An Approach to Quantify Endomembrane Dynamics in Pollen Utilizing Bioactive Chemicals
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Abstract:
花粉试管和根头发的尖端生长经由快速的极的生长发生。这些很快伸长的房间为免职要求 tip-focusedendomembrane trafficking 并且蛋白质，膜，和房间墙材料再循环。大多数出版到日期的 image-baseddata 主观、非确定。这些的量、比较的描述符高度 dynamicprocesses 是主要挑战，但是是高度合乎需要的让基因、化学的 genomics 途径把 thisbiological 网络。处理这个问题，我们为使不安的小分子屏蔽了标记 forthe Golgi 像地岬的 monomeric G 蛋白质 RAB2 的本地化: 在转基因的烟草花粉表示的 GFP。自动化半的高产量的 imagingand 图象分析导致了改变了花粉试管开发和 endomembranetrafficking 的新奇混合物的鉴定。引起了错误本地化和 RAB2 的改变的运动的变化的度的六混合物:标记 GFP 的 endomembranebodies 被用来产生确定的图象数据的一个训练集合泡动力学。每身体的区域，速度，笔直，和紧张用揭示每混合物引起的量的差别 inthe 显型的自动化半的图象分析工具被确定。一个分数然后被给启用在结果表明的 compounds.Our 之间的量的比较的每混合物那图象分析能习惯于份量上作为策略的部分由 bioactive 化学药品，变化，或另外的使不安的代理人评估动态 subcellular endomembrane phenotypesinduced 到份量上把 theendomembrane network.Key 词：Keywords:
Endomembrane system
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Endomembrane system
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Endomembrane system
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ABSTRACT GFP technology is revolutionising our current understanding of plant cell biology as its application permits the study of cellular events in vivo. This has shed new light not only on the morphology of cells but also on the dynamics of organellar systems and their interrelationships within the cytosol. The aim of this review is to illustrate how GFP technology can be applied to the study of plant cells and then to detail current results obtained in the study of the endomembrane system in plants based around current work in our laboratories.
Endomembrane system
Plant cell
Dynamics
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