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    [Technical evaluation and principle analysis of simulative habitat cultivation of Dendrobium nobile].
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    Abstract:
    The technique of "simulative habitat cultivation" is to preserve the quality of traditional Chinese medicine by simulating the original habitat and site environment of wild Chinese medicine resources. Dendrobium nobile is the most representative variety of traditional Chinese medicine which reflects the coordinated development of medicinal material production and ecological environment. In this paper, the main technical points of the simulated cultivation model of D. nobile were summarized as follows: rapid propagation of seedling tissue technology to ensure the genetic stability of provenance; line card+fermented cow manure+live moss method to improve the survival rate; epiphytic stone cultivation to improve the quality of medicinal materials; and the integration of mycorrhizal fungi to improve the quality stability of medicinal materials. On the basis of summarizing the ecological benefits, economical and social benefits generated by the application of the technology, the paper systematically analyzes the principle of the technology for the cultivation of D. nobile to promote the excellent quality, the light, gas, heat and fertilizer resources of the undergrowth niche are in line with the wild site environment of D. nobile. The rich and complex soil microbial community in the forest laid the foundation for the species diversity needed for the growth of D. nobile.The stress effect on the growth of D. nobile resulted in the accumulation of secondary metabolites. The symbiotic relationship between the symbiotic fungi such as bryophytes and D. nobile promotes the synthesis of plant secondary metabolites. The high quality D. nobile was produced efficiently by improving and optimizing the cultivation techniques.
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    Biofertilizer
    Abstract A fundamental problem in ecology, regardless of habitat or system, is understanding the relationship between habitats and assemblage of organisms. It is commonly accepted that differences in composition and surrounding landscape of habitats affect the diversity of assemblages, although there is not much empirical evidence because of difficulties of manipulating structure in many habitats. These relationships were examined experimentally, using habitats of artificial turfs that are colonized by diverse assemblages of gastropods. Each habitat was made of nine sub‐habitats, which were sampled individually to allow tests of hypotheses about the effect of type of habitat and the influence of other adjacent sub‐habitats on the colonizing assemblage. Turf habitats were deployed for 8 weeks on a rocky shore after which they were collected and the colonizing assemblages of gastropods sampled. Independently of the types of turfs combined to form different habitats, there were more species where there was more than one type of component in a habitat (i.e. structural diversity). The type of habitat (i.e. structural identity) itself had little or no influence on the colonizing assemblage. The number of species colonizing short‐sparse and short‐dense turfs was influenced by which type of habitat was adjacent. Thus, when units of one type (e.g. short‐sparse turf) were added to a patch of habitat of long‐sparse turfs, the number of species in short‐sparse turfs was greater than in patches of the same type. This also increased total number of taxa in the whole patch of habitat. These results show how diversity of gastropods colonizing heterogeneous patches of habitat is influenced not only by the number of types of sub‐habitats, but also by interactions with surrounding sub‐habitats. These findings reiterate the importance of investigating the role of structure of habitats and of their surrounding landscapes across different systems, irrespective of their size or associated assemblages of organisms.
    Assemblage (archaeology)
    Phytophagous insects are astonishingly diverse, and their diversification is frequently shown to involve host shifting and host specialization. Habitat-associated differentiation may also occur for insects exploiting a single host in multiple habitats, although this is much less frequently reported. We tested for host- and habitat-associated differentiation in the holarctic and purportedly generalist leaf miner fly Nemorimyza posticata (Meigen) (Diptera: Agromyzidae), which is recorded from many host plants in the Asteraceae and from many different habitats. We collected larvae of N. posticata feeding on 11 host plant species in three habitat types in eastern Canada. Our analyses of sequence data for three fragments of two mitochondrial genes (COI and 12S) show substantial and complex genetic structure within N. posticata. The deepest split within N. posticata had over 5% sequence divergence, and we identified seven major clades with >2% sequence divergence. There was evidence for host-associated differentiation among these seven clades, but also within each; and even though host association explains most of the genetic diversity, there was evidence of habitat-associated differentiation. While N. posticata has been considered a polyphagous species, our data indicate that it is actually a complex of more host-specific lineages – a pattern increasingly understood to be common among phytophagous insects.
    Agromyzidae
    Lycaenidae
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