Study on the quality standard of Samusake Oil.
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Abstract The purpose of this research is to compare the protein profile of herbals egg and non herbal egg using High Performance Liquid Chromatography (HPLC). This research used HPLC Shimadzu 6.1 with column C18, flow rate mobile phase 1 ml/min, wavelenght detector UV Vis 220 nm and temperature 50 o C. Mobile fase that used in this research was 10 and 60 % acetonitril (CH 3 CN) in water containing 0,05% triflouroacetic acid. Supplementation of herb containing bioactive antioxidant compounds affect the formation of egg yolk protein containing immunoglobulin. In this study, herbal eggs and non-herbal eggs were seen from their protein profile using the high performance liquid chromatography (HPLC) method and then analyzed using descriptive qualitative analysis. The result show that herbal egg yolk sample has a dominant protein with a molecular weight of 50.41 kDa. Herbal egg yolk protein appears at a retention time (RT) of 56.87 minutes, an area of 3303488 units of area, and the peak height of the graph / peak at 50,974 µAU. Meanwhile, non-herbal egg yolk sample has a dominant protein with a molecular weight of 49.94 kDa. This protein appears at a retention time of 1.307 minutes, an area of 149445550 units of area, and the peak height of the graph / peak at 402.6026 µAU. The results showed that the the peak of HPLC indicated an antioxidants were bound to the bioactive protein fractions of egg yolk. It could be concluded that bioactive herbal bound to egg yolk igY, but the bioactive compounds have not been identified yet.
Yolk
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Background: Organic acids (malic, citric, tartaric, oxalic, acetic, formic, isovaleric, ascorbic acids) make up a large group of biologically active substances and play an important role in plant and human metabolism. They are found in large quantities in the fruits of Rosaceae family medicinal plants that included in State Pharmacopoeia of the Russian Federation. Standardization of crude herbal drugs containing organic acids by modern physicochemical methods is a high-priority task. Materials and Methods: The determination of total organic acids amount was carried out in aqueous extracts from different fruits of Rosaceae family plants by galvanostatic coulometry and potentiometry methods. Galvanostatic coulometry was performed with the help of the “Expert-006” coulometer with a current of 5 mA (integrated pH meter). Iodine as an electrogenerated titrant was used for ascorbic acid determination; electro generation of hydroxide ions was carried out for determination of total organic acids amount. A potentiometer “Aquilon pH-410” with attached glass and silver chloride electrodes was used for potentiometric determination of total organic acids amount. Individual organic acids have been determined by reverse-phase high-performance liquid chromatography with ultra-violet detection (RP-HPLCUV) method. The following conditions were established: Gilson HPLC system, Alltech OA- 1000 Organic Acids (6.5×300 mm, 9 μm) chromatography column, a gradient elution mode, component A of the mobile phase is 98% (0.1% phosphoric acid, 10 mM KH2PO4, solution in water) with 2% acetonitrile, component B is acetonitrile, the eluent feed rate is 1 ml/min. Results: Modern physicochemical methods for the analysis of biologically active substances, organic acids, for quality control of crude herbal drugs and medicinal herbal preparations, are developed and discussed. The optimal conditions for the qualitative and quantitative organic acid analysis are selected and described taking into account modern pharmacopoeial requirements. Conclusion: Galvanostatic coulometry and potentiometry methods, as well as RP-HPLC-UV, can be successfully used in the quality control of crude herbal drugs and medicinal herbal preparations, specifically fruits of Rosaceae family plants. Development and validation of analytical methods for monitoring the content of this BAS group is an important research area in the pharmacopoeial standardization of crude herbal drugs.
Coulometry
Daminozide
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The assay was aimed to establish the quality standard of anti-virus Chinese herbal compound extract,so as to supply scientific evidence for the revolution of dosage form and industrialized production,we used TLC method and HPLC method to detect it qualitatively and quantitatively.The results showed that TLC could detect chlorogenic acid and HPLC could detect chlorogenic acid and glycyrrhizic acid.The established chromatographic method could be used in qualitative detection and preliminary quantitative detection exactly and laid the foundation for further study.
Chlorogenic Acid
Quality standard
Quantitative Analysis
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Pill
Quality standard
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Accelerated solvent extraction
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