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    Activation of the NRF2 Pathway in Non-adherent Mammosphere Culture System
    추계총회 및 학술대회 (2013)
    Ryoo In-geunKwak Mi-Kyoung
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    Cancer Cells and Metastasis
    [Differences in expression of Notch1, E-cadherin, vascular endothelial growth factor A and C, and chemokine receptor 4 gene in breast suspension mammosphere cells and normal adherent cells].
    PubMed (2013)
    Zhaohui WangChuanwei LiYu AiLianGuangfu YuYinghui Zhao
    Notch1是参与调控细胞分化的重要分子,其表达异常与许多肿瘤的转移密切相关.在乳腺癌发展过程中,Notch1分子可能通过增强乳腺癌干细胞迁徙活性促进其转移,如果证实这一机制,对于研制靶向于乳腺癌干细胞Notch1分子的药物,阻断其转移有重要意义。
    VE-cadherin
    Citations (0)
    Nrf2 activation drive macrophages polarization and cancer cell epithelial-mesenchymal transition during interaction
    Cell Communication and Signaling (2018)
    Rui FengYuji MorineTetsuya IkemotoSatoru ImuraShuichi Iwahashi
    The M2 phenotype of tumor-associated macrophages (TAM) inhibits the anti-tumor inflammation, increases angiogenesis and promotes tumor progression. The transcription factor Nuclear Factor (erythroid-derived 2)-Like 2 (Nrf2) not only modulates the angiogenesis but also plays the anti-inflammatory role through inhibiting pro-inflammatory cytokines expression; however, the role of Nrf2 in the cancer cell and macrophages interaction is not clear.Hepatocellular carcinoma cells (Hep G2 and Huh 7) and pancreatic cancer cells (SUIT2 and Panc-1) were co-cultured with monocytes cells (THP-1) or peripheral blood monocytes derived macrophages, then the phenotype changes of macrophages and epithelial-mesenchymal transition of cancer cells were detected. Also, the role of Nrf2 in cancer cells and macrophages interaction were investigated.In this study, we found that cancer cells could induce an M2-like macrophage characterized by up-regulation of CD163 and Arg1, and down-regulation of IL-1b and IL-6 through Nrf2 activation. Also, Nrf2 activation of macrophages promoted VEGF expression. The Nrf2 activation of macrophages correlated with the reactive oxygen species induced by cancer cells derived lactate. Cancer cells educated macrophages could activate Nrf2 of the cancer cells, in turn, to increase cancer cells epithelial-mesenchymal transition (EMT) through paracrine VEGF. These findings suggested that Nrf2 played the important role in the cancer cells and macrophages interaction.Macrophage Nrf2 activation by cancer cell-derived lactate skews macrophages polarization towards an M2-like phenotype and educated macrophages activate Nrf2 of the cancer cells to promote EMT of cancer cells. This study provides a new understanding of the role of Nrf2 in the cancer cell and TAM interaction and suggests a potential therapeutic target.
    Macrophage polarization
    CD163
    Citations (148)