Emergence of ST39 carbapenem-resistant Klebsiella pneumoniae producing VIM-1 and KPC-2
Theodoros KarampatakisCharalampos ZarrasStyliani PappaEleni VagdatliΕlias IosifidisEmmanuel RoilidesAnna Papa
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Objective To investigate the distribution of class I integron in Klebsiella pneumoniae isolated clinically, and analyze the impact of the integron on bacterial resistance. Methods Susceptibility? tests were performed on 127 Klebsiella pneumoniae clinical isolates by K-B disc diffusion method. Drug resistance was analyzed by WHONET 5. 6. The class I integron in the 127 Klebsiella pneumoniaes was detected by polymerase chain reaction; Comparison of the resistance was conducted between the class I integron positive strains and negative strains. Results There were 53 strains( 41. 7%) of Klebsiella pneumoniaes containing the class I integron,which showed higher resistance to aminoglycosides,quinolones and most beta lactams than the integron-negative strains. Conclusion Class I integrons are widespread in Klebsiella pneumoniae isolates,which make it easier for them to acquire drug resistance.
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Colistin is one of the antibiotics of last resort for the treatment of carbapenem-resistant Klebsiella pneumoniae infection. This study showed that capsular type K64 (50%) and ST11 (53.9%) are the prevalent capsular and sequence types in the colistin-resistant strains in Taiwan. The interruption of transcripts (38.5%) and amino acid mutation (15.4%) in mgrB are the major mechanisms contributing to colistin resistance. In addition, novel single amino acid changes in MgrB (Stop48Tyr) and PhoQ (Leu26Pro) were observed to contribute to colistin resistance.
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