Possible Association between Selected Tick-Borne Pathogen Prevalence and Rhipicephalus sanguineus sensu lato Infestation in Dogs from Juarez City (Chihuahua), Northwest Mexico–US Border
Diana M. Beristain-RuizJavier A. Garza‐HernándezJulio Vicente Figueroa MillánJosé J. Lira-AmayaAndrés Quezada‐CasasolaSusana Ordoñez-LópezS. Viridiana Laredo-TiscareñoBeatriz Alvarado-RoblesOliver R. Castillo-LunaAdriana Floriano-LópezLuis M. Hernández‐TrianaFrancisco Martínez-IbáñezRamón Rivera-BarrenoCarlos A. Rodríguez-Alarcón
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Abstract:
Tick-borne bacterial pathogens (TBBPs) show a worldwide distribution and represent a great impact on public health. The brown dog tick (Rhipicephalus sanguineus) is a vector of several pathogens that affect dogs and sometimes humans as well. In addition, TBBPs represent a diagnostic challenge and imply financial resources and medical treatment for long periods of time. In the present study, R. sanguineus s. l. was identified as the main tick species naturally parasitizing dogs that inhabit. Juárez City, Chihuahua, in the Paso del Norte region, Mexico-US Border, representing 99.8% of the cases. Additionally, an end-point PCR was performed to search for whether pathogens in R. sanguineus s. l. can transmit in DNA extracted from ticks and dog blood samples. This is the first molecular detection of Rickettsia rickettsi infecting domestic dogs in Mexico; however, other pathogens were also identified, such as Ehrlichia canis and Anaplasma platys in both ticks and dog blood samples, while Anaplasma phagocytophilum was identified only in dog blood samples. Moreover, co-detection in tick pools and co-infection in the analyzed dog blood samples could be found. Similarly, this research showed that dogs were found mostly parasitized by adult female ticks, increasing the possibility of transmission of E. canis.Keywords:
Rhipicephalus sanguineus
Ehrlichia canis
Anaplasma phagocytophilum
Tick infestation
Rhipicephalus
Babesia canis
Tick-borne pathogens (TBPs) are of great concern having the potential to threaten canine health. Dogs infected with Ehrlichia canis, Anaplasma platys, Babesia canis, and Hepatozoon canis are commonly found in Thailand; Rhipicephalus sanguineus tick is the most common vector of diseases. This study aimed to determine the prevalence of common TBPs in dogs and their ticks in Thailand using polymerase chain reaction (PCR) and DNA sequencing methods.Forty-four blood samples were positively diagnosed with TBPs infection by microscopy. Samples were from animal hospitals in Maha Sarakham, Amnat Charoen, Nakhon Ratchasima, and Bangkok, Thailand, during January-June 2020. Five to six ticks were also taken from infected dogs, and then, both blood and tick were analyzed using PCR and DNA sequencing.PCR results showed that R. sanguineus was the only tick species detected in this study. The appearance of single infection with E. canis was the most common infection found in dogs and ticks (64% and 82%, respectively). Correlation of pathogen infection in hosts and their vector was performed by similarity detection of pathogens between blood and tick samples based on PCR analysis in 29 samples (66%) but there was no significant differentiation.E. canis appears as the most common canine tick-borne pathogen in Thailand, which was detected in both healthy and sick dogs as well as in R. sanguineus. The findings show the relationships among host dogs, pathogens, and ticks. Veterinarians should be proactive in educating pet owners about the risks associated with ticks and their important pathogens and plan effective control strategies.
Rhipicephalus sanguineus
Ehrlichia canis
Tick infestation
Babesia canis
Hepatozoon
Tick-borne disease
Ehrlichiosis
Bartonella
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Rhipicephalus sanguineus
Ehrlichia canis
Babesia canis
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Hepatozoon
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The central region of Brazil is known to be an endemic area for canine ehrlichiosis. Therefore, this study aims to determine the prevalence rates of E. canis infection in dogs and in Rhipicephalus sanguineus ticks collected from the dogs and their home environments. Serum samples and genomic DNA from the blood of 20 dogs and 299 ticks were analyzed by IFA and PCR assays in order to detect Ehrlichia canis antibodies and DNA. Nine (45%) of the 20 dogs were seropositive for E. canis, with titers ranging from 80 to 10240, and 6 dogs (30%) were positive for Ehrlichia spp. by PCR. Five free-living ticks were positive (2.89%, 95% confidence interval: 0.94-6.62%), as were six ticks attached to dogs (4.76%; 95% CI: 1.77-10.0%). The two groups showed a similar infection rate (P=0.395). Partial dsb DNA sequences of two samples from ticks were identical to each other and 100% (350/350 nucleotides) were identical to E. canis. Despite the high serological and molecular rates of canine ehrlichiosis in Cuiabá, the prevalence among infected ticks was lower than that found among dogs. However, adult ticks may remain infective much longer to ensure their infestation and infection of susceptible dogs.
