Distribution of opioid peptideom RNA and k-opoid receptors in the limbic system of fawn-hooded and wistar-kyoto rats
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Limbic system
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To specify the functional activity of cannabinoid CB1 receptor in alcohol-preferring Fawn Hooded and alcohol nonpreferring Wistar rats under naïve conditions.Cannabinoid CB1 (WIN-55,212)-stimulated [35S]-GTPgammas binding autoradiography, and cannabinoid CB1 receptor gene expression were measured in rats of both strains that received only water.Cannabinoid CB1 receptor stimulated [35S]-GTPgammas binding was significantly lower in cingulate cortex (Cg), caudate-putamen (CPu), nucleus accumbens (Acc), ventromedial hypothalamic nucleus (VMN), amygdaloid area (AMG), fields (CA1, CA3) of the hippocampus and dentate gyrus (DG) in Fawn Hooded than in Wistar rats, whereas no differences were found either in substantia nigra pars reticulata (SNr) nor CA2 field of the hippocampus. In addition, cannabinoid CB1 receptor gene expression was lower in Cg, CPu, VMN and CA3 field of the hippocampus in Fawn Hooded than in Wistar rats.We speculate that lower cannabinoid function appears to be related to greater vulnerability to alcohol consumption. Cannabinoid CB1 receptor may represent a key target in the treatment of alcohol dependence.
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Caudate nucleus
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Mesolimbic pathway
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Limbic system
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Piriform cortex
κ-opioid receptor
Dynorphin
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The receptor autoradiographic distribution of opioid peptide receptors in spontaneously hypertensive rats (SHR) was compared to that of Sprague-Dawley (SD) rats, using the highly selective μ and δ opioid receptor ligands, [3H]DAGO (Tyr-D-Ala-Gly-NMe-Phe-Gly-ol) and [3H]DPDPE ([D-Pen2,D-Pen5]enkephalin), respectively. Although the distribution of these binding sites was similar in both strains, SHR showed significantly higher binding densities of μ receptors in 16 of 27 areas examined. These included the patch and matrix components of the caudate-putamen (CPu), olfactory tubercle, endopiriform nucleus, anterior cingulate cortex, ventral tegmental area, lateroposteral thalamic nucleus and the ventral part of the dentate gyrus. In contrast, SHR had lower [3H]DAGO binding sites in the CA1 of the hippocampus. Conversely, SHR showed higher binding densities of δ receptors in 7 of 20 areas examined, including the CPu, CA2 and CA3 areas of the hippocampus and the central grey. High-to-low lateromedial gradients of striatal δ receptors were observed in both strains. Because opioid peptides are known to participate in locomotive behavior in rodents and in the control of blood pressure, the present results support a role of opioid peptidergic systems in the manifestation of hyperactivity and hypertension observed in SHR.
μ-opioid receptor
δ-opioid receptor
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Dorsal raphe nucleus
Raphe nuclei
Raphe
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Caudate nucleus
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