognosis of PGNMID and that not all patients have need for clone-directed therapy. Here, we report two cases of PGNMID with IgM-kappa gammopathy. In one case of a 53-year-old woman the glomerulonephritis resolved without clone-directed therapy. In the other case of a 34-year-old woman clone-directed therapy was discontinued due to adverse effects. Although no hematological response was achieved, the PGNMID resolved. In both cases there are no signs of a recurrent glomerulonephritis in over 3 years of follow-up. Here, we review the literature and suggest that some PGNMID patients have a favorable renal prognosis in whom clone-directed therapy can be withheld or postponed. Further research is warranted to yield predictors to identify these patients and to better understand the disease course of PGNMID.
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Gammopathy
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Dialectic
SWORD
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Abstract A T cell helper clone was derived 2 yr ago from a mixed lymphocyte culture. This clone, referred to as clone 9, was propagated in interleukin 2 (IL 2)-containing medium in the presence of irradiated stimulator and irradiated syngeneic spleen cells. Clone 9 was of H-2d origin and was found to be Thy-1+ and Lyt-1-2-. Clone 9, as well as supernatant factor(s) derived from it, were able to enhance the primary cytotoxic responses of Db responder cells to alloantigens. Furthermore, clone 9 cells or its factor(s) were only active when added during the first 24 hr of a 5-day culture period. When a low stimulator cell dose (10(4) cells per 0.2 ml culture) was used, it was possible to demonstrate that clone 9 also required a source of irradiated allogeneic splenic accessory cells to exert its helper action. Under these conditions, clone 9 or its factor(s) could also synergize with IL 2-containing medium in mounting cytotoxic responses to alloantigens. Synergy between IL 2-containing medium and clone 9 or its factor(s) was observed only when Db responder cells were used. The helper activity in clone 9 supernatant was also specifically absorbed out by Con A-stimulated Db spleen cell blasts. Preincubation with clone 9 supernatant for 1 hr at room temperature also led to enhanced cytotoxic responses of Db responder cells to alloantigens. Clone 9 supernatant was also found to be devoid of detectable IL 2 activity. Thus, clone 9 or its helper factor(s) appear to exert its helper activity by an early interaction with Db cytotoxic T lymphocyte precursors (CTL-P).
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