Studies of arctic Chlamydomonas sp. KNM0029C for biodiesel production

A sequential statistical optimization was applied to the components of Tris-acetate-phosphate (TAP) medium, to enhance the cell density and lipid production of Arctic microalga Chlamydomonas sp. KNM0029C. The evaluation was preformed through an elimination process and Plackett-Burman design to select for significant factors, contributing toward cell growth. NH₄Cl, MgSO₄·7H₂O, potassium phosphate, and trace elements were established as the effective components and used as variable factors in Box-Behnken design test, - a response surface methodology (RSM). The final optimized concentrations were 0.54, 0.16, 0.04, and 0.08 g/L of NH₄Cl, MgSO₄·7H₂O, potassium phosphate, and trace elements, respectively. Overall, 9.9% enhanced lipid production was achieved by using optimized TAP medium at 4°C. The results of the present study could potentially contribute toward large-scale lipid production at low temperatures.

Plackett–Burman design

Potassium phosphate

Box–Behnken design

Chlamydomonas

10.7841/ksbbj.2019.34.3.173

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