Spontaneous hyaline glomerulopathy in a young Slc:ICR mouse
0
Citation
17
Reference
10
Related Paper
Abstract:
Hyaline glomerulopathy is a type of glomerular lesion that occurs in aging mice. Spontaneous hyaline glomerulopathy is rare in young mice. Here, we report spontaneous hyaline glomerulopathy in a young adult (15-week-old) ICR mouse. Necropsy revealed discoloration and roughness of the kidney surface. Microscopically, diffuse glomerular lesions were prominent. Amorphous, eosinophilic materials were deposited globally in the glomeruli. The mesangial region was expanded; however, the mesangial cells showed no proliferation. Thickening of the Bowman's capsule with proliferation of parietal epithelial cells was observed. Glomerular deposits were strongly positive for anti-IgM, anti-IgG, and periodic acid-Schiff stain and were stained red by Masson's trichrome stain. The deposits were negative for anti C3 and stained negatively with Congo red stain. Periodic acid methenamine silver and electron microscopy revealed glomerular deposits limited to intraglomerular capillaries. Based on the histological features, we diagnosed this lesion as hyaline glomerulopathy. This case could improve our understanding of spontaneous lesions in toxicological and pharmacological studies.Keywords:
Hyaline
Glomerulopathy
Periodic acid–Schiff stain
Stain
Trichrome
The periodic acid—Schiff stain is one of the most valuable methods now available for the demonstration of fungi in tissue.1In our hands this method has proved superior to the routine hematoxylin and eosin stain for this purpose, and on more than a few occasions it has permitted us to demonstrate the presence of pathogenic fungi that were invisible in routine sections. Because of the failure of this staining method to win widespread acceptance, we are prompted to cite additional instances in which a correct diagnosis was not made until the periodic acid—Schiff stain was used. We are convinced that wider use of this method in the study of bacteriologically negative tuberculomas and nonspecific pulmonary granulomas will result in an increase in frequency of identification of the deep mycoses. The development and the various applications of the periodic acid—Schiff stain have been described in detail by Gomori.2The
Stain
Periodic acid–Schiff stain
Periodic acid
Eosin
Cite
Citations (16)
Little is known about the relationship between splenic arteriolar hyaline and cause of death. The purpose of this retrospective study was to evaluate the clinicopathological significance of splenic arteriolar hyaline in autopsy cases and estimate the applicability of hyaline for diagnosing the cause and rapidity of death. Archival data and histological slides from 82 cases were reviewed retrospectively. One section of each spleen was evaluated microscopically. The tinctorial pattern of splenic arteriolar hyaline was examined with Heidenhain's Azan trichrome stain, and the relationships between this pattern and age, cause of death, and rapidity of death were investigated. Fifty-four cases demonstrated hyaline change, with 3 different tinctorial patterns: red, blue, and a combination of red and blue. The 3 patterns coexisted in various proportions in each tissue section. Frequency of the blue pattern increased with age (P < 0.01) and was unrelated to cause of death. By contrast, the red pattern was unrelated to age and appeared with different frequency according to cause of death. The red pattern appeared with significantly higher frequency in the circulatory disease group and the drowning and asphyxia group (both P < 0.01). Moreover, the presence of the red pattern had high specificity for the detection of rapidly fatal cases. The combination of the 2 colors was excluded from clinicopathological analyses due to its admixed nature. Estimation of splenic arteriolar hyaline with Heidenhain's Azan trichrome stain is useful for assessment of the cause and rapidity of death. The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1132441651796836
Hyaline
Trichrome
Trichrome stain
Masson's trichrome stain
Forensic Pathology
Stain
Cite
Citations (7)
This is a report of an unusual mesodermal tumor present on the neck of a 65-year-old male Negro. The lesion was 1.5 cm high, skin colored, solid, cylindrically shaped and firmly bound to the underlying structures. On histochemical analysis there were three outstanding features namely (a) hyaline material, (b) an intense net of reticulum fibers, and (c) xanthoma cells. The hyaline material, which represented the bulk of the tumor, was strongly PAS positive, diastase resistant, and stained blue with Mallory's trichrome, thus suggesting the presence of collagen with an unusual increase in bound-carbohydrates. We are proposing to designate this tumor as a hyaline reticuloxanthoma.
