Antioxidant Effect of the Lactobacillus Extracts on Aging Mice Model
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Study the antioxidant effect of the Lactobacillus extracts on D-galactose induced aging mice model. This paper has chosen Kunming mice as the object. While the mice were injected with D-galactose, they were also treated with different Lactobacillus extracts respectively through oral gavage. Moreover, after 6 weeks,the animal experiment indicated that compared with the control, the antioxidant functions of serum and liver in normal and Lactobacillus extract mice are significantly improved through enhancing SOD,GSH-Px,CAT and T-AOC activitiesand and reducing MDA contents in mouse serum and liver tissue.Keywords:
Animal model
Liver tissue
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Abstract Yogurt from Xinjiang, China, is a traditional Chinese fermented food rich in beneficial microorganisms, such as Lactobacillus plantarum KSFY06. In this study, the effect of KSFY06 on oxidative aging was investigated using live animal experiments. Molecular biological methods were used to analyze the serum and tissues of mice with oxidative aging induced by d ‐galactose, which showed that KSFY06 can inhibit the decline of heart, liver, spleen, and kidney caused by aging. The KSFY06 strain increased the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), catalase (CAT), and glutathione (GSH) in serum and liver of aging mice, while the content of malondialdehyde (MDA) is reduced. Pathological observation showed that KSFY06 alleviated damage to the liver, spleen, and skin of oxidative aging mice. qPCR showed that, at high dose (2 × 10 9 cfu/kg per day), KSFY06 upregulates copper/zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase (nNOS), catalase (CAT) mRNA expression, and its downstream inducible nitric oxide synthase (iNOS) mRNA expression in liver and spleen tissues induced by d ‐gal. To a certain extent, these findings indicate that L. plantarum KSFY06 is able to protect against oxidative stress in the d ‐gal‐induced aging model. In conclusion, L. plantarum KSFY06 may provide a potential research value in the prevention or alleviation of related diseases caused by oxidative stress.
Malondialdehyde
SOD2
Dismutase
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Oxidative stress, one of the most common biological dysfunctions, is usually associated with pathological conditions and multiple diseases in humans and animals. Chinese olive fruit ( Canarium album L.) extracts (OE) are natural plant extracts rich in polyphenols (such as hydroxytyrosol, HT) and with antioxidant, anti-hyperlipidemia, and anti-inflammatory potentials. This study was conducted to investigate the antioxidant capacity of OE supplementation and its related molecular mechanism in mice. Mice (25.46 ± 1.65 g) were treated with 100 mg/kg body weight (BW) OE or saline solution for 4 weeks, and then the antioxidant and anti-inflammatory capacities of mice were examined. The results showed that OE supplement significantly increased the serum antioxidative enzyme activities of total antioxidant activity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase and decreased the serum malondialdehyde (MDA) level, indicating that OE treatment enhanced the antioxidant capacity in mice. qPCR results showed that the transcriptional expression of antioxidant SOD1, CAT, Gpx1 , and Gpx2 were significantly down-regulated in the small intestine (jejunum and ileum) after OE administration. Meanwhile, OE treatment significantly decreased the T-AOC and increased the MDA level in the small intestine. Furthermore, OE administration dramatically reduced the mRNA expression of pro-inflammatory cytokines (TNF-α and IL-1β), which confirmed its antioxidant and anti-inflammatory capacities with OE administration. Using amplicon sequencing technology, 16S rRNA sequencing results showed that OE supplement significantly increased the colonic Firmicutes / Bacteroidetes ratio, which also had a negative correlation with the serum MDA level and positively correlated with serum GSH-Px activity through Pearson correlation analysis. Besides that, Alloprevotella was negatively correlated with serum T-AOC. Colidextribacter was positively correlated with serum MDA and negatively correlated with serum T-AOC, SOD, and GSH-Px levels. In summary, this study showed that treatment with 100 mg/kg BW polyphenol-rich OE could alter colonic microbiota community, which was strongly associated with improved antioxidant capacity in mice.
Hydroxytyrosol
Malondialdehyde
GPX1
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Subcutaneous injection
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Mice were administered subcutaneous injection of D-galactose to produce an animal model of aging, which was used to study the in vivo effect of fermented plant extract(FPE) on antioxidant activity. Parameters including the activity of three characteristic antioxidant enzymes, namely superoxide dismutase(SOD), catalase(CAT), and glutathione peroxidase(GSH-Px) as well as the malondialdehyde(MDA) content in the serum and liver of mice of both sexes were extensively evaluated. Meanwhile, spleen index and thymus index were used as the indicators for the preliminary assessment of the impact of FPE on mice immunity in vivo. The results showed that compared with the model group, the activities of SOD, CAT, and GSH-Px in serum and liver of mice(both sexes) were significantly enhanced and corresponding MDA content was decreased in the group that received 1.5 m L/kg FPE gavage. Additionally, spleen and thymus indices of mice were increased to some extent in groups that received 0.24 and 0.6 m L/kg FPE gavage. Thus, FPE enhanced antioxidant activities in vivo, implying an anti-aging effect and may have potential to strengthen the immune system.
