Detection of human papilloma virus (HPV) in parafin block of cervical cancer patients using multiplex polymerase chain reaction (PCR) and reverse line blot methods
Muhammad Husni CangaraAgus Priyo WibowoUpik Anderiani MiskadBerti NelwanRina MasadahSyahrul RaufSyarifuddin Wahid
0
Citation
8
Reference
10
Related Paper
Abstract:
The aim of this study is to detect human papilloma virus (HPV) especially types 16 and 18 through DNA expression from paraffin block preparations of cervical cancer and pre-cancer. This study was conducted at the Anatomical Pathology Laboratory and Molecular biology Hasanuddin University Medical Research Center (HUMRC) Laboratory. The samples that used were came from cervical specimens which had been processed into paraffin blocks and had been diagnosed as Cervical Intra-epithelial Neoplasia (CIN) and Cervical carcinoma. HPV DNA was extracted from paraffin blocks using DNA extraction reagents from FFPE (Qiagen). DNA extraction products were then propagated by the polymerase chain reaction (PCR) method. HPV genotyping is done by a reverse line blot method using Ampliquality HPV-Type Express (AB Analitica). Totally 67 cervical samples from paraffin blocks preparations were diagnosed in Squamous Cell Carcinoma (SCC) and Cervical Intra-epithelial Neoplasia (CIN). The large number of cancer patients was more than 40 years old (42 patients/67.7%), with most of which are in the periods 41–50 years old (25 patients/40.3%). The most common cancer type was Non Keratinizing Squamous Cell Carcinoma (NK-SCC) with 50 (74.6%) samples. The most infectious strains are HPV strains 16, which were found in 53 (79.1%) samples both in single infection and co-infection with other HPV strains. Three of four HPV strains found were 16, 18 and 52 strains that belonging to High Risk strain of HPV, while strain 67 was included in the low risk group. There was also co-infection of 2 different HPV strains which involved 16 & 67 HPV Co-infection and 52 & 67 HPV Co-infection. Cervical cancer patients were more commonly found in women with more than 30 years old, which the most common type was Non keratinizing squamous cell carcinoma (NK-SCC). HPV infection is strongly associated with cervical cancer. HPV 16 infection was found in many cervical cancer lesions both as a single infection and co-infection with other types of low risk HPV.Keywords:
Multiplex
A case of laryngeal squamous papilloma in the early stages of development showed histological features suggestive of virus infection. Five years later positive evidence of HPV-6 infection was obtained at a time when the lesion had developed into a squamous cell carcinoma. It is concluded that this case represents a complete example of the virus to papilloma to carcinoma sequence, and as far as is known, is the first reported case of its kind in the larynx.
Cite
Citations (64)
Association (psychology)
Cite
Citations (0)
Inverted Papilloma
Complete response
Cite
Citations (10)
Objective To investigate the clinical effects of circumcision on infection of high-risk human papilloma virus(HPV) in adult males.Methods A total of 276 adult males with circumcision were collected and analyzed in our hospital.Another 276 phimosis without circumcision were recruited in the control group.The high-risk HPV was detected by HPV-DNA genotyping chip.Results 157 cases were infected by high-risk HPV in 552 phimosis,and the infection rate was 28.4%.Before circumcision,80 cases were infected by high-risk HPV with an infection rate of 29.0% in treatment group,and 77 cases were infected by high-risk HPV with an infection rate of 27.9% in control group.There was no significant difference between the two groups(P0.05).After circumcision,the infection rate of high-risk HPV was significantly decreased from 29.0% to 11.9% in treatment group(P0.05).However,the infection rate of high-risk HPV was 29.3% in control group and no significant difference was found cmparing with two years ago(P0.05).Conclusion Circumcision can effectively reduce high-risk HPV infection,and which was worthy to promote the clinical application.
