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    EFFECT OF WATER DEPTH ON GROWTH PERFORMANCE AND SURVIVAL RATE OF MIXED SEX NILE TILAPIA FINGERLINGS AND ADULTS
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    Abstract:
    Nile tilapia (O.niloticus) fingerlings and adult averaging (18 and 100 g in weight) respectively, were reared under 3 different depths ( 75,125 and 175cm) for 90 days to study the effect of water depth on the growth and the survival of overwintered fishes using twelve standing cages.Fish were fed on a commercial diet containing 26.58 % protein.The dissolved oxygen , temperature, pH, UIA-N and transparency of water and mortality were recorded for each cage every 15 days during the experimental period.Also, at the end of the experimental period , the fish were harvested , counted and the growth measurements ( weight and total length) were determined .The statistical analyses indicated that there were significant differences (≤0.05) in all parameters between all depths.Results showed that the final average total length, weight gain, condition factor and survival rate being the best at depth of 175 cm in both two sizes of tilapia during the cold season in Egypt.
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    Nile tilapia
    In response to DNA damage, p53 undergoes post-translational modifications (including acetylation) that are critical for its transcriptional activity. However, the mechanism by which p53 acetylation is regulated is still unclear. Here, we describe an essential role for HLA-B-associated transcript 3 (Bat3)/Scythe in controlling the acetylation of p53 required for DNA damage responses. Depletion of Bat3 from human and mouse cells markedly impairs p53-mediated transactivation of its target genes Puma and p21 . Although DNA damage-induced phosphorylation, stabilization, and nuclear accumulation of p53 are not significantly affected by Bat3 depletion, p53 acetylation is almost completely abolished. Bat3 forms a complex with p300, and an increased amount of Bat3 enhances the recruitment of p53 to p300 and facilitates subsequent p53 acetylation. In contrast, Bat3-depleted cells show reduced p53–p300 complex formation and decreased p53 acetylation. Furthermore, consistent with our in vitro findings, thymocytes from Bat3-deficient mice exhibit reduced induction of puma and p21, and are resistant to DNA damage-induced apoptosis in vivo. Our data indicate that Bat3 is a novel and essential regulator of p53-mediated responses to genotoxic stress, and that Bat3 controls DNA damage-induced acetylation of p53.
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    In the present study the effects of the seaweed Taonia atomaria supplementation in diets for Nile tilapia were investigated. A 12-week feeding trial was carried out using triplicate groups of Nile tilapia, Oreochromis niloticus with 1.1±0.04 g initial weight. Four experimental diets were formulated and marked as D1 (control), D2, D3, and D4. There were significant differences (P
    Nile tilapia
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    The present experiment was conducted to study the tolerance of Nile Tilapia to low temperature stress and its duration.Nile Tilapia was continuously stressed by artificial decreasing temperatures and the lethal time of Nile Tilapia under different low temperature stress was recorded.The results showed that the semi-lethal duration of Nile Tilapia was 12.25,17.00,25.00 and 31.50 h(P0.05) under low temperatures of 7,8,9 and 10℃,respectively.That is,the semi-lethal du-ration decreased with decreasing temperatures,however,the mortality of Nile Tilapia was higher at lower temperature stress.The regression equation between death duration and mortality of Nile Tilapia was y =0.0024x2.2453(R2 =0.9667) and y = 0.0013x2.0554(R2=0.9516) at 7 and 8℃,respectively.While it showed linear correlation at 9 and 10℃ temperatures,and the e-quation was y=0.0552x-0.6854(R2=0.9535) and y=0.0288x-0.2875(R2=0.9523),respectively.
    Nile tilapia
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    A 28-week rearing trial was conducted in twelve one cubic meter cages with (30.2 ± 3.2 g/fish) Nile tilapia, Oreochromis niloticus mono sex males fingerlings, to test the feasibility of rearing Nile tilapia mono sex in floating cages placed in the River Nile systems, to find out the optimum stocking density per m 3 that could give maximum yield of market-size fish, also, the weight and length of fish at stocking and to determine the physico-chemical characteristics of the water of El-Bagoria canals at El-Hamoul, Menoufiya governate. Fish were fed a least cost diet containing 30.2% crude protein and 4600 kcal /kg diet. The results revealed that Nile tilapia average weight 30.2 g/fish with 150 fish /m 3 as stocking density were adequate for rearing. These fish gave the best both of average weight gain of 117 g/fish and total production of 22.1 kg/m 3 . The artificial food supplied with a crude protein content of 30.2 % is adequate for rearing Nile tilapia in running water systems. The physico-chemical property of the water of El-Bagoria canals was found to be adequate for rearing of Nile tilapia in cages.
