Gene polymorphisms (rs324957, rs324981) in NPSR1 are associated with increased risk of primary insomnia
Yuping XieYuan ZhaoLiya ZhouLijun ZhaoJinfeng WangWei MaXiaoyan SuPeilin HuiBin GuoYü LiuJie FanShangli ZhangH. J. YangWenjuan ChenJing Wang
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Neuropeptide S and neuropeptide S receptor (NPSR1) are associated with sleep regulation. Herein, the possible contribution of 6 polymorphisms in NPSR1 on the chromosome to primary insomnia (PI) and objective sleep phenotypes was investigated.The study included 157 patients with PI and 133 age- and sex-matched controls. All subjects were investigated by polysomnography for 3 consecutive nights. The genotyping of 6 polymorphisms was carried out by polymerase chain reaction-restriction fragment length polymorphism method.A significant difference was detected for rs324957 and rs324981 between PI and controls. The PI patients had a higher frequency of AA than controls in rs324957 (P = .02) and rs324981 (P = .04). However, for other single nucleotide polymorphisms (rs323922, rs324377, rs324396, and rs324987), no significant differences were observed between PI patients and controls. There were 2 different allelic combinations that were associated with PI susceptibility (CATGTC, GCCAAT) and its risk factor. A significant difference in sleep latency was observed among 3 genotype carriers of NPSR1 gene polymorphism rs324957 in PI group (P = .04), with carriers of the A/A genotype having the longest sleep latency (mean ± SD: 114.80 ± 58.27), followed by the A/G genotype (112.77 ± 46.54) and the G/G genotype (92.12 ± 42.72).This study provided the evidence that the NPSR1 gene polymorphisms (rs324957, rs324981) might be susceptibility loci for PI. Further studies are needed to explore the role of NPSR1 gene polymorphisms in molecular mechanisms of PI in a larger sample size.Helicobacter pylori(Hp) infection can cause gastritis and peptic ulcer.The genotyping can show the essence of life.Different genotyping is correlated with different diseases and different genotyping correlated with different resistances.This article observed the Hp genotyping.There were four aspects to discuss:The correlation on different genotyping with the disease;different genotyping resistant;the different genotyping mucosal damage intensity;intervention link of traditional Chinese medicine.A brief summary of the nearly seven years of Hp genotyping research provides a new method to discover new drugs for the treatment of Hp with traditional Chinese medicine.
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Objective:Classified HBV DNA of AsC of children and their parents. Methods:Using a genotyping method of based on the restriction fragment length polymorphism (RFLP) of amplified segment of the HBV S region.Results: Among the AsC of children living in Guangzhou,genotype B is 62.7%,genotype C is 33.3%,genotype B and C is 3.3%,only 1.7% could not be classified.Conclusion:The method for genotyping is simple and conveniat,the prevalent HBV strain in Guangzhou is genotype B and genotype C.
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The aim of the study was to determine AAT genotype by the simple DNA-based method in a group of ten Croatian families. AAT genotype was determined by PCR-RFLP in samples taken from each member of the ten families (mother, father and child/children). In the group of parents, five normal genotypes, Pi MM and fifteen Pi MZ genotypes, were detected. In the group of children, particular genotypes followed the mode of inheritance. There were eight Pi MZ, and five Pi ZZ genotypes. PCR-RFLP was found to be the method of choice for AAT genotyping. Determination of AAT genotype in family studies enables the risk of deficient allele inheritance to be followed-up and assessed. Early diagnosis of a deficient AAT genotype contributes to the success of currently widely available AAT replacement therapy.
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Abstract Background: HIV-1 drug resistance genotyping is critical to the monitoring of antiretroviral treatment. Data on HIV-1 genotyping success rates of different laboratory specimen types from multiple sources is still scarce. Methods: In this cross-sectional study, we determined the laboratory genotyping success rates (GSR) and assessed the correlates of genotyping failure of 6837 unpaired dried blood spot (DBS) and plasma specimens from multiple studies/sources. Specimens from multiple studies in a resource-constrained setting were analysed in our laboratory between 2016 and 2019. Results: We noted an overall GSR of 65.7% and specific overall GSR for DBS and plasma of 49.8% and 85.9% respectively. The correlates of genotyping failure were viral load (VL) <10,000 copies/mL (aOR 0.3 95% CI: 0.24-0.38; p<0.0001), lack of viral load testing prior to genotyping (OR 0.85 95% CI: 0.77-0.94; p=0.002), use of DBS specimens (aOR 0.10 95% CI: 0.08-0.14; p<0.0001) and specimens from routine clinical diagnosis (aOR 1.4 95% CI: 1.10-1.75; p=0.005). Conclusions: We report rapidly decreasing HIV-1 genotyping success rates between 2016 and 2019 with increased use of DBS specimens for genotyping and note decreasing median viral loads over the years. We recommend improvement in DBS handling, viral load testing prior to genotyping and development of more sensitive assays to genotype specimens with low or undetectable viral load, especially in this era where virological suppression rates are rising due to increased antiretroviral therapy roll-out.
