Utilizing Internal Standard Responses to Assess Risk on Reporting Bioanalytical Results from Hemolyzed Samples
9
Citation
29
Reference
10
Related Paper
Citation Trend
Keywords:
Bioanalysis
Sample Preparation
Matrix (chemical analysis)
Bioanalysis
Sample Preparation
Complex matrix
Solid phase extraction
Protein precipitation
Cite
Citations (0)
Bioanalysis
Sample Preparation
Matrix (chemical analysis)
Protein precipitation
Solid phase extraction
Sample (material)
Cite
Citations (17)
The importance of sample preparation methods as the first stage in bioanalysis is described. In this article, the sample preparation concept and strategies will be discussed, along with the requirements for good sample preparation. The most widely used sample preparation methods in the pharmaceutical industry are presented; for example, the need for same-day rotation of results from large numbers of biological samples in pharmaceutical industry makes high throughput bioanalysis more essential. In this article, high-throughput sample preparation techniques are presented; examples are given of the extraction and concentration of analytes from biological matrices, including protein precipitation, solid-phase extraction, liquid–liquid extraction and microextraction-related techniques. Finally, the potential role of selective extraction methods, including molecular imprinted phases, is considered.
Sample (material)
Sample Preparation
Cite
Citations (126)
Bioanalysis
Tandem
Cite
Citations (85)
Sample preparation is a highly important and integral part of bioanalysis for cleaning up the complex biological matrices and thereby minimizing matrix effect. Matrix effect can jeopardize the precise quantification and adversely affect the reliability of liquid chromatography-mass spectrometry-based analytical results by alteration of analyte ionization. Matrix components result in suppression or enhancement of the intensity of analyte response. In spite of the high specificity and selectivity of tandem mass spectrometry, a relatively higher concentration of coeluted matrix elements present in biofluids may alter the efficiency of quantification of a bioanalytical method. Numerous literature reports different types of sample preparation techniques employed in bioanalysis. In this review, the strategies for selection of the appropriate sample clean-up technique in bioanalysis are discussed extensively. A paradigm shift in the arena of sample preparation and bioanalytical approaches involving the liquid chromatography-mass spectroscopic technique has been scrutinized. Current trends and possible future advancements in the field of biological sample extraction methods, including instrumental techniques are analyzed in detail.
Bioanalysis
Sample Preparation
Matrix (chemical analysis)
Complex matrix
Sample (material)
Cite
Citations (11)
Hydrophilic peptides present great challenges for LC-MS analysis. Although the LC-MS method provides specificity and sensitivity, sample preparation is critical for bioanalytical assay performance. A practical sample preparation approach for improving the LC-MS analysis of hydrophilic peptides was developed, using hydrophilic isoforms of the Amyloid-β peptides as an example. A typical sample preparation procedure for peptides from biological matrices employs immunoaffinity purification; however, the high content of organic solvent in the elution buffer may cause insufficient chromatographic retention of the analytes on a RPLC column. By utilizing lyophilization or speed-vacuum drying followed by reconstitution with an aqueous solvent, the subsequent LC-MS bioanalysis showed these polar peptides being retained on the RPLC column and also an improved MS response. This approach is applicable for analytical studies on hydrophilic peptides in biological matrices.
Bioanalysis
Sample Preparation
Cite
Citations (2)
This chapter reviews the most commonly used sample preparation techniques in the pharmaceutical and biopharmaceutical industry for liquid chromatography coupled with mass spectrometry (LC-MS) or tandem mass spectrometry (LC-MS/MS) bioanalyses. The chapter primarily talks about protein precipitation (PPT), liquid-liquid extraction (LLE), and solid phase extraction (SPE), focusing on the need for balancing extraction recovery and assay selectivity, and on the operational details of each technique. It explains the specificity, advantages, limitations and applications of each technique. In addition, some non-routine sample preparation techniques are discussed. It is important for bioanalytical scientists to understand the physicochemical properties (e.g. pKa, log P and log D, etc.) of the analyte(s) of interest and other needs of the intended study in order to select the most appropriate sample preparation procedures. Since 96-well plates are the most popular sample preparation format in LC-MS bioanalysis, all examples given in the chapter assume this format is used unless otherwise stated.
Bioanalysis
Sample Preparation
Protein precipitation
Solid phase extraction
Sample (material)
Biopharmaceutical
Cite
Citations (10)
Bioanalysis
Sample Preparation
Solid phase extraction
Cite
Citations (7)
Bioanalysis
Molecularly imprinted polymer
Sample Preparation
Solid phase extraction
Cite
Citations (3)
A novel approach for regulated bioanalytical sample preparation has been developed to combine multiple types of extraction techniques into one integrated and automated sample-preparation suite that pairs a graphical user interface with the Hamilton Microlab(®) STAR robotic liquid handler.The multi-assay sample-preparation suite is composed of three bioanalytical extraction techniques: protein precipitation, solid-phase extraction and liquid-liquid extraction. Validation data provided highly reproducible and robust results for each respective automated extraction technique.The user-friendly graphical user interface and modular method design provide a flexible and versatile approach for routine bioanalytical sample-preparation and is the first fully integrated multiple assay sample-preparation suite for regulated bioanalysis.
Bioanalysis
Sample Preparation
Sample (material)
Graphical user interface
Solid phase extraction
Cite
Citations (34)