Preparation of multifunctional PLZT nanowires and their applications in piezocatalysis and transparent flexible films
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Electrospinning
Polydimethylsiloxane
Methyl orange
Dielectrophoresis
Dielectrophoresis
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Abstract Poly(hydroxybutyrate‐ co ‐hydroxyvalerate) (PHBV) was electrospun into ultrafine fibrous nonwoven mats. Different from the conventional electrospinning process, which involves a positively charged conductive needle and a grounded fiber collector (i.e., positive voltage (PV) electrospinning), pseudo‐negative voltage (NV) electrospinning, which adopted a setup such that the needle was grounded and the fiber collector was positively charged, was investigated for making ultrafine PHBV fibers. For pseudo‐NV electrospinning, the effects of various electrospinning parameters on fiber morphology and diameter were assessed systematically. The average diameters of PHBV fibers electrospun via pseudo‐NVs were compared with those of PHBV fibers electrospun via PVs. With either PV electrospinning or pseudo‐NV electrospinning, the average diameters of electrospun fibers ranged between 500 nm and 4 μm, and they could be controlled by varying the electrospinning parameters. The scientific significance and technological implication of fiber formation by PV electrospinning and pseudo‐NV electrospinning in the field of tissue engineering were discussed. POLYM. ENG. SCI., 2011. © 2011 Society of Plastics Engineers
Electrospinning
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AC-dielelectrophoresis is utilized inside a lab-on-a-chip device to separate particles and cells. Dielectrophoresis is the movement of particles in a non-uniform electric field due to the interaction of the particle's dipole and spatial gradient of the electric field. Dielectrophoresis is a subtle solution to manipulate particles and cells at microscale due to its favorable scaling for reduced size of the system. Dielectrophoresis is applicable with both DC and AC fields. DC-dielectrophoresis only depends on the electrical conductivities of the particle and the medium. AC-dielectrohoresis depends on the permittivities of the particle and the medium, and the field frequency. AC-dielectrophoresis is richer in the sense that both positive and negative dielectrophoretic force can be generated for biological particles by tuning the field frequency. The dielectrophoretic force depends on the size and the electrical properties of the particles and the suspending medium which makes the separation of particles and cells based on their size and based on their electrical properties possible. In this dissertation, the continuous separation of particles and cells based on their size and based on their electrical properties is achieved inside a lab-on-a-chip device. PDMS (polydimethylsiloxane) microchannels are fabricated using soft lithography technique. The flow is induced by pressure gradient. Simple, 3D electrodes which are fabricated by a simple and inexpensive technique extended from soft-lithographic fabrication are used to achieve a localized, non-uniform electric field. Dielectrophoretic force is generated in the transverse direction to the flow by inserting 3D electrodes along the channel side walls. The localized electric field is important to reduce the Joule heating and any adverse effects on biological particles due to the interaction of particles with the electric field. Latex particles of different size and mixture of white blood cells (which have a typical size of 8-12 micron) and yeast cells (which have a typical size of 3-5 micron) is separated based on their size difference. The separation based on electrical properties is demonstrated by means of the separation of 10 micron latex particles and white blood cells. A numerical simulation based on Lagrangian tracking method is used to simulate the particle trajectories. The present designs have the feature of using simple electrodes like DC-dielectrohoretic devices and of using low electrical potential like AC-dielectrophoretic devices; they are unique in a sense that the effect of the electric field is confined in a small area which means a very short time for the interaction of the particles with the electric field.
Dielectrophoresis
Microscale chemistry
Polydimethylsiloxane
Particle (ecology)
Lab-on-a-Chip
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The expanding field of AC Electrokinetics offers a number of opportunities for the separation and characterisation of cells. Here the authors consider two methods, Dielectrophoresis and Travelling Wave Dielectrophoresis. Dielectrophoresis is a non-invasive technique that has been used to probe the internal dielectric properties of single cells by inducing a motive force on that cell. By controlling the conditions so that the force exerted on particles of different types is significantly different, a mixture of different cell types can be sorted into groups. Travelling Wave Dielectrophoresis is better able to discriminate small changes in cell properties than conventional Dielectrophoresis. It is also used as a means of transporting cells around electrode arrays. Here the authors illustrate how these technologies and others can be integrated to form an on-chip cell sorter capable of performing Dielectrophoresis Activated Cell Sorting (DACS).
