Lactobacilli modulated AMPK activity and prevented telomere shortening in ageing rats
Lee-Ching LewYan-Yan HorMohd Hafiidz JaafarAmy Sie-Yik LauJia-Sin OngLi Oon ChuahKien Pong YapGhows AzzamAzali AzlanM.T. Liong
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This study aimed to evaluate the anti-ageing effects of different strains of lactobacilli putative probiotics on an ageing rat model as induced by D-galactose and a high fat diet. Male Sprague-Dawley rats were fed with high fat diet (54% kcal fat) and injected with D-galactose daily for 12 weeks to induce ageing. The effects of putative probiotic strains on age-related impairment such as telomere length, plasma lipid peroxidation, hepatic 5'adenosine monophosphate-activated protein kinase (AMPK) expression, as well as endurance performance were evaluated. Administration of statin, Lactobacillus plantarum DR7 (LP-DR7), Lactobacillus fermentum DR9 (LF-DR9), and Lactobacillus reuteri 8513d (LR-8513d) significantly reduced the shortening of telomere and increased the expression of AMPK subunit-α1 (P<0.05). Plasma lipid peroxidation was lower (P<0.05) in groups administered with statin and LF-DR9 as compared to the control. AMPK subunit-α2 was elevated in rats administered with LP-DR7 as compared to the control (P<0.05). Using an in vivo ageing rat model, the current study has illustrated the potentials of lactobacilli putative probiotics in alleviation of age-related impairment in a strain-dependent manner.Keywords:
Lactobacillus reuteri
Objective To study the composition of Lactic acid bacteria in the intestine of healthy ICR mice.Methods Fresh fecal samples from 10 healthy male ICR mice were collected and the total genomic DNA was extracted from each sample.Lactic acid bacteria(LAB) group specific primer(Lac1) and universal primer for bacteria(1391r) were used to amplify 16S rRNA gene and construct LAB group specific clone library to analyze the species composition of LAB.Results The results of clone library showed that Lactobacillus reuteri and Lactobacillus johnsonii were the most predominant species in gut of mice.Lactobacillus murinus,Lactobacillus vaginalis,Lactobacillus intestinalis and a potentially new species were also detected.Conclusion The composition and structure of Lactobacillus in healthy ICR mice have high diversity and the species of Lactobacillus reuteri may have high microdiversity at strain level.
Lactobacillus reuteri
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ABSTRACTAn interspecies genetic recombination by fusion of protoplasts of antibiotic-resistant clones of lactobacilli: Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus helveticus, Lactobacillus casei, and Lactobacillus plantarum has been accomplished. It has been shown that the conditions of degradation of the lactobacilli cell wall allow a high relative share of formed protoplasts to be achieved. After the protoplast fusion and regeneration of the cell wall a heterogenic population is obtained. A series of recombinant clones has been selected, which differ from the parent cultures in improved or new qualities, such as higher reproducibility, acid-forming ability, assimilation and fermentation of a greater number of carbon sources, phage-resistance etc. Among them have been selected the recombinant strains—Lactobacillus LAB 8, Lactobacillus LHC 28, Lactobacillus LBC 7, Lactobacillus LBC 16, Lactobacillus LPC 12, Lactobacillus LPC 48, Lactobacillus LPC 51.
Lactobacillus casei
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Lactobacillus helveticus
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Effect of hot water extract (HWE) from Fructus tribuli on growth of Lactobacillus casei , Lactobacillus reuteri , Lactobacillus rhamnosus and Lactobacillus bulgaricus with material properties was studied by measuring optical density at 600nm (OD 600 ) and pH using MRS media as the control. The addition of HWE (v/v) was 1%, 2%, 3%, 4% and 5%. Results were as follows: addition of HWE has the significant inhibition on growth of Lactobacillus reuteri and Lactobacillus bulgaricus and the significant promotion on growth of Lactobacillus casei and Lactobacillus rhamnosus . The optimum concentration of HWE in MRS media was 2% for Lactobacillus casei and 3% for Lactobacillus rhamnosus , respectively.
