Effect of Dietary Folic Acid Supplementation on Growth Performance and Hepatic Protein Metabolism in Early-Weaned Intrauterine Growth Retardation Piglets
3
Citation
0
Reference
5
Related Paper
Citation Trend
Keywords:
Growth retardation
Folic acid supplementation
Cite
Abstract Trihexanoin is a short-chain triglyceride (SCT). Many studies have reported that SCTs play important roles in the maintenance of intestinal epithelial structure and function. The present work was to investigate the effects of trihexanoin on growth performance, carbohydrate and fat metabolism, as well as intestinal morphology and function in weaned piglets. Twenty weaned piglets (21 ± 2 d) were randomly allocated to one of two treatment groups: the control group (basal diet supplemented with 0.5% soya oil); the TH group (basal diet supplemented with 0.5% trihexanoin). Dietary trihexanoin supplementation significantly reduced diarrhea rate (P < 0.05); increased the concentrations of LDL, HDL and total protein, decreased cholesterol concentrations (CHOL) and glutamyl transpeptidase (GGT) activity in plasma (P < 0.05); increased villus height, surface area, and the ratio of villus height to crypt depth (P < 0.05); altered the mRNA levels and abundances of proteins related to glycogen and fat metabolism (gene LIPE, LPL, PPARG, ACACA, FASN, SLC27A2, INSR, PCK1 and ASS1), mucosal barrier function (protein claudin-1, and occludin), antioxidant capacity (protein HSP70 and gene Nrf2, NOX2 and GSTO2) and water transport capacity (protein AQP3 and gene AQP8 and AQP10) (P < 0.05); altered the gene abundance of intestinal bacteria (Enterobacteriaceae, Enterococcus, Clostridium, Lactobacillus and Bifidobacterium) (P < 0.05). In conclusion, dietary supplementation of trihexanoin improved the intestinal function and health of weaned piglets by regulating nutrient metabolism, improving intestinal function of mucosal barrier, transport, absorption and antioxidant, and altering the community of microbiota.
Barrier function
Bifidobacterium bifidum
Occludin
Intestinal Permeability
Cite
Citations (0)
Abstract The objective of this study was to determine the effects of a Saccharomyces cerevisiae yeast as a substitute to AGP on intestinal health and performance of piglets. A total of 192 piglets weaned at 28 d of age were randomly assigned into one of four treatment groups with 8 pens/ treatment and 6 pigs/pen. Piglets were supplemented with either a regular nursery diet with two phase feeding (Phase 1: d1-d14 and Phase 2: d15-d28) (CON); CON diet supplemented with 75mg/kg Chlortetracycline plus ZnO 2000 mg/kg in phase 1 and 160 mg/kg in phase 2 (AGP); Con diet supplemented with 2kg/ton live yeast in both phases (LY); Con diet plus live yeast 1kg/ton in both phases plus ZnO 2000 mg/kg in phase 1 and 160 mg/kg in phase 2 (LY-ZnO). During Phase 1, LY and LY-ZnO group had significantly higher ADG (p< 0.01). In Phase 2 only the LY group has a significantly higher ADG (P=0.02). The LY and LY-ZnO group had also better feed efficiency (P< 0.05) during phase 1. On the other hand, the CON group had significantly higher (P< 0.01) diarrhea score. Test on antioxidant status has not shown any significant difference between the groups for most parameters measured; however, the concentration of Malondialdehyde (MDA, a marker for oxidative stress) was significantly decreased (P< 0.01) in the LY and LY-ZnO group on day 28. Furthermore, superoxide dismutase (SOD, the most powerful antioxidant) was significantly increased (P< 0.05) in these two treatment groups at different time points. Live yeast and Live yeast plus ZnO treatment has also significantly increased (P< 0.01) concentration of IgA and IgG in the serum of piglets on d7 and d14. In conclusion, supplementation of nursery piglet diets with either live yeast or live yeast plus ZnO improved zootechnical performance of animals by improving gut health through reduction of oxidative stress and enhancement of immune response, suggesting that yeast probiotics could be a suitable alternative to substitute AGP.
Malondialdehyde
Chlortetracycline
Cite
Citations (1)
Fatty Acid Metabolism
Carbohydrate Metabolism
Cite
Citations (16)
Cite
Citations (0)
To investigate the effects of dietary supplementation with folic acid on growth performance, hepatic protein metabolism and serum biochemical indices of early-weaned intrauterine growth retardation (IUGR) piglets, 24 male (Duroc×(Landrace×Yorkshire)) weaned (14-d-old) IUGR piglets were randomly divided into 3 treatments with 8 replicates of 1 piglet per replicate. The piglets in each treatment were fed basal diet supplementation with either 0 (control), 5 and 10 mg kg−1folic acid. The trial lasted for 21 d. Dietary folic acid supplementation reduced average daily feed intake (ADFI) (P<0.05). In addition, the average daily gain (ADG) in 10 mg kg−1 folic acid group was significantly decreased (P<0.01) and the ratio of feed:gain (F/G) increased slightly (P>0.05). Serum folic acid concentration increased (P<0.01) with increasing folic acid inclusion, however, serum homocysteine concentration decreased significantly (P>0.01). Enhanced serum urine nitrogen (SUN) and diminished serum total protein (TP) as well as liver TP content were observed in 10 mg kg−1 folic acid group (P<0.05). Furthermore, the relative mRNA expressions of insulin-like growth factor 1 (IGF-1) and mammalian target of rapamycin (m-TOR) in liver were respectively tended to reduce (P=0.06) and significantly downregulated (P<0.05) in 10 mg kg−1 group, in compared with 5 mg kg−1 group. However, when compared with control group, folic acid supplementation had no significant effect on the mRNA abundance of IGF-1 and m-TOR. The results indicated that supplementation with 10 mg kg−1 folic acid impaired growth performance and hepatic protein metabolism of early-weaned IUGR piglets while 5 mg kg−1 folic acid enriched diet exerted limited positive effects.
Folic acid supplementation
Cite
Citations (13)