Analysis of Resistance to Blast and Mapping of Rice Blast Resistance Gene in DH104
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The DH104,an indica rice mutant from Huhan B,and eight monogenic lines were subjected to resistance spectrum with 39 isolates collected from Guangdong Province,China.The results showed that DH104 conferred broader resistance spectrum than the eight monogenic lines.To identify and map the blast resistance gene in DH104,a representative isolate GD0193 was used to identify and map R gene from DH104.Isolate GD0193 was used for inoculating a population of F2 derived from the cross between DH104 and highly susceptible variety L101(indica).The results showed that the segregation of resistant(R) and susceptible(S) progenies fitted a 3∶1 ratio in the F2 population(313R∶97S,X2=0.896 1),indicating the R gene was controlled by a single dominant gene,temporarily designed as Pixt(t) gene.The R gene was located between RM136 and RM7213 on chromosome 6 using SSR markers,with genetic distances 4.9 and 1.5 cM respectively.Cite
Pyricularia
Inbred strain
Major gene
Genetic linkage
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Rice blast, caused by fungus Magnaporthe grisea, is one of the most serious diseases of rice and has become a serious threat to food security. Yuanbaozhan is a new rice material. Improving the rice resistance to rice blast through genetic improvement methods is the most effective and safe way in the present. Yuanbaozhan has an broad-spectrum resistance to rice blast pathogen, and it has high resistance to 23 strains among 25 blast strains collected from different regions in Guangdong province. Its resistance frequency is 92%. In this study, we made a combination to establish genetic population F1, F2among which population is from the crosses between Yuanbaozhan and Yuexiangzhan. Evaluation was conducted with Magnaprthe oryzae isolate GD00-193a, the results showed that the resistant and susceptible plants in F2population fitted the ratio of 3:1, indicating that the resistance of Yuanbaozhan to the rice blast was controlled by a dominant gene or a QTL locus. With the bulked-extrems and recessive-class approach analysis, the rice blast resistance gene was mapped at the genome region between RM136 and RM549.
Oryza
Genetic Analysis
Blast disease
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The establishment of molecular marker of rice blast resistance genes was a powerful strategy in resistance to rice blast breeding.In the present study,seventy-one strains in Digu variety was used to check the regional rice blast resistance in Guangxi.A gene tagging M-Pid2 was amplified by the primers designed with the sequence of resistance gene itself,using a base difference between Pi-d2 gene in Digu variety and Guanghui 998 allele.The validity of the gene tag was tested in a F2 generation derived from Digu(a resistant variety including Pi-d2) and Guanghui 998(a susceptible restore line) and a group of germplasm containing 62 rice germplasm.The resistance spectrum was detected by inoculating Pi-d2 gene sponsor with 71 rice blast pathogen strains collected from Guangxi.The resistance spectrum of all materials were also detected in natural disease nursery located in Cenxi county.The results showed that the resistance indicated that Pi-d2 gene tag was highly consistent with that indicated under field detection.Furthermore,PCR analysis indicated that,among the 62 rice germplasm,a specific band of 629 bp was amplified only in Digu variety.It was concluded that rice blast resistance gene Pi-d2 was the main-effect blast resistance genes of Digu rice.and the gene tag could be credibly used in marker assisted selection and germplasm differentiation.
Germ plasm
R gene
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Blast disease
Inheritance
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LongS is a dual purpose genic male sterile rice with broad-spectrum resistance to rice blast. The objective of the present study was to identify the resistance spectrum to rice blast, to analyze the genetic behavior of resistance gene, and to map the major resistance genes in LongS. LongS had a resistance frequency of 100% inoculated with 41 strains of Magnaporthe oryzae. Population genetic analysis showed that the resistance genes in LongS exhibited dominant inheritance, the genetic model of R gene varied depend on the strains of Magnaporthe oryzae. The main-effect resistant gene to rice blast was fine mapped, by using the bulk segregant analysis (BSA) and recessive class analysis (RCA) methods, with the F2 population derived from the resistant parent of LongS and the susceptible parent of Nipponbare. A single resistant gene to the race of 318-2 located on the interval flanked by the SSR markers of M1 and M2 with a genetic distance of 1.3 cM on chromosome 9 were adjacent to the broad-spectrum blast resistance gene, Pi5. Both of the resistance spectrum and resistant frequency of LongS, however, were significantly different to those of resistant gene of Pi5 and Pii. In conclusion, the major-effect resistant gene identified in this study may be a new broad-spectrum blast resistance gene. The DNA markers linked to the new R gene identified in this study should be useful for marker-aided breeding of blast-resistant rice cultivars.
