Investigation of tumour supressor protein p53 in renal cell carcinoma patients from Slovakia.
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Abstract:
Background. Investigation of p53 immunoreactivity in formalin-fixed paraffin-embedded tissues of normal renal tissue and renal cell carcinoma with respect to histopathologic subtype and nuclear grade of RCC. Methods. 42 tissue sections of RCC and 5 samples of normal renal tissue were stained for p53 expression using immunohistochemical assay. The results were analyzed in relation to nuclear grade and histopathologic subtype. Results. In total, p53 expression was found to be 4 to 5 times higher (30.8%) in other types of RCC than in the clearcell type of RCC (6.9%). Further, there was no statistically significant difference in p53 overexpression among the histopathologic subtypes (P>0.05, P=0.063). No association was found between the expression of p53 and nuclear grade (P>0.05, P=0.17). Interestingly, our study also showed weak cytoplasmic positivity in renal tubular epithelium. Conclusion. Our findings suggest that p53 might play an important role in tumour development or progression and it might be used as a new predictor and therapeutic target for RCC.Keywords:
P53 protein
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Introduction: Our aim was to evaluate the overexpression of p53 protein, product of mutated TP53 gene, in histologic sections of the kidneys with renal cell carcinoma (RCC) and its association with tumor grade and subtype. Materials and Methods: A total of 66 histologic sections of the kidneys of patients with the diagnosis of RCC were re-evaluated and tumor grade, tumor subtype, and p53 expression were determined. Results: Of the total 66 histologic sections with the diagnosis of RCC, 34 (51.5%), 27 (41%), and 5 (7.5%) were conventional, papillary, and chromophobe subtypes, respectively. Fifty-one (77.3%), 14 (21.2%), and 1 (1.5%) of tumors were grade 2, 3, and 4, respectively. Thirty (45.4%) sections were positive for p53 immunohistochemical staining. In 7 cases (20.6%) of the conventional tumors, p53 staining was positive, while 18 papillary (66.6%) and 5 chromophobe tumors (100%) had a positive staining for p53 (P < .001). Seventeen out of 51 grade 2 tumors (33.4%) and 12 out of 14 grade 3 tumors (85.7%) were positive for p53. The single case of grade 4 tumor was positive for p53 protein, too (P = .001). Conclusion: Increased expression of p53 protein is rather prevalent in RCC. This factor is associated with tumor grade and subtype. According to our findings, it is generally accompanied by nonconventional subtypes and higher tumor grades.
Chromophobe cell
P53 protein
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The aim of this study was to investigate the relationship of AEG-1 and p53 with the prognostic parameters of renal cell carcinoma (RCC). In this study, 50 paraffin blocks were histopathologically diagnosed at the Department of Pathology of the Medical Hospital of Duzce University, between 2005 and 2011. The cases consisted of 24 clear cell (CC) and 26 non-clear cell (NCC) RCC subtypes as follows: 24 (48%) clear cell RCC, 12 (24%) papillary RCC, 4 (8%) multilocular cystic RCC and 10 (20%) chromophobe RCC; none had sarcomatoid changes. By immunohistochemical analysis we investigated AEG-1 and p53 expression in carcinomas of the kidney, and by statistical analysis determined their relationship with clinicopathological parameters. Significant relationships were found between increasing tumor diameter and the increase of p53 (p = 0.028). In addition, p53 was significantly related to renal sinus invasion (p = 0.05) and Fuhrman grade (p = 0.026). There was a significant relationship between increased AEG-1 staining scores and CC and NCC carcinoma subtypes (p = 0.032), tumor capsule invasion (p = 0.01) and lymphovascular invasion (p = 0.015). There was also a significant correlation between tumor size and capsule and lymphovascular invasion (p = 0.02). We concluded that high AEG-1 and p53 expression correlates with the prognostic parameters in RCC patients, and in addition may be associated with tumor progression.
Chromophobe cell
Lymphovascular invasion
Tissue microarray
Renal sinus
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Objective
To observe the expression levels of Vimentin and Ki-67 in the occurrence and development of renal cell carcinoma (RCC) and explore the possible correlation between the expression of Vimentin/Ki-67 and clinical significance.
Methods
The clinical data of 133 patients who underwent surgical operation from January 2016 to June 2017 were reviewed. SP immunohistochemical staining was used to explore the expression levels of Vimentin and Ki-67 in different tissues. Correlation analysis was carried out between the expression results and the clinical stage, histological grade and tumor size.
Results
Both Vimentin and Ki-67 showed positive expression in renal cell carcinoma, but no expression was found in the adjacent normal renal tissue. Vimentin was mainly expressed in the cytoplasm of the cells while Ki-67 expressed in nucleus. There was significant difference between renal cell carcinoma and adjacent normal renal tissue (χ2=58.86, P 0.05; χ2=1.16, P>0.05), age(χ2=0.03, P>0.05; χ2=0.87, P>0.05) and location(χ2=0.00, P>0.05; χ2=0.00, P>0.05)of renal cell carcinoma patients. However, there were significant differences between different clinical stage (χ2=10.66, P<0.01; χ2=19.88, P<0.01), histological grade (χ2=13.79, P<0.01; χ2=21.03, P<0.01) and tumor size (χ2=19.52, P<0.01; χ2=16.10, P<0.01). There was positive correlation between Vimentin/Ki-67 (r=0.46, P<0.05).
Conclusions
Vimentin and Ki-67 are involved in the development of renal cell carcinoma, and their expression levels are related to the clinical stage, histological grade and tumor size. High expression levels of Vimentin and Ki-67 are presented in carcinoma tissues with high clinical stage, late histological grade and big tumor. Both of them may play a synergistic role in the progression of the carcinoma.
