Inhibition of Vesicle Transport and Phospholipid Excretion into Bile by a Cysteine Protease Inhibitor in the Isolated Perfused Rat Liver

Conference Abstract| April 01 1981 BILE ACIDS PRODUCTION BY ISOLATED RAT HEPATOCYTES H. J. M. Kempen H. J. M. Kempen 1Gaubius Institute, Health Research Organization TOO, Herenstraat 5d, 2313 AD Leiden, The Netherlands Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1981) 9 (2): 212P. https://doi.org/10.1042/bst009212pe Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation H. J. M. Kempen; BILE ACIDS PRODUCTION BY ISOLATED RAT HEPATOCYTES. Biochem Soc Trans 1 April 1981; 9 (2): 212P. doi: https://doi.org/10.1042/bst009212pe Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1981 Biochemical Society1981 Article PDF first page preview Close Modal You do not currently have access to this content.

Icon

Download

Special collections

10.1042/bst009212pe

Cite

Citations (0)

Assay Systems Using Cultured Hepatocytes for Pharmacological and Nutritional Actions of Amino Acids and Related Compounds

Springer eBooks (1991)

Kazumi Yagasaki K. Ishihara Naoko Morisaki Asuka Miura Ryuhei Funabiki

Protein metabolism

10.1007/978-94-011-3550-4_46

Cite

Citations (0)

Effects of inhibitors of lysosomal function on the degradation of 125I-insulin and 125I-asialofetuin by the perfused rat liver and isolated hepatocytes

W. F. Ward Anita L. Moss

Degradation

Cite

Citations (0)

Disposition of Cosalane, a Novel Anti-HIV Agent, in Isolated Perfused Rat Livers

Drug Metabolism and Disposition (1999)

Chandrasekhar Udata Alok K. Mitra Mostafa Z. Badr

Cosalane is a potent inhibitor of HIV replication with a broad range of activity. In this study, the hepatic disposition of cosalane was investigated with a noncirculating isolated perfused rat liver technique. When 6 microM cosalane was infused into livers from untreated rats, the drug was highly extracted by the liver (only 2. 5% of influent cosalane concentration appeared in the effluent perfusate). Pretreatment of rats with various inducers of cytochrome P-450 before perfusion neither altered the effluent cosalane concentration nor resulted in the appearance of detectable metabolites in the effluent perfusate or liver homogenates. Hepatic uptake of cosalane was negligible when the drug was infused in the presence of BSA, and infusion of albumin after cosalane resulted in a significant displacement of the drug into the effluent perfusate. Furthermore, permeabilization of perfused livers with digitonin significantly diminished effluent cosalane concentration while enhancing cosalane uptake by the liver. Based on our data, it appears that a significant proportion of cosalane does not penetrate the hepatocyte membrane and may accumulate in the lipid bilayer of the cell membrane. This finding supports the proposed mechanism explaining the antiviral effect of cosalane which stipulates that this compound appears to imbed perpendicularly in the lipid bilayer of the cell membrane and the viral envelope. Also, cosalane does not seem to be metabolized by the liver as evidenced by the lack of detectable metabolites in the effluent perfusate, liver homogenates, and liver microsomal incubations.

Digitonin

10.1016/s0090-9556(24)11830-2

Cite

Citations (5)

Protein Binding of Bile Acids in Rat Liver Cytosol and Microsomes

Clinical Science (1980)

J. C. Ellis Barbara H. Moreland G.M. Murphy

Conference Abstract| September 01 1980 Protein Binding of Bile Acids in Rat Liver Cytosol and Microsomes Julia Ellis; Julia Ellis 1Gastroenterology Unit, Department of Medicine and the Biochemistry and Chemistry Department, Guy's Hospital Medical School, London Search for other works by this author on: This Site PubMed Google Scholar Barbara H. Moreland; Barbara H. Moreland 2Gastroenterology Unit, Department of Medicine and the Biochemistry and Chemistry Department, Guy's Hospital Medical School, London Search for other works by this author on: This Site PubMed Google Scholar G. M. Murphy G. M. Murphy 3Gastroenterology Unit, Department of Medicine and the Biochemistry and Chemistry Department, Guy's Hospital Medical School, London Search for other works by this author on: This Site PubMed Google Scholar Clin Sci (Lond) (1980) 59 (3): 24P. https://doi.org/10.1042/cs059024Pa Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation Julia Ellis, Barbara H. Moreland, G. M. Murphy; Protein Binding of Bile Acids in Rat Liver Cytosol and Microsomes. Clin Sci (Lond) 1 September 1980; 59 (3): 24P. doi: https://doi.org/10.1042/cs059024Pa Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu nav search search input Search input auto suggest search filter All ContentAll JournalsClinical Science Search Advanced Search This content is only available as a PDF. Article PDF first page preview Close Modal You do not currently have access to this content.

Icon

Title page

10.1042/cs059024pa

Cite

Citations (0)

Insulin inhibition of tyrosine transaminase degradation in the isolated, perfused rat liver.

PubMed (1968)

Per O. Seglen

Transaminase

Degradation

Tyrosine aminotransferase

Source