Genetic Analysis
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The rice blast resistance(R) could be evaluated entirely by investigating the resistance spectrum at seedling stage and neck blast resistance at adult stage.In this study,three space-induced rice mutant lines,which had been screened primarily,were performed with resistance spectra with 36 blast isolates from Guangdong Province and neck blast resistance in a natural blast nursery,Lutian,Conghua.The results indicated that the mutant lines conferred broad resistance spectra and high level of resistance to neck blast,while the on-ground control was highly susceptible.Genetic analysis showed that a single major dominant R gene conditioned H4's and H11's resistance to isolates GD0193 and GD866,and there were two independent dominant R genes in H4 and H11 corresponding to isoaltes GD08T4 and GD08T13,respectively.The inherited pattern of resistance in D69 to isolates GD0193 was complicated,while a single dominant major R gene controlled its resistance to isolate GD08T4 and GD866.
Genetic Analysis
Heredity
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To better understand the genetic basis of blast resistance in the wild rice species Tianjinyeshengdao(TY),F2 population from the cross between TY and the rice cultivar CO39 which is highly susceptible to blast was constructed and respectively inoculated with isolates CHL1743,110–2 and 318–2 in the growth chamber.The results showed that the resistant and susceptible plants in F2 population following inoculation fitted the ratio of 3∶1,indicating the resistance to isolates CHL1743,110–2 and 318–2 in TY was controlled by a single dominant gene which was named as Pi2–1.To preliminary determine the chromosomal location of blast resistance gene,bulked segregant analysis(BSA) and recessive-class analysis(RCA) were conducted.The results indicated that Pi2–1 was located near the centromeric region of rice chromosome 6 and linked to the markers AP5659–5 and RM7213,with a distance of 0.9 cM and 1.4 cM,respectively.
Bulked segregant analysis
Genetic Analysis
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Genetic Analysis
Genetic linkage
R gene
Cloning (programming)
Marker-Assisted Selection
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The resistance to rice blast disease in the Vietnamese traditional rice cultivar 'Chiembac' was studied. The blast resistance spectrum in 'Chiembac' and 15 rice differentials carrying different known resistance genes was identified using 25 Pyricularia grisea isolates derived from 15 AFLP lineages from the North, Center and South of Vietnam. None of the differential lines carrying a single resistance gene could effectively control all tested Vietnamese blast isolates. 'Chiembac' showed a different resistance pattern compared to that of the differential lines. A cross between 'Chiembac' and 'CR203', an improved rice cultivar, was made and the F2 population was used for characterization and mapping of the resistance genes in 'Chiembac'. Genetic analysis showed that the resistance against two representative isolates from two predominant lineages, VT7 and 12, in 'Chiembac' was controlled by the single dominant genes Pi-VT7 and Pi-I2. The resistance gene Pi-VT7 was closely linked to Pi-I2 and was mapped to chromosome 12 using the framework mapping population 'IR64' x 'Azucena' of 124 double haploid progenies. The resistance to the Vietnamese blast isolate VT7 in 'IR64' was also studied. The latter was controlled by one locus with major effect located on chromosome 12 and mapped closely to the AFLP marker NIN080, which was also tightly linked to the resistance gene Pi-VT7 in 'Chiembac'. Thus, the resistance locus Pi-VT7 and the resistance locus in 'IR64' probably belong to a cluster of resistance genes.
Doubled haploidy
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Magnaporthe
Genetic Analysis
Molecular marker
Oryza
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