Thin hydrogel coatings formation catalyzed by immobilized enzyme horseradish peroxidase
Christian WischkeMarlin KerstingAlexander WelleLiudmila LysyakovaS. BrauneKarl KratzF. JungMatthias FranzrebAndreas Lendlein
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Horseradish peroxidase
Quartz Crystal Microbalance
In this study we show that neurons labelled intracellularly with horseradish peroxidase react differently from surrounding unlabelled neurons in vibratome sections during histological preparations for light and electron microscopy. The diameters and cross‐sectional area of the cell bodies of intracellularly horseradish peroxidase‐labelled neurons increased by about 6 and 11 % respectively, while the surrounding unlabelled neurons decreased by the same amount. Also, the caliber of the proximal dendrites of horseradish peroxidase‐labelled neurons increased during the histological preparation while dendritic path lengths remained unchanged. Since the surrounding tissue shrunk, the dendritic path shapes became more tortuous during histological processing. The demonstrated reaction of horseradish peroxidase‐labelled neurons is suggested to be caused by the horseradish peroxidase reaction product.
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The objective of the present work was to examine biocatalytic properties of peroxidase in horseradish acclimatized in our country. We have found that horseradish root extract’s peroxidase (HRP) has Km 2.5 mM and Vmax 5.36 mM·s-1. Maximum activity (pHopt) was estimated at pH 6.0 and enzyme is more stable in alkali, than in acid. The optimum temperature (Topt) for HRP is 40oC and the enzyme is not stable to temperature influence. The horseradish root’s extract retains enzymatic activity within 21 days. DOI: http://doi.dx.org/10.5564/mjc.v15i0.332 Mongolian Journal of Chemistry 15 (41), 2014, p101-103
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The present study describes an easy and efficient procedure for the purification of horseradish peroxidase from horseradish roots. For this purpose, supermacroporous cryogels having Concanavalin A were prepared by photosensitive cross-linking polymerization. Horseradish peroxidase binding and elution from the prepared cryogels were carried out changing various parameters such as initial peroxidase concentration and pH. The best binding performance was obtained at pH 7.0. The maximum horseradish peroxidase binding of the cryogels was found to be 3.85 mg g −1 cryogel. Horseradish peroxidase purification from crude extract resulted in 115.1-fold. SDS-PAGE analysis and circular dichroism measurements indicated that the horseradish peroxidase purification from horseradish roots was successfully carried out.
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Fluorine-based amphiphobic coatings have been widely used in commercial textiles to provide water- and oil-repelling abilities. However, few reports from the literature survey have discussed the surface structural effects of the coated substrate on amphiphobicity. In this research, various thickness amphiphobic coatings based on mixed epoxy, tetraethylorthosilicate, and a particular alkoxysilane with fluorinated side chains (F-silane) were deposited on Grade 420 stainless steel plates. Film amphiphobicity is characterized by measuring the water and oil contact angles of the coating. Film morphology is examined using atomic force microscopy. The deposited films free of F-silane are thinner than 150 nm. The films become thick at high F-silane volume percentage with the surface cavities, ridges, and granules being masked out. On the addition of F-silane, the water contact angle of the deposited films increases up to 105° and then reaches a plateau of ∼ 107° with increasing F-silane. In contrast, the oil contact angle increases up to 60° at first and then slowly declines with the F-silane concentration. The total drop of oil contact angle by ∼ 20° was attributed to the masking out of surface features on film thickening. This indicates that the surface oleophobicity depends on surface structures. Therefore, improving surface amphiphobicity correlates with creating more refined multiscale surface structures during the industrial manufacturing process of steel plate, prior to surface modification by F-silane. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 41003.
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Horseradish peroxidase(HRP) is a peroxidase with heme-containing enzyme for redox-center, which isolated from horseradish roots. In recent years, HRP could be applied for wastewater treatment and food industry, organic synthesis as well as chemiluminescent assays. The structure, immobilization and application of horseradish peroxidase were introduced significantly. The present problems and the future advance in the industry of HRP were reviewed.
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In this paper we study the possibility to develop an alternative Analytical Method for Investigation in Real‐Time of Liquid Properties, the layout and the operation with Quartz Crystal Microbalance (QCM) Systems. The quartz crystal microbalance (QCM) can be accepted as a powerful technique to monitor adsorption and desorption processes at interfaces in different chemical and biological areas. In our paper, Quartz Crystal Microbalance is used to monitor in real‐time the polymer adsorption followed by azoic dye adsorption and then copolymer adsorption as well as optimization of interaction processes and determination of solution effects on the analytical signal. The solutions of azoic dye (5⋅10−4 g/L, 5⋅10−5 g/L and 5⋅10−6 g/L in DMF) are adsorbed at gold electrodes of QCM and the sensor responses are estimated through decrease and increase of QCM frequency. Also, the response of the sensor at maleic anhydride (MA) copolymer with styrene St (MA‐St copolymer concentration of solution: 5⋅10−4 g/L; 5⋅10−5 g/L and 5⋅10−6 g/L in DMF) is fast, large, and reversible. The detailed investigation showed the fact that the Quartz Crystal Microbalance is a modern method to study a wider number of physical and chemical properties related to the surface and interfacial processes of synthesized copolymer leading to a higher reliability of the research results.
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X-ray photoelectron spectroscopy (XPS) and static contact angle measurements were used to study the interaction between silane coupling agents and cured cement paste. Three different silane coupling agents were investigated: aminopropyltriethoxy silane (APTES), 3-glycidyloxypropyltrimethoxy silane (GPTMS), and methoxy-terminated polydimethxyl siloxane (PDMS). These silanes have different end groups, so the change in surface energy after undergoing a successful reaction between the silane and hydroxyls on the surface of the cement paste was demonstrated by a change in contact angle. Relative to untreated samples, APTES samples decreased the contact angle, PDMS samples increased the contact angle, and GPTMS did not show a significant change in contact angle. Samples with a water-to-cement ratio (w/c) of 0.5 showed a larger change in contact angle than 0.4 w/c ratio samples, because of a greater number of hydroxyl groups at the surface. Deconvolution of the O 1s and Si 2p XPS peaks were performed to determine contributions from bridging and nonbridging atoms. An increase in bridging silicon and oxygen atoms relative to untreated samples indicated successful silane condensation and that a covalent bond was formed between the cement paste and silanes.
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