Hyaline
Trichrome
Cite
Citations (0)
OBJECTIVE: To investigate whether the presence of mucin defines a combined hepatocellular‐cholangiocarcinoma or merely a variant of usual hepatocellular carcinoma (HCC). METHODS: From 1979‐92, 124 cases of usual HCC were studied at Niigata University Hospital, Niigata City, Japan, and several affiliated hospitals. Histological diagnoses were determined according to World Health Organization (WHO) criteria. Hematoxylin and eosin stain, periodic acid‐Schiff (PAS) stain, PAS stain after diastase digestion (D‐PAS) and silver stain tests were performed. Cases containing D‐PAS‐positive substances were also stained by Alcian blue (AB) stain, high iron diamine (HID) stain and concanavaline A paradox‐3 type (ConA3) stain. The classification of mucin was determined by AB, HID and ConA3 stains. RESULTS: Mucin was recognized in the area of HCC by mucin stains in 25 of 124 cases. Two forms of mucin existence were classified: extracellular and intracellular. Mucins were classified by histochemical stains into three types: sulfomucin, sialomucin and neutral mucin. CONCLUSIONS: According to the WHO histological classification of primary carcinoma of the liver, mucin existence is characteristic of intrahepatic cholangiocarcinoma. But if mucin exists in morphologically usual HCC, it is better to diagnose it as a variant of HCC (with mucin) rather than as a combined hepatocellular‐cholangiocarcinoma.
Stain
Periodic acid–Schiff stain
Cite
Citations (0)
Hyaline glomerulopathy with tubulo-fibrillary deposits was observed in two young female ddY mice. One of the mice showed gross systemic edema and bilateral enlargement and pale color of the kidneys, whereas no significant gross findings were noted in the other mouse. Microscopically, a large number of the glomeruli in both mice were enlarged because of diffuse and global deposition of amorphous eosinophilic materials. The deposits were negatively stained with Congo red and positively stained with IgG, IgM, IgA, C3, and periodic acid–Schiff. Electron microscopic examination revealed microtubular and fibrillary deposits with diameters of 80–100 and 9–16 nm, respectively, in the subendothelial space of the glomeruli. These features are histopathologically similar to immunotactoid glomerulopathy or fibrillary glomerulonephritis according to the classification of human glomerular lesions. Understanding of these characteristics of hyaline glomerulopathy in ddY mice is essential when evaluating pharmacological, pharmacokinetic, and toxicological studies using this mouse strain.
Hyaline
Glomerulopathy
Cite
Citations (4)
Tissues embedded in plastic are sometimes stained with a special stain for fungi. Sections of plastic-embedded tissue were stained using both the periodic acid-Schiff (PAS) reaction and Grocott’s methenamine silver (GMS) method. The periodic acid-Schiff reaction is a more rapid and less complicated stain, which stained evenly. Grocott’s methenamine silver method stained inconsistently, occasionally caused sections to detach from the slides, and is a longer and more complicated stain. Thus, the periodic acid-Schiff reaction is recommended for demonstrating fungi in plasticembedded tissue.