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Lactobacillus reuteri
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Modern molecular genetic methods of research, which emerged in the 21st century, completely unfixed all established notions not only about the microcosm, but also about its influence on the life and health of the macroorganism. The identification of intestinal bacteria and particularities of their various strains is increasingly expanding our understanding of the mechanisms of their action on the somatic and mental health of a human being. The various lactobacilli strains have been the most studied probiotic at the present time. The genus Lactobacillus is known to include about 90 species with a wide range of biochemical and physiological properties. Lactobacillus reuteri, which has strains with different mechanisms of action, is one of the unique microorganisms of the family Lactobacillaceae. The range of possibilities for using various Lactobacillus reuteri strains is currently very wide. This article deals with clinical effects associated with the strain-specificity of Lactobacillus reuteri. Современные молекулярно-генетические методы исследования, появившиеся в XXI в., полностью перевернули наши представления не только о микромире, но и о его влиянии на жизнь и здоровье макроорганизма. Идентификация кишечных бактерий и особенности их разных штаммов все больше расширяют наше понимание механизмов их влияния на соматическое и психическое здоровье человека. На сегодняшний день наиболее изученными являются различные штаммы лактобацилл. Известно, что род Lactobacillus включает примерно 90 видов с широким спектром биохимических и физиологических свойств. Одним из уникальных микроорганизмов семейства Lactobacillaceae является Lactobacillus reuteri, имеющая штаммы с разным механизмом действия. На сегодняшний день спектр возможностей использования разных штаммов Lactobacillus reuteri очень широкий. В данной статье рассмотрены клинические эффекты, связанные со штаммоспецифичностью Lactobacillus reuteri.
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To analyze the microbiota shift in the distal esophagus of Sprague-Dawley rats fed a high-fat diet.Twenty Sprague-Dawley rats were divided into high-fat diet and normal control groups of 10 rats each. The composition of microbiota in the mucosa from the distal esophagus was analyzed based on selective culture. A variety of Lactobacillus species were identified by molecular biological techniques. Bacterial DNA from Lactobacillus colonies was extracted, and 16S rDNA was amplified by PCR using bacterial universal primers. The amplified 16S rDNA products were separated by denaturing gradient gel electrophoresis (DGGE). Every single band was purified from the gel and sent to be sequenced.Based on mucosal bacterial culturing in the distal esophagus, Staphylococcus aureus was absent, and total anaerobes and Lactobacillus species were decreased significantly in the high-fat diet group compared with the normal control group (P < 0.01). Detailed DGGE analysis on the composition of Lactobacillus species in the distal esophagus revealed that Lactobacillus crispatus, Lactobacillus gasseri (L. gasseri) and Lactobacillus reuteri (L. reuteri) comprised the Lactobacillus species in the high-fat diet group, while the composition of Lactobacillus species in the normal control group consisted of L. gasseri, Lactobacillus jensenii and L. reuteri.High-fat diet led to a mucosal microflora shift in the distal esophagus in rats, especially the composition of Lactobacillus species.
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The intact cells,cell-free extract and inactive cells of the Lactobacillus plantarum and Lactobacillus bulgaricus's antioxidative capacity were studied.The scavenging capacity of DPPH,hydroxyl radical,and superoxide anion,inhibition of lipid peroxidation,the reducing activity and chelation capacity of metal ions were examined,and the difference between the two Lactobacillus was compared.The results showed that Lactobacillus plantarum's antioxidative capacity was higher than Lactobacillus bulgaricus',which provided the foundation of comprehensive utilization for the Lactobacillus plantarum.