Rhipicephalus sanguineus
Ehrlichia canis
Ehrlichiosis
Tick infestation
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Hemoparasitosis are diseases of great importance in the practice of a small animal’s veterinarian, due to their high number of cases in the clinical routine of these species, and the wide occurrence of their vectors. Related to hemoparasitosis in dogs, the tick Rhipicephalus sanguineus has the greatest importance due to parasitizing dogs, perpetuating hemoparasitosis in this host group. The present work describes a literature review about the three main hemoparasitosis in the routine of a veterinarian in Brazil, ehrlichiosis, caused by Ehrlichia canis, babesiosis caused by the protozoa Babesia canis and Babesia gibsoni, and anaplasmosis caused by Anaplasma platys, comparing them regarding the differences between clinical signs and laboratory alterations, such as the different types of anemia caused by these conditions that culminate in clinical signs related to them. It also aims to describe the similarity in the diagnostic techniques used and infection treatments, which in all cases is used tetracycline, the most commonly used is doxycycline, and highlight the need for further studies about this topic.
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Ehrlichia canis
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Ehrlichia canis
Hepatozoon
Tick infestation
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Rhipicephalus sanguineus ticks are the most important group of arthropod vectors responsible for transmission of Ehrlichia canis and causing canine ehrlichiosis in dogs. In the present study, 20 ticks were collected from 40 dogs in TVCC, LUVAS, Hisar, India. Species identification under a stereoscopic microscope was performed. A total of 17 (42.50%) out of 40 dogs harbored clinical tick infestation. Among dogs with clinical tick infestation, the overall prevalence of Rhipicephalus sanguineus was recorded as 70.58%.
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Ehrlichia canis
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has been the main hemopathogen affecting domestic dogs in Brazil. Even though tick-infested dogs may lead to household infestation and predispose human exposure and public health concern, no comprehensive study has surveyed humans, dogs, and environmental ticks altogether.
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The ability of Frontline Tri-Act®/Frontect®, a topical ectoparasiticide containing fipronil and permethrin for dogs, to prevent the transmission of Babesia canis as well as Ehrlichia canis was evaluated by infesting dogs with infected vector ticks. For the Babesia canis study, 16 dogs were randomly allocated to two groups. Eight dogs were treated on day 0 with a topical spot-on formulation containing 6.76 % w/v fipronil plus 50.48 % w/v permethrin and eight dogs served as the untreated control group. Dermacentor reticulatus ticks, with a B. canis infection rate ranging between 2 and 10 %, were placed onto dogs on days 7, 14, 21 and 28. In situ tick counts were performed on Days 9, 16 and 23. Ticks were counted and removed on Day 30. Infection of the dogs with B. canis was monitored by rectal temperature readings, clinical examinations and blood smears as well as PCR and IFA (indirect fluorescent antibody assay). For the Ehrlichia canis study, another 16 dogs were allocated to two groups. Eight dogs were treated with the fipronil and permethrin combination on days 0 and 28 and eight dogs served as untreated controls. Rhipicephalus sanguineus ticks, carrying an infection rate of 13 % for E. canis, were released in the sleeping kennels of the dogs on days 7, 14, 21, 28, 35, 42, 49 and 56. Ticks were counted in situ on the dogs on a weekly basis. All ticks were removed and counted on the final assessment day 58. Infection of the dogs with E. canis was monitored by rectal temperature, clinical examinations, and testing of blood samples by PCR, IFA and platelet counts. B. canis was transmitted by D. reticulatus ticks to all eight untreated control dogs and to one treated dog, which was confirmed by blood smears, PCR and IFA. E.canis was transmitted by R. sanguineus ticks to all eight untreated control dogs. Two of the dogs in the treated group were found positive based on PCR and/or IFA. Frontline Tri-Act®/Frontect® significantly lowered the risk for dogs to acquire a B. canis infection by 87.5 % over a challenge period of 28 days. The risk for dogs to acquire E. canis was reduced by 75 % over a period of 56 days.
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Ehrlichia canis
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Rhipicephalus sanguineus and Haemaphysalis longicornis ticks were detected from 58 (57.4%) and 2, respectively, of 101 dogs examined in Okinawa Island. Of 170 sera 25 (14.7%) and 3 were positive for antibodies to Ehrlichia canis and Hepatozoon canis, respectively, while 16 (9.4%) had antibody to Babesia gibsoni. On the smear samples of peripheral blood from 164 dogs, there were no infected cells with E. canis or H. canis, while 8 of 164 (4.9%) were shown to be infected with Babesia spp. Microfilarias were seen on the peripheral blood from 35 of 164 (21.3%) dogs, and all of them were morphologically identified as those of mosquito-borne Dirofilaria immitis but not of tick-borne Dipetalonema spp.
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Babesia canis
Ehrlichia canis
Haemaphysalis longicornis
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