    Nile tilapia
    Nile delta
    Cage
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    HLA-B-associated transcript 3 (BAT3) was originally identified as one of the genes located within human major histocompatibility complex. It encodes a large proline-rich protein with unknown function. In this study, we found that a fragment of the BAT3 gene product interacts with a candidate tumor suppressor, DAN, in the yeast-based two-hybrid system. We cloned the full-length rat BAT3 cDNA from a fibroblast 3Y1 cDNA library. Our sequence analysis has demonstrated that rat BAT3 cDNA is 3617 nucleotides in length and encodes a full-length BAT3 (1098 amino acids) with an estimated molecular mass of 114,801 daltons, which displays an 87.4% identity with human BAT3. The deletion experiment revealed that the N-terminal region (amino acid residues 1-80) of DAN was required for the interaction with BAT3. Green fluorescent protein-tagged BAT3 was largely localized in the cytoplasm of COS cells. Northern hybridization showed that BAT3 mRNA was expressed in all the adult rat tissues examined but predominantly in testis. In addition, the level of BAT3 mRNA expression was more downregulated in some of the transformed cells, including v-mos- and v-Ha-ras-transformed 3Y1 cells, than in the parental cells.
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    SUMMARY A hitherto unrecorded virus having flexible rod‐shaped particles about 740–760 × 13 nm was isolated from Anthoxanthum odoratwn L. It was transmitted by sap inoculation, but not by several species of insect, seed or soil to 18 species of Gramineae including wheat, oats and barley. In susceptible species the virus normally produced a mosaic mottling of the leaves which was sometimes followed by a necrotic streaking or striping.
    Mosaic virus
    Fertilization of ultraviolet (UV) irradiated oocytes of Nile tilapia, Oreochromis niloticus (L.), with sperm from O, niloticus or blue tilapia, O, aureus (Steindachner), and subsequent suppression of the first cleavage of fertilized eggs successfully induced androgenesis in Nile and blue tilapia. The optimal doses of UV irradiation to denucleate a female genome of Nile tilapia prior to androgenesis ranged from 5940 to 6930 erg mm−2 for 54-63 s at a fixed intensity of 110 erg mm−2 s−1. Putative androgenetic fish were created from eggs which were irradiated at various times and several durations of heat-shock. Eggs which were treated for 5 min at 41.6 oC at 2 7.5 min after fertilization were the most successful at suppressing the first cleavage and producing viable androgenetic diploids in Nile or hybrid Nile X blue tilapia. The maximal survivals of putative androgenetic diploids in relation to the control were 1.60% and 0.90% in Nile and hybrid Nile X blue tilapia, respectively. The androgenetic offspring established exhibited active feeding and normal growth.
    Nile tilapia
    Nile blue
    This study aimed to compare the growth and survival of Nile tilapia (Oreochromis niloticus) fed with three different feed types: homemade feed (control), commercial feed, and earthworm-enriched feed. A total of 135 Nile tilapia with an average weight of 6.2 g and length of 6.5 cm were stocked in 9 hapas, with 15 fish per hapa. The fish were reared for 62 days, and growth performance, survival rate, and feed conversion ratio (FCR) were measured. The results showed that the final average weight and length of Nile tilapia fed with earthworm-enriched feed were higher than those fed with homemade and commercial feed. The highest mean weight gain (18.45 g) and mean length gain (4.7 cm) were observed in Nile tilapia fed with earthworm-enriched feed (T3R1). The FCR of Nile tilapia fed with earthworm-enriched feed (1.65) was lower than that of the other two groups. Moreover, the survival rate of Nile tilapia fed with earthworm-enriched feed was 100%, while the survival rate of Nile tilapia fed with homemade and commercial feed was 93.3% and 66.7%, respectively. In conclusion, earthworm-enriched feed showed superior performance in terms of growth and survival rate of Nile tilapia. This feed type can be considered as a potential alternative to the traditional homemade and commercial feed in the aquaculture industry. Further studies on the economic feasibility of using earthworm enriched feed for Nile tilapia are recommended.
    Nile tilapia
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