Novel use of culturomics to identify the microbiota in hospital sink drains with and without persistent VIM-positive Pseudomonas aeruginosa
Jannette PirzadianSusan P. HarteveldShanice N. RamduttWillem J. B. van WamelCorné H. W. KlaassenMartine De VosJuliëtte A. Severin
19
Citation
31
Reference
10
Related Paper
Citation Trend
Abstract:
Abstract In hospitals, Verona Integron-encoded Metallo-beta-lactamase (VIM)-positive Pseudomonas aeruginosa may colonize sink drains, and from there, be transmitted to patients. These hidden reservoirs are difficult to eradicate since P. aeruginosa forms biofilms that resist disinfection. However, little is known on the composition of these biofilms. Therefore, culturomics was used for the first time to investigate the viable microbiota in four hospital sink drain samples with longstanding VIM-positive P. aeruginosa drain reservoirs (inhabited by high-risk clone, sequence type ST111), and four drain samples where VIM-positive P. aeruginosa was not present. Microbial load and composition varied between samples, yielding between 471–18,904 distinct colonies and 8–20 genera. In two VIM-positive drain samples, P. aeruginosa was the most abundantly-isolated microorganism, and found in combination with other Gram-negative bacteria, Citrobacter , Enterobacter , or Stenotrophomonas . P. aeruginosa was in low abundance in the other two VIM-positive samples, and found with Gram-positive cocci ( Enterococcus and Staphylococcus ) or Sphingomonas . In VIM-negative drain samples, high abundances of Gram-negative non-fermenting bacteria, including Acinetobacter , non-aeruginosa Pseudomonas spp., Acidovorax , Chryseobacterium , Flavobacterium , and Sphingobium , as well as Candida , were cultured. Although additional experiments are needed to draw more firm conclusions on which microorganisms enable or inhibit VIM-positive P. aeruginosa persistence, our data provide unique insights into the microbial compositions of sink drain inlets.Keywords:
Stenotrophomonas
Stenotrophomonas maltophilia
Enterococcus faecalis
Stenotrophomonas maltophilia
Stenotrophomonas
Cite
Citations (3)
Stenotrophomonas maltophilia
Stenotrophomonas
Marine invertebrates
Cite
Citations (36)
Background: Stenotrophomonas species are multi-resistant bacteria with ability to cause opportunistic infections.
Objective: We isolated 45 Stenotrophomonas species from soil, sewage and the clinic with the aim of investigating their susceptibility to commonly used antimicrobial agents.
Methodology: The identities of isolates were confirmed with 16S rRNA gene sequence and MALDI-TOF analysis. Anti-mi- crobial resistance, biofilm production and clonal diversity were also evaluated. The minimum inhibitory concentration technique as described by Clinical & Laboratory Standards Institute: CLSI Guidelines (CLSI) was employed for the evaluation of isolate susceptibility to antibiotics.
Result: Forty-five Stenotrophomonas species which include 36 environmental strains and 9 clinical strains of S. maltophilia were considered in this study. 32 (88.9 %) environmental strains were identified to be S. maltophilia, 2 (5.6 %) were Stenotrophomonas nitritireducens, and 2 (5.6 %) cluster as Stenotrophomonas spp. Stenotrophomonas isolates were resistant to at least six of the antibiotics tested, including Trimethoprim/Sulfamethoxazole (SXT).
Conclusion: Environmental isolates from this study were resistant to SXT which is commonly used for the treatment of S. maltophilia infections. This informs the need for good public hygiene as the environment could be a reservoir of multi-resistant bacteria. It also buttresses the importance of surveillance study in the management of bacterial resistance.
Keywords: Stenotrophomonas; environmental; biochemical characterization; clonal diversity; anti-microbial susceptibility.
Stenotrophomonas maltophilia
Stenotrophomonas
Sulfamethoxazole
Trimethoprim
Cite
Citations (13)
Stenotrophomonas maltophilia
Stenotrophomonas
Gram-negative bacterial infections
Burkholderia cepacia complex
Cite
Citations (0)
Stenotrophomonas sepilia strain SM16975 (= JCM 32102; = KCTC 62052) is a new species isolated from the blood culture of a hospitalized patient. The biochemical characterization, phenotypic criteria, phylogenomic reconstruction, and genomic analysis were carried out to differentiate it from its phylogenetic neighbours, establishing novel species status in the genus Stenotrophomonas and within Stenotrophomonas maltophilia complex (Smc).
