Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in Rice
Yi WenYunxia FangPeng HuYiqing TanYueying WangLinlin HouXuemei DengHao WuLixin ZhuLi ZhuGuang ChenDali ZengLongbiao GuoGuangheng ZhangZhenyu GaoGuojun DongDeyong RenLan ShenQiang ZhangDawei XueQian QianJiang Hu
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Abstract:
Leaf shape is an important agronomic trait for constructing an ideal plant type in rice, and high-density genetic map is facilitative in improving accuracy and efficiency for quantitative trait loci (QTL) analysis of leaf trait. In this study, a high-density genetic map contained 10,760 specific length amplified fragment sequencing (SLAF) markers was established based on 149 recombinant inbred lines (RILs) derived from the cross between Rekuangeng (RKG) and Taizhong1 (TN1), which exhibited 1,613.59 cM map distance with an average interval of 0.17 cM. A total of 24 QTLs were detected and explained the phenotypic variance ranged from 9% to 33.8% related to the leaf morphology across two areas. Among them, one uncloned major QTL qTLLW1 (qTLL1 and qTLLW1) involved in regulating leaf length and leaf width with max 33.8% and 22.5% phenotypic variance respectively was located on chromosome 1, and another major locus qTLW4 affecting leaf width accounted for max 25.3% phenotypic variance was mapped on chromosome 4. Fine mapping and qRT-PCR expression analysis indicated that qTLW4 may be allelic to NAL1 (Narrow leaf 1) gene.Keywords:
Inbred strain
Genetic Analysis
Trait
Genetic Analysis
Positional cloning
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Inbred line 1507(dark green fruit skin) and inbred line 1508(white fruit skin) were used as the experiment materials for genetic analysis and gene mapping of white fruit skin in cucumber in this study.Genetic analysis showed that a single recessive nuclear gene,w,dominates the white fruit skin trait in cucumber.Bulked segregate analysis(BSA) and simple sequence repeat(SSR) technologies were employed to map w gene in F2 population.w was mapped to a linkage group with 14 SSR markers,corresponding to chromosome 3 of cucumber.The flanking markers SSR23517 and SSR23141 were linked to the w gene with genetic distances of 4.9 and 1.9 cM,respectively.The physical distance between SSR23517 and SSR23141 was 1 150 kb based on the whole genome sequence of cucumber,and there are 500 candidate genes in this region.These results will benefit fine-mapping of w gene and marker-assisted selection(MAS) in fruit skin color cucumber breeding program.
Cucumis
Candidate gene
White (mutation)
Genetic linkage
Genetic Analysis
Bulked segregant analysis
Genetic distance
Inbred strain
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Bulked segregant analysis
Cucumis
Melon
Genetic linkage
Genetic Analysis
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The RAPD analysis of Henan wild mouse and other four inbred strains of mouse were shown in this study.A total of 304 polymorphic fragments were achieved from 380 amplified fragments using 36 polymorphic primers which were selected from 120 random primers.Several specific bands possessed by wild mouse reflected that the RAPD polymorphisms exist among the four inbred strains of mouse and wild mice.The results of statistical analysis of RAPD bands showed that the genetic similarity between wild mice and PWK is 0.00924,which indicated that wild mice had the closest relationship to PWK.However,the genetic similarity between wild mice and DDK is 0.01130,which showed the wild mice were clearly apart from DDK.These results indicated that there were genetic similarities and variations between wild mouse and inbred strains of mouse.Thus,as a molecular marker,RAPD can be used to detect the variations and distinguish the mouse strains.
Inbred strain
Genetic Analysis
Genetic similarity
Laboratory mouse
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Family-based QTL mapping
Positional cloning
Inbred strain
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Primer (cosmetics)
Bulked segregant analysis
Rust (programming language)
Genetic Analysis
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Genetic mapping refers to the determination of distance and order of genes/QTL on the chromosome. The conventional linkage analysis has been used to this purpose leading to publication of first genetic map of 6 sexlinked genes on a fruit fly chromosome. In plants, genetic linkage maps have been developed for major gene controlling the qualitative traits in several crops. However, the possibility of locating the QTL controlling the quantitative traits has also been investigated earlier using morphological traits as genetic markers. However, the advent of molecular markers has sped up the development of dense molecular maps and QTL analysis in crop plants. This led to the identification of QTL for desirable traits of agronomic importance. In this chapter, we discussed the basic principles of molecular genetic mapping and QTL analysis in plants.
Family-based QTL mapping
Genetic linkage
Linkage (software)
Genetic Analysis
Genetic linkage map
Molecular marker
Candidate gene
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Wheat line 7K65 was resistant to Blumeria graminis f.sp.tritici Race E09.Genetic analysis showed that the resistance was controlled by a single dominant gene,named Ml7K65.After screening SSR,EST-STS markers,we found 7 marker loci were linked to the powdery mildew resistance gene Ml7K65.The genetic map of gene Ml7k65 indicates the order of these marker loci is Xcfa2086-XEST83-XEST89-Xksum193-Xmag633-Xcfd50-Ml7K65.The genetic distances of each interval are 5.4,2.8,0.4,2.4,0.4,2.0 cM,respectively.Xcfd50 is the closest marker,the genetic distance is 2.0 cM,while Xcfa2086 is the most distant maker with the genetic distance of 13.4 cM.Using Chinese Spring nullisomic-tetrasomic lines,we physically located the markers linked to Ml7K65gene on wheat chromosome 2A.
Blumeria graminis
Genetic distance
Genetic Analysis
Common wheat
Molecular marker
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The genetic analyses were conducted on attenuation characteristics of leaf stay-green after flowering in several maize inbred lines by using an additive-dominance-maternal genetic model.The results indicated that the time that plant beginning to senescence,the maximum reducing velocity of leaf stay-green,the time of the maximum reducing velocity were mainly controlled by genotype-environment interaction in different maize inbred lines,the performance of attenuation characteristics of leaf stay-green after flowering in several maize inbred lines were obviously difference.The difference of genetic effects of attenuation characteristics of leaf stay-green for different maize inbred lines could been discovered by an additive-dominance-maternal genetic model with genotype-environment interactions and corresponding statistical analysis approaches,screening the maize inbred lines with more great genetic effects for improving leaf stay-green.
Inbred strain
Dominance (genetics)
Genetic Analysis
Interaction
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Primer (cosmetics)
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Rust (programming language)
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