Key words:
Kidney neoplasms; Vimentin; Ki-67; Immunohistochemistry
Clinical Significance
Ki-67
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[purpose]To investigate the relationship among p53 and p21 expression,pathological grading and recurrence in human giant cell tumors of bone (GCT).Monoclonal antibodies against p53 and p21 proteins were used to measure expression of these proteins in the nuclei or cytoplasm of cells from sections of the giant cell tumor of bone. Among 52 cases,the positive expression rates of p53 and p21 were 26.9%(14/52) and 13.5%(7/52) respectively,which had no relationship with grading (P=0.699;P=0.658).The positive expression rates of p53 and p21 were 46.2% and 20.5% in recurrent cases,and 23.1% and 10.3% in cases without recurrence,respectively.Two cases had positive expression both in p53 and p21 protein.Both p53 and p21 overexpression closely related to recurrence of GCT (P=0.042).[Conclusion]The expression of p53 and p21 protein is related to tumor recurrence,but no relationship with pathological grade.
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P53 protein
Giant Cell Tumors
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OBJECTIVE To determine the ability of p63 reactivity to confirm the diagnosis of urothelial carcinomas (UCs) that present clinically as kidney masses and to determine the association of p63 expression with clinicopathologic features in UCs. STUDY DESIGN The study included 27 patients with renal pelvis UC and 42 patients with renal cell carcinoma (RCC). p63 Reactivity was examined immunohistochemically in tumor and nonneoplastic tissues. Correlation of p63 expression in renal malignancies (RCCs and UCs) was investigated and compared with clinicopathologic parameters. RESULTS p63 Expression was detected in 25 of 27 (92.6%) UC cases while none of the RCC cases were positive for p63. There was a correlation between p63 expression and tumor stage, grade and survival time (p < 0.0001, = 0.032, = 0.028, respectively). However, no correlation was found between p63 expression and tumor progression (p = 0.513). CONCLUSION p63 Should be considered as an additional biomarker to distinguish UC from RCC. Decreased p63 immunoreactivity was significantly associated with advanced tumor stage and grade. p63 Reactivity appears to be a useful prognostic factor in UC.
Renal pelvis
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Great attention is paid to renal cell carcinoma (RCC) due to the growth of its prevalence. This paper is dedicated to the matching of classic clinical and pathological prognostic factors to the character of the expression of proliferation marker Ki-67 in this kind of cancer. The proliferative activity was analyzed in 101 RCC cases by immunohistochemical assays using antibodies to Ki-67. In aggressive RCC and Fuhrman grade 3 to 4 tumors, Ki-67 level was found to be high more often. Besides, time to the appearance of remote metastases was significantly shorter, and total survival rate was significantly lower in RCC with high proliferative activity vs. low.
Ki-67
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ObjectiveTo study the expression of MMP9 and PCNA in renal cell carcinoma(RCC) and its relation to the RCC histological types,clinical stages,and prognosis,respectively.MethodsMMP9 and PCNA expression were immunohistochemically detected in 35 RCC cases and 12 cases of normal renal tissues adjacent to neoplastic lesions.ResultsThe positive rate of MMP9 was higher in tumor tissue than that in nonneoplastic tissue(P=0.020),and correlated with the stages,grades,and prognosis of the patients,there was no significant difference between clear RCC and granular cell carcinoma.The positive rate of PCNA in tumor tissue was higher than that in normal renal tissues adjacent to neoplastic lesions(P=0.008),and was related to the pathological grades and the prognosis of the patients.There exist no significant differences of the positive rate of PCNA between the pathological stages(H=5.746,P0.05) of RCC or the histological types(P=0.329).ConclusionMMP9 and PCNA could be used as predictors for supplemental diagnosis,evaluation of the malignancy and prognosis of RCC.
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Nephrology
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Purpose:To study the correlation between P16 protein and tumorgrade,stage,prognosis of renal cell carcinoma(RCC).Methods:P16 protein was investigated in 51 cases of RCC using immunohistochemical technique.Results:The results showed that the total positive rate of P16 was 52.9 %. The P16 positive rate in gradeⅠ,Ⅱand Ⅲ was 66.7 %, 71.4 % and 37.0 %,respectively and was 69.7 % and 22.2 % in clear cell carcinoma and granular cell carcinoma.There was a significant difference in survival rate between P16 positive and negative cases.Conclusions:The result suggested that P16 play an important role in suppressing the development of renal cell carcinoma and can be used as a predictor of malignance and prognosis.
Positive correlation
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Objective To investigate the expressions and significance of P21 and P27 proteins in ovarian carcinoma. Methods The expressions of P21 and P27 protein in normal ovarian tissue(10 cases), benign ovarian tumor (10 cases) and ovarian carcinoma (30 cases) were analyzed by immunohistochemistry technique. Results The positive rates of P21 and P27 in normal ovarian and benign ovarian tumor groups were higher than that in ovarian carcinoma (P=0.014,0.004). The expression of P21 and P27 in carcinoma was associated with clinical stage (P=0.024,0.008), but not correlated with pathological type, histologic grade, ascites or lymphatic metastasis (P=0.067-0.562). Positive correlation was found between P21 and P27 expression in the three tissues (P=0.039). Conclusion The abnormal expression of P21 and P27 may directly affect cell cycle progression and tumorigenesis.Protein examination,especially combined examination of P21 and P27,has positive effects on prognosis and treatment of the cancer.
Ovarian tumor
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