Stain
Periodic acid–Schiff stain
Periodic acid
Cite
Citations (2)
Introduction: Oral submucous fibrosis (OSMF) is graded according to various histological factors which include the epithelial changes and the connective tissue changes. These features though could be identified in routine hematoxylin and eosin (H and E) staining; they could be better appreciated in special stains. This pilot study is an attempt to identify a single special stain that can act as an adjunct to H and E stain to help grade this potentially malignant disease. Aims and Objectives: To assess if special stains can improvise on differentiating the various histological changes seen in OSMF and to accordingly grade OSMF cases. Materials and Methods: Formalin-fixed paraffin-embedded tissue sections of OSMF-10 cases of each grade (n = 30). Three special stains: Van-Gieson, Mallory's trichrome and Masson trichrome. Statistical Analysis: The results obtained were tabulated and statistically analyzed using Chi-square test. Observations and Results: The thickness and degree of keratinization were best detected in Mallory's stain (100%) and were statistically significant; the subepithelial changes were better detected using special stains, especially Mallory's stain (100%). The changes in collagen fibers were better visualized in all three special stains but were not statistically significant. The changes in blood vessels were better detected in Van-Gieson's and Mallory's stain; the obtained results were statistically significant. The degree of fibrosis between muscle bundles could be detected in all the three special stains, but when compared the results were not statistically significant. The questionable areas of muscle degeneration, especially in deeper connective tissue were better detected in Mallory's (43%) and Masson's stain (43%) as compared to Van-Gieson stain (14%) and the results obtained were statistically significant. The inflammatory cells and dysplastic features are better visualized in routine H and E stains. Conclusion: Pathogenesis of OSMF is related to fibro-elastic and muscle degenerative changes in the connective tissue followed by secondary changes in epithelium. Routine H and E, stains all the connective tissue components in various shades of pink, use of special stains bestows contrast between different components of connective tissue, thus improvising grading of OSMF. Mallory's stain can be used as a single adjunct to H and E stain as both pattern of keratinization in the epithelium and changes in the superficial and deeper connective tissue could be ascertained.
Oral submucous fibrosis
Adjunct
Grading (engineering)
Stain
Cite
Citations (14)
Histologic evaluation of renal biopsies includes multiple ancillary stains, including Periodic acid-Schiff's (PAS) and Masson's trichrome (Trichrome). Herein we report an innovative double-stain, derived from two standard stains (PAS and Trichrome). This novel stain not only has advantages of both ancestor stains, but became more distinguishable and colorful, when basement membranes stain dark-violet, whereas the interstitial collagen remains blue. This allows the pathologist immediate estimation of the amount of collagen, tubular atrophy and the degree of interstitial fibrosis in one section. Using computer-based analysis, we confirmed that our innovative double-stain highlights interstitial collagen better than Trichrome stain alone. We strongly recommend renal pathologists to try this innovative stain in their practice.
Trichrome stain
Stain
Trichrome
Masson's trichrome stain
Periodic acid–Schiff stain
Cite
Citations (11)
Basement membrane (BM) is a thick sheet of extracellular matrix molecules, upon which epithelial cells attach. Various immunohistochemical studies in the past have been carried out but these advanced staining techniques are expensive and not feasible in routine laboratories. Although hematoxylin and eosin (H-E) is very popular among pathologists for looking at biopsies, the method has some limitations. This is where special stains come handy.The aim of the present study was to demonstrate and compare the efficacy of H-E, periodic acid Schiff (PAS) and fluorescent periodic acid-acriflavine staining techniques for the basement membrane and to establish a histochemical stain which could be cost effective, less time consuming, and unambiguous for observation of the basement membrane zone.A total number of 40 paraffin-embedded tissue sections of known basement membrane containing tissues including 10 - Normal oral mucosa (NOM) and 30 - oral lichen planus (OLP) were considered in the study. Four-micron-thick sections of each block were cut and stained with H-E stain, PAS and fluorescent periodic acid-acriflavine stain. Sections were evaluated by three oral pathologists independently for continuity, contrast and pattern.Though all the three stains showed favorable features at different levels, acriflavine stain was better than the other stains in demonstrating BM continuity, contrast and also the pattern followed by PAS stain. Acriflavine stain was the better in demonstrating a fibrillar pattern of a BM. Acriflavine stains a BM distinctly and is less time consuming and easy to carry out using readily available dyes as compared to other stains.The continuity and contrast along with the homogenous pattern and the afibrillar pattern of the BM was better demonstrated by acriflavine followed by the PAS stain.
Acriflavine
Stain
Periodic acid–Schiff stain
Eosin
Cite
Citations (19)
Periodic acid–Schiff stain
Stain
Periodic acid
Sudan Black B
Cite
Citations (6)