Malondialdehyde
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Objective To study the mechanism of anti-oxidative activity and the anti-oxidative effect of gynostemma and hawthorn extracts on aging mouse model.Methods The aging mice were induced by ip injection of D-galactose for 8 weeks and were given the different doses of gynostemma and hawthorn extracts.Eight weeks later,malondialdehyde(MDA),superoxide dismutase(SOD) and glutathione peroxidase(GSH-P_X) in sera and livers were detected.Results Gynostemma and hawthorn extracts not only significantly reduced the contents of MDA but also increased the activity of GSH-P_X in sera and livers of the aging mice.Conclusion Gynostemma and hawthorn extracts have good anti-oxidative effect.
Malondialdehyde
Gynostemma pentaphyllum
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This study was undertaken to investigate the effects of the extracts of buckwheat leaf and flower on the antioxidant status of the brain and liver tissue. The administration of buckwheat extracts (both concentrations were 10%) to mice (at the dose 10 mL/kg of body weight) for 21 days significantly decreased superoxide dismutase (SOD) activity and reduced the amount of glutathione (GSH) and malondialdehyde (MDA) in the mouse brain, while catalase (CAT) activity significantly increased. In the mouse liver, the amount of GSH and activity of SOD increased, while the CAT activity after administering buckwheat leaf and flower extracts was lower in experimental mice than in the control group. However, the administration of 10% ethanol (for 21 days) to control animals also had a significant effect on the antioxidant system in brain and liver cells. Experimental animals demonstrated rather marked changes in the activities of the antioxidant enzymes SOD and CAT in their liver and brain cells, and changes in the levels of GSH and MDA were observed when compared with the control group.
Malondialdehyde
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Antioxidant effect of rice peptide was studied through the model of aging mice induced by D galactose.CAT, MDA and SOD in serum of aging mice were observed.Results showed 400mg/kg rice peptide could significantly improve the vitali of CAT (p<0.05) and SOD (p<0.05) in the aging mice serum, reduce the content of MDA (p<0.01) .Rice peptide could suppress symptoms of aging on mice by antioxidant effects.
Senescence
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Abstract Background Excessive reactive oxygen species (ROS) can cause serious damage to the human body and may cause various chronic diseases. Studies have found that lactic acid bacteria (LAB) have antioxidant and anti-aging effects, and are important resources for the development of microbial antioxidants. This paper was to explore the potential role of an antioxidant strain, Lactobacillus plantarum NJAU-01 screened from traditional dry-cured meat product Jinhua Ham in regulating D-galactose-induced subacute senescence of mice. A total of 48 specific pathogen free Kun Ming mice (SPF KM mice) were randomly allocated into 6 groups: control group with sterile saline injection, aging group with subcutaneously injection of D-galactose, treatments groups with injection of D-galactose and intragastric administration of 10 7 , 10 8 , and 10 9 CFU/mL L. plantarum NJAU-01, and positive control group with injection of D-galactose and intragastric administration of 1 mg/mL Vitamin C. Results The results showed that the treatment group of L. plantarum NJAU-01 at 10 9 CFU/mL showed higher total antioxidant capacity (T-AOC) and the antioxidant enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) than those of the other groups in serum, heart and liver. In contrast, the content of the oxidative stress marker malondialdehyde (MDA) showed lower levels than the other groups (P < 0.05). The antioxidant capacity was improved with the supplement of the increasing concentration of L. plantarum NJAU-01. Conclusions Thus, this study demonstrates that L. plantarum NJAU-01 can alleviate oxidative stress by increasing the activities of enzymes involved in oxidation resistance and decreasing level of lipid oxidation in mice.
Malondialdehyde
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[Objective] To investigate anti-lipid peroxidation of Zingiber extract with alcohol in the subacute aging mice induced by D-galactose.[Methods]The aging mice were induced by D-galactose.Meanwhile,they were treated with two doses of Zingiber extract.The contents of MDA and the activity of SOD and GSH-Px in serum,liver and brain were determined when the mice were killed after 6 weeks.[Results]Zingiber extract could significantly improve the activity of SOD and GSH-Px,and decrease the contents of MDA in serum,liver and brain.[Conclusions]Zingiber extract has good anti-aging effects on the aging mice,which is probably due to its effects of improving antioxidation,eliminating free radicals and prohibiting lipid peroxidation.
Zingiber officinale
Lipid peroxide
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