Coexisting overexpression of STOML1 and STOML2 proteins may be associated with pathology of oral squamous cell carcinoma
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Objective Three different staining techniques were used to visualize S180 ascites smear morphology.The results were compared to find a best and simple staining technique showing most clear cytological characteristics.Method S180 ascites smear was stained by H.E.staining,Pap staining and fast staining,and the cell morphology was observed and compared under microscope.Results Among the 3 staining methods,the fast staining was most simple to operate and revealed cellular structures best.There was a best contrast among cytoplasm,nucleus and nucleolus in the smears stained by the fast staining,and the cell structures can be distinguished most clearly.Conclusion The fast staining is better than HE staining and Pap staining in S180 Ascites smear observation.
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Subcellular localization is crucial to the study of virus and diseases. Specifically, research on protein subcellular localization can help identify clues between virus and host cells that can aid in the design of targeted drugs. Research on RNA subcellular localization is significant for human diseases (such as Alzheimer's disease, colon cancer, etc.). To date, only reviews addressing subcellular localization of proteins have been published, which are outdated for reference, and reviews of RNA subcellular localization are not comprehensive. Therefore, we collated (the most up-to-date) literature on protein and RNA subcellular localization to help researchers understand changes in the field of protein and RNA subcellular localization. Extensive and complete methods for constructing subcellular localization models have also been summarized, which can help readers understand the changes in application of biotechnology and computer science in subcellular localization research and explore how to use biological data to construct improved subcellular localization models. This paper is the first review to cover both protein subcellular localization and RNA subcellular localization. We urge researchers from biology and computational biology to jointly pay attention to transformation patterns, interrelationships, differences, and causality of protein subcellular localization and RNA subcellular localization. Subcellular localization is crucial to the study of virus and diseases. Specifically, research on protein subcellular localization can help identify clues between virus and host cells that can aid in the design of targeted drugs. Research on RNA subcellular localization is significant for human diseases (such as Alzheimer's disease, colon cancer, etc.). To date, only reviews addressing subcellular localization of proteins have been published, which are outdated for reference, and reviews of RNA subcellular localization are not comprehensive. Therefore, we collated (the most up-to-date) literature on protein and RNA subcellular localization to help researchers understand changes in the field of protein and RNA subcellular localization. Extensive and complete methods for constructing subcellular localization models have also been summarized, which can help readers understand the changes in application of biotechnology and computer science in subcellular localization research and explore how to use biological data to construct improved subcellular localization models. This paper is the first review to cover both protein subcellular localization and RNA subcellular localization. We urge researchers from biology and computational biology to jointly pay attention to transformation patterns, interrelationships, differences, and causality of protein subcellular localization and RNA subcellular localization.
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Objective To evaluate a kind of modified reticulocyte(Ret) staining solution.Methods The observation test of the modified Ret staining solution was performed under the microscope,counted the staining time,analyzed the effective life and tested its accuracy and precision.Results The modified Ret staining samples had a lot of characters,such as not easily fade,few residues,clear visual field and bright background,etc.Compared with the routine staining solution-brilliant cresyl blue,the staining time was short(5 min) and the effective life was long(30 months).The accuracy of the modified staining solution was consistent with that of the brilliant cresyl blue(P 0.05),but the precision of the modified staining solution was better than that of the brilliant cresyl blue.Conclusion The modified Ret staining solution is an excellent staining.It is worthy to be spreaded in practice.
Cresyl violet
Positive staining
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Background: There are various secreted proteins affecting the prognosis of oral squamous cell carcinoma (OSCC) and one of them is Angiopoietin-2(Ang-2) which is thought to have an essential role in the development and progression of the tumor. Aim of the study: This study was conducted to determine the expression of (Ang-2) in (OSCC) to assess its correlations with clinicopathological parameters of the tumor. Material and Methods: 36 formalin- fixed, paraffin- embedded tissue blocks histologically diagnosed as OSCC were examined for Ang-2 immunohistochemical expression semi quantitively. Results: The expression of Ang-2 was significantly associated with histopathological grade (P value=0.023), while there is no significant association with the clinical parameters analyzed in OSCC patients. Conclusion: A significant association between Ang-2 expression and histopathological grade of OSCC may predict its biological behavior.
Angiopoietin 2
Angiopoietin
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Abstract This unit describes protocols for detecting proteins in SDS‐polyacrylamide gels. It describes methods for Coomassie blue and silver staining, as well as the fluorescent stains SYPRO orange and red. Staining with Coomassie blue is easier and more rapid; however, silver staining methods are considerably more sensitive and can thus be used to detect smaller amounts of protein. Alternative rapid staining procedures are provided for each method. Fluorescent staining is a popular alternative to the traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining.
Coomassie Brilliant Blue
Silver stain
Fluorescent staining
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Objective: To compare three kinds of staining methods for proteins in SDS PAGE. Methods: The proteins in SDS PAGE were stained with coomassie brilliant blue staining, copper staining and silver staining. Then the proteins were restained after coomassie brilliant blue staining or copper staining. Results:The silver staining was the most sensitive, the sensitivity of the copper staining was the former between that of silver staining and the coomassie brilliant blue staining. It took only five minutes to stain proteins with copper staining. After copper staining, the proteins could be restained by silver staining or coomassie brilliant blue staining. Conclusions: Copper staining could be the first choice for rapid analysis of proteins in SDS PAGE. [
Coomassie Brilliant Blue
Silver stain
Stain
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Objective:To compare Lyon′s PAS standardized staining and traditional PAS staining.Methods:The sections of the same tissue were stained with the above two methods simultaneously.The results and replicability of the two methods were observed.Results:The posititive products and replicability of Lyon′s PAS staining were stronger than that of traditional PAS staining in the same tissue-sections.Coclusion:Lyon′s PAS standardized staining method is better than the traditional PAS staining method.
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Objective: To compare two staining methods.Methods: Two staining methods were used to stain tissue sec-tion and cell smear respectively,the preparation methods and the staining effects of two staining methods were evaluated.Results: The modified staining buffer has less sediment,no oxidized membrane,don't need filtration,has shorter staining time and better staining effects.Conclusion:The modified staining method is better than the old method and can result in better staining effects.
Stain
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The Subcellular Localizations of Non-structural Proteins of Southern Rice Black-streaked Dwarf Virus
Abstact Studies on the cellular localization patterns of a viral protein can provide clues to understand its mode of action. The six genes encoding non-structural proteins of southern rice black-streaked dwarf virus(SRBSDV) were cloned and expressed in Nicotiana benthamiana cells,respectively. Their subcellular localizations were observed by confocal fluorescent microscopy. The results showed that Pns52 and Pns91 were localized in the cytoplasm and formed large aggregates; Pns6,Pns71,Pns72 and Pns92 were localized in the cell cytoplasm and/or on the cell membrane and all formed reticular strucures attached on cytoskeletons,and Pns6 could also form small aggregates in the cell cytoplasm.
Reticular connective tissue
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