Sericin and fibroin nanoparticles—natural product for cancer therapy: a comprehensive review
40
Citation
151
Reference
10
Related Paper
Citation Trend
Abstract:
Silk, a natural compound of silkworm contains two proteins, sericin and fibroin. These proteins can be conjugated with other compounds to form silk-derived nanoparticles. The biomedical applications of silk-based nanoparticles for drug delivery and cancer treatment are arising as they are biocompatible, biodegradable, have enhanced permeability and retention effect and less toxicity. Nevertheless, not a single review of literature is present that could describe the anticancer potential of silk derived nanoparticles. In this review, we describe the (i) comprehensive note on fibroin and sericin based nanoparticles (ii) anticancer mechanistic accompanied by biomedical applications in diagnosis, imaging, and drug delivery.Keywords:
Fibroin
Sericin
Natural product
Cancer Therapy
Biocompatible material
The development of a method for the quantitative determination of the sericin content ratio (SCR) is urgently needed for silk refining and the purification of silk fibroin for biomedical applications. In this work, a series of sericin/fibroin mixed samples with known SCRs were prepared by mixing initial samples of extracted sericin and fibroin from Bombyx mori silk. Significant differences were found in the contents of characteristic hydrophilic amino acids abundant in sericin and hydrophobic amino acids abundant in fibroin, and several linear relationships of SCR associated with the content ratios of Ser/Ala, Asp/Ala, Lys/Ala, Asp/Gly and Ser/Gly were established by amino acid analysis. Subsequently, the linear equation expressing SCR (%) as a function of the Asp to Ala content ratio X (%) was established as SCR = 2.5634X − 12.5587 (R2= 0.9972). The results indicated that the SCR of degummed silks calculated by the equation is more objective and effective than the results obtained by the traditional weight loss method. Our study provides a novel approach for the sensitive and quantitative detection of the sericin content within the detection limit in unknown silks, which can contribute to quality control in the silk production process.
Sericin
Fibroin
Alanine
Cite
Citations (2)
Fibroin
Sericin
Antheraea pernyi
Cite
Citations (40)
Silk sericin is the gum-like protein surrounding the fibroin in silk fibers, and generally it is removed during the fiber processing to produce fabrics. Like other silk proteins, the silk of tasar silkworms is also composed of sericin and fibroin. In this study tasar silk sericin was extracted and was confirmed to be a 70 KDa protein by SDS PAGE. The use of sericin in the field of biomedical applications has increased their use for various purposes, the extracted silk sericin was pegylated with activated PEG (MW 5000) and the conjugated product was characterized by FT-IR, 1H NMR, CD, DSC, XRD, and AF TEM and self-assembled nanoparticles were prepared.
Sericin
Fibroin
Cite
Citations (1)
Sericin, one of the two main proteins of silk cocoon besides fibroin, has been wildly used as ingredient in cosmetic products and nutrition supplements; however there are considerable controversial reports on its toxicity to cells and its advantages. This work aimed to investigate cell biocompatibility of sericin both in the systems of blended silk fibroin/sericin and pure sericin films using L929 mouse fibroblasts. The effect of concentration of commercial heat extracted sericin on cell viability was first investigated in the system of silk fibroin/sericin (F/S) films using 2 types of silk fibroin, Bombyx mori Nangnoi Srisaket 1 and Bombyx mori Jul 1/1. For both types of silk fibroin, it was found the lower cell number attached and proliferated on the blended F/S films at all sericin concentrations, compared to that on glass and pure fibroin. However, proliferation rate of cells cultured on the blended F/S films was similar to that of cells cultured on glass and pure fibroin films, as confirmed by population doubling time (PDT). Cytotoxicity of sericin extracted from 4 different methods including heat, acid, alkali and urea treatments was further studied in this work in the system of pure sericin films. It could be seen that acid and urea extracted sericin films showed high percentage of cell attachment at 92% and 88%, respectively. However, number of cells proliferated on all sericin films after 48 h culture was not significantly different. This indicated that L929 cells had different proliferation rate when cultured on different types of sericin films. Among 4 extraction methods, the PDT of cells proliferated on urea extracted sericin film was lowest (27 h) and also lower than that of the blended F/S films. This study suggested that sericin extracted by urea treatment could enhance proliferation rate of L929 mouse fibroblasts.
Sericin
Fibroin
Biocompatibility
Viability assay
Cite
Citations (6)
Understanding the interactions between fibroin and sericin is crucial in solving the mechanism of silk spinning. In this study, various commercially available dyes were used to monitor the interface between fibroin and sericin during the gelation of fibroin. The phase separation between fibroin and sericin could be observed by the addition of azo dyes over a certain molecular weight. Furthermore, the addition of the dyes to the sericin layer showed vivid phase separation over addition to the fibroin layer.
Fibroin
Sericin
Cite
Citations (4)
Sericin
Fibroin
Cite
Citations (213)
Recently, regenerated silk fibroin is one of the most investigated silk-based materials for tissue engineering. However, it needs complex preparation process and will irresistibly denature to solid state with time, which is the biggest challenge to produce in large scale and widely use it as commercial material. In this work, industrially produced sericin, silk fibroin amino acid, and silk fibroin powder were investigated on its physico–chemical and biological properties compared with laboratory prepared regenerated silk fibroin, in order to excavate the potential application in biomedical and therapeutical fields. The results showed that sericin may be the most suitable silk materials to use as an alternative in tissue engineering compared with regenerated silk fibroin. It was found that sericin can absorb UV in the range of 223–300 nm, inhibit tyrosinase, resist to free radicals, and retain water capacity similar to hyaluronic acid. Importantly, cell experiment on human fibroblast showed that sericin (0.05 wt%) could stimulate cell proliferation and obviously up-regulate the expression of collagen type I, collagen type III, and hyaluronidase I compared with the control at seven days, which is the closest equivalent to regenerated silk fibroin. These results suggested that sericin might be a valuable ingredient for skin repair on tissue engineering.
Fibroin
Sericin
Cite
Citations (35)
In this paper silkworm cocoon was dissolved at different time by degumming agents of NaHCO3 to obtain silk fibroins with different content of sericin. Then the silk fibroins was dissolved to get the silk fibroin membranes. The results indicated that the content of sericin had certain effect on silk solubility. As the content of sericin reduced, the dissolution speed was faster and the silk solubility was greater. The properties of the silk fibroin membranes with some sericin were much better than those degummed completely.
Fibroin
Sericin
Cite
Citations (0)
Sericin, a fascinating natural biomaterial derived from silkworms, has received increasing interest in recent years for its unique bioactivity and high compatibility. Silkworms can be divided into wild-type or silk fibroin-deficient mutants according to whether they synthesize and secrete silk fibroin. Silk fibroin-deficient mutant silkworms and their cocoons are convenient for us to obtain diverse and high-quality sericin, which has been applicated in various fields such as cell culture, tissue engineering, drug delivery, and cosmetics. Here, we present an overview of our silkworm varieties resources, especially silk fibroin-deficient mutant silkworms. We optimized various extraction methods of sericin and summarized the characteristics and advantages of sericin. Finally, we developed and discussed a series of sericin-based biomaterials for promising applications for a diverse set of needs.
Fibroin
Sericin
Biomaterial
Cite
Citations (21)