Dielectrophoresis
Cell Sorting
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After rigorous development over the years, dielectrophoresis has been established as an effective method to manipulate micron and sub-micron sized particles.In particular, it is a promising technology for lab-on-a-chip or micro total analysis system (µTAS) to separate cells for biomedical applications.This technology is based on the knowledge that a particle suspended in a fluid medium experiences a net electrical force, due to a polarization effect, when non-uniform electrical fields are applied across the fluid.By varying the applied electric field frequencies, the magnitude and the direction of the dielectrophoretic forces on the particle can be varied and controlled.When the applied electric field only varies in magnitude over time, the dielectrophoretic force is 1-dimensional.This is commonly referred as conventional dielectrophoresis.When the applied electric field has a varying magnitude and phase, the dielectrophoretic force is 2-dimensional.This is commonly referred as traveling wave dielectrophoresis.While particle separations have been demonstrated with devices based on these two techniques, the separated particles were confined in space.To overcome this issue, fluid flow is generally used to carry the particles.In this investigation, moving dielectrophoresis (mDEP) is introduced for the manipulation and transportation of particles.The moving dielectrophoresis is generated by a series of electrodes which can be individually energized to induce an electric field that moves from one electrode to another.Beside the electric field frequency, the switching speed of the electrode is a second time parameter introduced in moving dielectrophoresis.A major difference of this technique from the traveling wave dielectrophoresis is that the moving speed of the energized electrodes is independent of the electric field frequency.By sequentially energizing the electrodes, a particle can be controlled to move in the same direction.By controlling the electric field frequencies and the energizing of the electrodes, other manipulation techniques like separation, isolation, fractionation and trapping can be achieved.A mathematical model is also presented to provide a theoretical basis for the use of the moving dielectrophoresis.
Dielectrophoresis
Particle (ecology)
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The electrodeless dielectrophoretic trapping and concentration of viruses was demonstrated. Dielectrophoresis is the motion of matter caused by polarization effects in a nonuniform electric field. Experiments were performed on a glass chip with insulating posts in order to study the dielectrophoretic behavior of the viruses and extend the application of insulative (electrodeless) Dielectrophoresis (iDEP) to polymeric microdevices. The ultimate goal of his research project is to create a highthroughput EDEP system using polymers as substrate. Only two electrodes were present in the system. In the presence of an applied DC electric field the viruses exhibited streaming and trapping dielectrophoresis.
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我们建议了制作在这的 microfluidic 薄片聚合物主人模子糊的 polydimethylsiloxane (PDMS ) 的一个新奇方法。方法主要包括二步。首先,不锈钢片是蚀刻形成一个金属模型的激光。然后,器官的解决方案(甲基 methacrylate )(PMMA ) poly 是 casted 到金属模型上制作将随后被用来制作 PDMS 芯片的 PMMA 主人。我们系统地研究了影响 microchannels 的表面地位的不同激光参数并且获得了优化蚀刻的参数。当扔的薄片掌握时,我们调查了并且优化,并且开发了一个方法用二个不同粘性答案形成好聚合物主人接着扔模型,并且学习可重复的复制 PMMA 的器官的答案作文。然后,我们调查了这块芯片的物理性能并且由分析玫瑰精 B 评估了有实行可能。与现在的方法相比,建议方法不在 photoresistant 并且化学蚀刻上需要影印石版术。全部制作进步简单,快便宜并且能容易被控制。仅仅几分钟被要求为 PDMS 薄片做一个金属模型,为一位 PMMA 主人的 3 个小时,和一天。
Polydimethylsiloxane
PDMS stamp
Microfluidic chip
Polymethyl methacrylate
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A procedure is described for making layer-to-layer interconnections in polydimethylsiloxane (PDMS) microfluidic devices. Thin (∼50 μm) perforated PDMS membranes are bonded to thicker (0.1 cm or more) PDMS slabs by means of thermally cured PDMS prepolymer to form a three-dimensional (3D) channel structure, which may contain channel or valve arrays that can pass over and under one another. Devices containing as many as two slabs and three perforated membranes are demonstrated. We also present 3D PDMS microfluidic devices for display and for liquid dispensing.
Polydimethylsiloxane
PDMS stamp
Prepolymer
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Dielectrophoresis
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Electrospinning
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