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Seven novel lactic acid bacterial strains, isolated from traditional Chinese pickle, were characterized using a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, determination of DNA G+C content, determination of average nucleotide identity (ANI), in silico DNA–DNA hybridization (isDDH), fatty acid methyl ester (FAME) analysis and an analysis of phenotypic features. Strains 382-1T, 116-1AT, 381-7T, 203-3T, 218-3T and 398-2T were phylogenetically related to the type strains of Lactobacillus plantarum subsp. plantarum , Lactobacillus plantarum subsp. argentoratensis , Lactobacillus pentosus , Lactobacillus paraplantarum , Lactobacillus fabifermentans , Lactobacillus herbarum , Lactobacillus mudanjiangensis , Lactobacillus xiangfangensis , Lactobacillus plajomi and Lactobacillus modestisalitolerans , having 97.1–99.9 % 16S rRNA gene sequence similarities, less than 89.9 % pheS gene sequence similarities, less than 98.0 % rpoA gene sequence similarities, less than 91.2 % ANI values and less than 43.3 % isDDH values. Strain 778-3T was phylogenetically related to the type strains of Lactobacillus hokkaidonensis , Lactobacillus wasatchensis , Lactobacillus oligofermentans , Lactobacillus nenjiangensis , Lactobacillus vaccinostercus and Lactobacillus suebicus , exhibiting 97.0–99.4 % 16S rRNA gene sequence similarities, 78.2–82.1 % pheS gene sequence similarities, 80.0–91.5 % rpoA gene sequence similarities, less than 78.6 % ANI values and less than 22.9 % isDDH values. Based upon the data of polyphasic characterization obtained in the present study, seven novel species, Lactobacillus pingfangensis sp. nov., Lactobacillus daoliensis sp. nov., Lactobacillus nangangensis sp. nov., Lactobacillus daowaiensis sp. nov., Lactobacillus dongliensis sp. nov., Lactobacillus songbeiensis sp. nov. and Lactobacillus kaifaensis sp. nov., are proposed and the type strains are 382-1T (=NCIMB 15187T=CCM 8935T=LMG 31176T), 116-1AT (=NCIMB 15181T=CCM 8934T=LMG 31171T), 381-7T (=NCIMB 15186T=CCM 8930T), 203-3T (=NCIMB 15183T=CCM 8933T=LMG 31172T), 218-3T (=NCIMB 15184T=CCM 8932T=LMG 31173T), 398-2T (=NCIMB 15189T=CCM 8931T=LMG 31174T) and 778-3T (=NCIMB 15191T=CCM 8929T=LMG 31177T), respectively.
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Lactobacillus reuteri
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Cecum
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سویههای پروبیوتیکی Lactobacillus قابلیت استفاده به عنوان نگهدارنده غذایی را دارند. پژوهش حاضر با هدف بررسی استفاده از Lactobacillus delbrueckii subsp. bulgaricus (PTCC 1737)، L. reuteri (PTCC 1655)، L. brevis CD0817 و ترکیب باکتریهای Lactobacillus روی کیفیت میکروبی فیله قزلآلای رنگینکمان در یخچال و انجماد انجام شد. تیمارها شامل فیلههای غوطهور شده در سوسپانسیون L. reuteri، L. brevis، L. delbrueckii و ترکیب آنها بودند. فیلههای غوطهور شده در اسید لاکتیک ۲ درصد و فیلههای بدون افزودنی به عنوان تیمارهای شاهد در نظر گرفته شدند. تیمارها به مدت ۵ روز در یخچال و شش ماه در انجماد نگهداری شدند. Staphylococcus aureus و Escherichia coli در تیمارهای آزمایشی و شاهد مشاهده نشدند.L. delbrueckii قادر به بقا تحت شرایط اتجماد نبود. تعداد Lactobacillusها در تیمارهای ترکیبی طی مدت زمان نگهداری در شرایط انجماد کاهش داشت (logCFU/g۳۴/۱). در L. brevis اسیدهای چرب اشباع ۹۱/۲۳ درصد و غیراشباع ۷۷ درصد و در تیمار L. reuteri اسیدهای چرب اشباع ۱۲/۲۷ درصد و غیراشباع ۸۸/۷۲ درصد بود (05/0P<). افزایش معنیدار در نسبت اسیدهای چرب غیراشباع به اشباع در L. brevis در مقایسه باL. reuteri و عدم تفاوت معنیدار در تعداد L. brevis طی مدت زمان نگهداری در دمای انجماد سبب شد که L. brevis به عنوان محافظ غذایی برای نگهداری فیله قزلآلای رنگینکمان پرورشی در یخچال و انجماد پیشنهاد شود.
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Probiotics that are able to provide beneficial effects on animal health have become important ingredients of dog foods. This study was conducted to characterize the probiotic potentials of two strains, Lactobacillus reuteri BCLR-42 and Lactobacillus plantarum BCLP-51, that were derived from feces of healthy dogs and evaluated based on tolerance to low pH and bile acid, antimicrobial activities, enzyme profiles, sensitivity to antibiotics, and innate immune enhancing potentials. Both strains showed survival of more than 90% at pH 3 and 0.2% bile acid and exhibited broad antimicrobial activities against indicator bacteria. Moreover, both strains showed high sensitivity to antibiotics, except vancomycin, metronidazole, and gentamicin. The alkaline phosphatase was negligible (score 0), whereas they showed strong beta galactosidase activity (score range 5 or 3, respectively). The phagocytosis and oxidative burst activities of canine granulocytes were significantly enhanced in response to both strains. These results show that both strains have the capability to act as probiotics and the potential for application as ingredients in dog foods.
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