Stenotrophomonas maltophilia
Stenotrophomonas
Blood Culture
Strain (injury)
Cite
Citations (11)
During the analysis of a collection of
Stenotrophomonas maltophilia
Stenotrophomonas
Cite
Citations (2)
목적 : Stenotrophomonas maltophilia 각막염의 원인과 임상양상, 치료결과 및 예후에 대해 알아보고자 하였다. 대상과 방법 : 각막도말 및 배양검사 상 Stenotrophomonas maltophilia 각막염으로 진단 받고 3개월 이상 추적관찰이 가능하였던 환자를 대상으로 과거력, 시력, 발생부위 및 크기, 전방축농 및 치료에 대한 반응 등을 조사하였다. 결과 : Stenotrophomonas maltophilia 각막염 10명 10안 중 남자는 7명, 여자는 3명이었으며, 평균 연령은 62.9±11.3세였다. 2안(20.0%)에서만 각막외상력이 있었고 7안(70.0%)에서 복합감염이었는데 그 중 3안에서 Pseudomonas종, 2안에서 Fusarium종이었다. 6안에서 각막중심부에 발생하였고, 병변의 크기는 평균 28.5±19.6 mm2였으며, 9안에서 전방축농이 있었다. 7안(70.0%)에서는 수술적 치료를 시행하였다. 초진시 시력은 9안(90.0%)에서 0.02미만이었고, 치료 후 3안(30.0%)에서 시력 호전이 있었으나, 7안(70.0%)에서는 안전수동 이하로 시력이 불량하였다. 결론 : Stenotrophomonas maltophilia 각막염은 주로 안구표면의 방어기전이 손상된 환자들에서 복합감염으로 발생하여 불량한 예후를 보인다.
Stenotrophomonas maltophilia
Stenotrophomonas
Gram-negative bacterial infections
Cite
Citations (0)
This study reports the presence of the blaNDM-1 gene in an isolate of Stenotrophomonas maltophilia obtained from a Brazilian soil, inside an IncA/C plasmid with ~ 45 Kb. To the best of our knowledge, this is the second report in the world and the first in Brazil of NDM-producing bacterium isolated from soil.
Stenotrophomonas maltophilia
Stenotrophomonas
Gram-negative bacterial infections
Cite
Citations (17)
To assess the influence of Stenotrophomonas on plants, the interaction of 16 Stenotrophomonas strains from clinical and environmental sources with strawberry plant seedlings was analysed.In vitro, all Stenotrophomonas strains influenced plant growth when applied to seedlings. Whereas most of the Stenotrophomonas strains promoted root growth and hair development, a statistically significantly negative influence on the length of stem was found. Although strains from a clinical origin also showed statistically significant effects on plants, this was generally lower when compared with environmental strains. For three selected strains, a strong dose-dependent effect was observed for all parameters. In vitro, a correlation was found between plant growth promotion and production of a plant growth hormone, indole-3-acetic acid (IAA). Xanthomonas campestris, a phylogenetically very closely related species to Stenotrophomonas, was used as a phytopathogenic control. It too confirmed the reduction of plant growth in this in vitro system.Independent of their origin, Stenotrophomonas strains can produce IAA in vitro and subsequently, influence plant growth. The effect of Stenotrophomonas presence on plants was dose-dependent.The dose-dependent effect of Stenotrophomonas, a bacterium of both biotechnological and medical interest, is of great interest for biocontrol applications of plant-associated strains. This paper is the first report that clearly demonstrates the phytopathogenic capacity of Stenotrophomonas.
Stenotrophomonas
Stenotrophomonas maltophilia
Cite
Citations (86)
Objective To evaluate the curative effect of antibiotic for ventilator-associated pneumonia(VAP)infected with stenotrophomonas maltophilia(SM)and provide the evidences for treat- ment.Methods 72 patients were involved in this study and divided into two groups:non-treated group and treated group with antibiotic.To compare the prognosis of patients in two groups.Results There were 34 patients in the non-treated group:5 were cured,14 showed obvious improvement and 15 were ineffec- five;there were 38 patients in antibiotic treated group:7 were cured,19 showed obvious improvement and 12 were ineffective.There were no significant differences in results between the two groups(χ~2=1.20,P0.05).Conclusion There was no evidence that antibiotics are effective for ventilator-associated pneu- monia infected with stenotrophomonas maltophilia.
Stenotrophomonas maltophilia
Stenotrophomonas
Ventilator-associated Pneumonia
Cite
Citations (0)