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Objective To establish a diagnostic method for ABO genotyping and to provide a simple and reliable technique for safe transfusion and identification of complicated blood group. Methods ABO genotyping method was developed by using multiplex-PCR-RFLP and PCR-SSP techniques. Results We developed a set of methods, which were stable and accurate for ABO genotyping. This diagnostic system were excellent for ABO genotyping after implementation. Conclusions We have established an reliable ABO genotyping diagnostic system.
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Abstract Background HIV-1 drug resistance genotyping is critical to the monitoring of antiretroviral treatment. Data on HIV-1 genotyping success rates of different laboratory specimen types from multiple sources is still scarce. Methods In this cross-sectional study, we determined the laboratory genotyping success rates (GSR) and assessed the correlates of genotyping failure of 6837 unpaired dried blood spot (DBS) and plasma specimens. Specimens from multiple studies in a resource-constrained setting were analysed in our laboratory between 2016 and 2019. Results We noted an overall GSR of 65.7% and specific overall GSR for DBS and plasma of 49.8% and 85.9% respectively. The correlates of genotyping failure were viral load (VL) < 10,000 copies/mL (aOR 0.3 95% CI: 0.24–0.38; p < 0.0001), lack of viral load testing prior to genotyping (OR 0.85 95% CI: 0.77–0.94; p = 0.002), use of DBS specimens (aOR 0.10 95% CI: 0.08–0.14; p < 0.0001) and specimens from routine clinical diagnosis (aOR 1.4 95% CI: 1.10–1.75; p = 0.005). Conclusions We report rapidly decreasing HIV-1 genotyping success rates between 2016 and 2019 with increased use of DBS specimens for genotyping and note decreasing median viral loads over the years. We recommend improvement in DBS handling, pre-genotyping viral load testing to screen samples to enhance genotyping success and the development of more sensitive assays with well-designed primers to genotype specimens with low or undetectable viral load, especially in this era where virological suppression rates are rising due to increased antiretroviral therapy roll-out.
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Objective Classified HBV DNA of AsC of children living in Guangzhou. Methods Using a genotyping method based on the restriction fragment length polymorphism (RFLP) of amplified segment of the HBV S region. Results Among the AsC of children living in Guangzhou ,genotype B is 62.7%,genotype C is 33.3% genotype B and C is 3.3%,only 1.7%couldn not be classified . Conclusions The method for genotyping is simple and convenient,The prevalent HBV strain of children in Guangzhou is genotype B and genotype C.
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Objective Using PCR-RDB to establish a new method for HBV genotyping, and to survey the distribution of HBV genotypes in the Foshan area. Methods Biotin-labeled primers for amplification of HBV region X (nt1550-1789) were used to amplify extracted HBV DNA. HBV was genotyped by hybridization of the PCR products with immobilized specific probes (genotype A to F) on C membrane. Color development was achieved by adding POD and TMB. A judgment was made according to color reactions. The reliability of this new method was verified by gene sequencing. 300 samples of HBV DNA-positive sera from the Foshan area were genotyped using this assay. Results Of the 300 sera genotyped by PCR-RBD, 147 (49.0%) cases were genotype B, 136 (45.3%) were genotype C, 1 (0.3%) genotype D, and 12 (4.0%) were mixtures of genotype B and C, and 4 (1.3%) were mixtures of genotype C and D. No genotype A, E or F were found. The results of PCR-RDB genotyping were consistent with the results obtained with sequence analysis. Conclusion This newly established HBV genotyping system proved to be sensitive, specific, precise and economic, and should be suitable for clinical practice and epidemic study. The results of HBV genotyping show that genotype B and C are the predominant genotypes in the Foshan area.
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