Infection rate
Male Circumcision
Infection Risk
Risk of infection
Cite
Citations (0)
Squamous papilloma is the most common benign oral epithelial lesion, and it is well known to be associated with human papilloma virus 6 and 11. Here, we report a case of squamous papilloma associated with human papilloma viruses (HPV)-32 in a 4-year-old boy who presented with a verrucous lesion on the lower lip. HPV-32 is often associated with a rare benign condition focal epithelial hyperplasia (FEH). A limited number of lesions and the absence of characteristic histology ruled out FEH in our patient. To the best of our knowledge, the association of oral squamous papilloma with HPV-32 is hitherto unreported.
Papillomaviridae
Cite
Citations (4)
RT-PCR-based examination of papilloma samples obtained from patients with relapsing papillomatosis of the larynx showed an incidence rate of human papilloma virus (HPV) amounting to 89%. The viral load level of the studied samples, when measured by concurrent RT-PCR HPV, differed by more than 130 times. It made, in the untreated patient, 1.2 x 10(9) hormonal equivalents/ml, i.e. 13-fold higher versus the patient who received pathogenetic therapy. Thus, the approach in question provides for a possibility to monitor the activity of papilloma viral infection and to evaluate the efficiency of different variations of pathogenetic therapy because the "classic" variant of PCR-detection is not informative in the discussed case.
Papillomatosis
Cite
Citations (0)
Objective To understand human papilloma virus(HPV)infection situation of women in the east area of Guangdong.Methods HPV of 569 women in the east area of Guangdong were examined by gene chip technique.Results Of 569 cases investigated,275 cases were HPV positive(48.3%),183 cases were single HPV type infection and 67 cases were double HPV types infection and 25 cases were multiple HPV types infection respectively.In 275 HPV positivecases,there were 147 cases of HPV-16,31 cases of HPV-18,83 cases of HPV-11 and 96 cases of HPV-6 respectively.Conclusions In the east area of Guangdong,HPV infection situation of women was severe,it should be very important to control HPV infection and treat cervical erosion effectively.The gene chip technique is a sensitive and specific method for examining HPV.
Cite
Citations (0)
Sinonasal papilloma is a relatively rare disease. However, it is prevalent enough for every otorhinolaryngologist to encounter it several times throughout one's medical practice. The aim of this study was to identify the presence of Human Papilloma Virus in sinonasal specimens of patients with sinonasal papilloma. A cross sectional analytical study was performed on fresh tissue samples from 36 patients with sinonasal papilloma. Samples were studied by polymerase chain reaction for of Human Papilloma Virus detection. In conclusion, the majority of patients were of Human Papilloma Virus negative and there was no statistically significant difference in presence of squamous cell carcinoma in of Human Papilloma Virus positive and negative patients. Thus, further studies are needed to assess other potential factors that may influence the development of sinonasal papilloma.
Inverted Papilloma
Cite
Citations (2)
Papillomatosis
Recurrent Respiratory Papillomatosis
Cite
Citations (0)
Objective:To study the relationship between infection of human papilloma virus and juvenile pharynlaryngeal papilloma. Methods:Polymerase chain reaction (PCR) and dot blot hybridization were used to detect HpV 6、11、16、18、33 DNA in 35 samples of pharynlaryngeal papilloma and 10 samples of .vocol nodule. Results:The positive rate of HPV in pharynlaryngeal laryngeed papilloma was 91.4% (30/35) . The positive rate of HPV 6 and HPV 1 1 were 54.2% (19/35) and 25.7% (9/35). The positive rate of multiple types of HPV 6+11 was 11.4% (4/35).The positive rate of HPV 16、18、33 were not significant. The positive rate of HPV in vocol nodule was not significant .Conclusion:It is suggested that infection with HPV,especially with HPV 6 is closely associated with the development of juvenile pharynlaryngeal larynged papilloma in Wenzhou area. [FK(WB80011。5]
Papillomatosis
Dot blot
Southern blot
Cite
Citations (0)